{"datasetrec":{"DasID":2150,"Acronym":null,"StandardTitle":"Nannochloropsis mass cultures","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":"1","ContactEmail":null,"VersionDate":null,"VersionDay":3,"VersionMonth":4,"VersionYear":2008,"SizeReference":null,"EngAbstract":"Data describes photosynthetic behaviour of <i>Nannochloropsis</i> in a high rate algal pond and a flat panel photobioreactor","EngDescr":"<i>Nannochloropsis</i> sp. (Eustigmatophyta) is a unicellular algal species popular for mass algal cultures because it contains high concentrations of the polyunsaturated fatty acid eicosapentaenoic acid (EPA- 20:5É3). \r\nIn this experiment we followed the photosynthetic behaviour and biomass parameters of this alga during 50h in two outdoor mass cultures: a flat panel photobioreactor (FPP) and a high rate algal pond (HRAP) in order to detect acclimation patterns to different optical conditions, with the FPP having a small gradient in light intensity and the HRAP having large gradient in irradiance. \r\nAlgae of both cultures showed a depression of photosynthetic activity during the afternoon, which was more pronounced in the FPP. Despite the fact that the maximum irradiance experienced by the algae was the same in both systems, the HRAP algae showed classic shade adaptation whereas the FPP algae showed high light acclimation in photosynthetic parameters. The FPP showed diurnal changes in the maximum rate of photosynthesis and respiration and maximum rates of photosynthesis were lower at night than during the day. High temperatures (41 oC) on day 3 in the FPP caused a large reduction in Fv/Fm and an over-reduction of the PSII acceptors which did not recover during the light period. PAM measurements with and without dark adaptation showed that this decreased photosynthetic activity was due to both chronic and dynamic downregulation. Photodamage was higher in the FPP, but most damaged seem to be repaired during the evening. A comparison between the quantum efficiencies for PSII charge separation and oxygen evolution revealed a close coupling between the two.","OrigAbstract":null,"OrigDescr":null,"Comments":"A third publication about relationship between PSI and PSII activity is in prep.<Br>","ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":"English","EmbargoDate":null,"OrigDescrLangNL":"Engels","OrigLangCode":"en","OrigLangCodeExtended":"eng","OrigLangID":15,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Kromkamp J. T., Stal L. 2008: Short term variation in photosynthetic parameters of Nannochloropsis grown in two different types of outdoor mass cultures. Netherlands Institute of Ecology; Centre for Estuarine and Marine Ecology, Netherlands. Metadata available at http://mda.nioo.knaw.nl/imis.php?module=dataset&dasid=2150","AccessConstraints":"Release with permission of the appropriate parties","UDate":"2009-11-18","CDate":"2009-10-21","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-06-09 01:42:32.979540","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"StatusID":1,"DasType":"Data","DasTypeID":1,"DasOrigin":"Research","Progress":"In Progress","AccessConstraint":"Restricted","AccConstrEN":"Restricted","AccConstrDisplay":null,"License":null,"AccConstrDescription":"The data are withheld from general circulation and disclosure but access may be obtained on a case-by-case basis through negotiation","Lineage":null,"AccConID":9},"dois":null,"spcols":[{"SpColID":345,"SpName":"NIOO-CEME collection"}],"keywords":[{"ThesaurusTerm":"Mass culture","ThesTypID":1,"ThesType":"ASFA Thesaurus List","Code":null,"Description":null,"OrigThesTerm":"Mass culture","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Photosynthesis","ThesTypID":1,"ThesType":"ASFA Thesaurus List","Code":"c_5812","Description":null,"OrigThesTerm":"Photosynthesis","DutchTerm":"Fotosynthese","URI":"http://aims.fao.org/aos/agrovoc/c_5812","DasKeywordDescr":null},{"ThesaurusTerm":"Pulse amplitude modulation (PAM)","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Pulse amplitude modulation (PAM)","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Quantum efficiency","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus","Code":null,"Description":null,"OrigThesTerm":"Quantum efficiency","DutchTerm":null,"URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":null,"ownerships":[{"OrderNr":9,"Surname":null,"Firstname":null,"Initials":null,"PerPublicFlag":null,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":"nl","OrigNameLangID":41,"FullOrigName":null,"InsOwnerCNT":8,"PersID":null,"InsID":60,"FullInstitute":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; NIOZ Yerseke","RoleID":20,"Role":"Data owner","OrigName":null,"StandardName":"NIOZ Yerseke","FullAcronym":"NIOZ","ORC":null,"ROR":null}],"taxterms":[{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":37364,"TaxTerm":"Eustigmatophyceae","AphiaID":345487,"DutchTerm":null},{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":37363,"TaxTerm":"Nannochloropsis D.J.Hibberd, 1981","AphiaID":345489,"DutchTerm":null}],"frameworks":null,"otherterms":[{"OtherTerm":"Pulse amplitude modulation (PAM)"}],"temporal":[{"DasDateID":1887,"StartYear":2008,"EndYear":2008,"StartDay":1,"EndDay":3,"StartDate":"2008-04-01","EndDate":"2008-04-03","DasDate":null,"Resolution":"Hourly","ResolutionNL":"Elk uur","Notes":null,"StartMonth0":4,"StartMonth":"April","StartMonthNL":"April","EndMonth0":4,"EndMonth":"April","EndMonthNL":"April","Progress":"In Progress","ProgressNL":"Gestart"}],"geographical":[{"GeoTerm":"Eilat","DasGeoID":4479,"DasGeoTerm":null,"DasID":2150,"GeotID":7253,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":null,"Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":14842,"OrigGeoTerm":"Eilat","DutchTerm":"Eilat"}],"meastypes":[{"MethID":null,"DasMeasTypID":7140,"DasMeasType":null,"Matrix":"Biota","MatrixNL":"Biota","Parameter":"Chlorophyll a","ParameterNL":"Chlorofyl a","Description":"Chlorophyl a content","Unit":"mg/l","Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null},{"MethID":330,"DasMeasTypID":7178,"DasMeasType":null,"Matrix":"Biota","MatrixNL":"Biota","Parameter":"Fluorescent induction (OIJP transient)","ParameterNL":null,"Description":null,"Unit":null,"Methodology":"Fluorescent induction (OIJP transient) detection","Protocol":1,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"Instrument used\r\n\r\nFluorescence induction curves: A portable fluorometer, the Aquapen AP 100 (Photon System Instruments), fitted with red light-emitting diodes (LEDs) to provide saturating light intensity was used to follow the polyphasic rise of chl a fluorescence in both outdoor cultures, the so-called Kautsky curve.<br>","ParaCategoryTitle":null,"ParaCatCode":null},{"MethID":332,"DasMeasTypID":19151,"DasMeasType":null,"Matrix":"Not applicable","MatrixNL":"Niet van toepassing","Parameter":"Irradiance","ParameterNL":"Bestralingssterkte","Description":null,"Unit":"μmol quanta m-2 s-1","Methodology":"Irradiance measurement","Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":"Li-190 sensor","InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null},{"MethID":332,"DasMeasTypID":7142,"DasMeasType":null,"Matrix":"Not applicable","MatrixNL":"Niet van toepassing","Parameter":"Irradiance","ParameterNL":"Bestralingssterkte","Description":null,"Unit":"μmol quanta m-2 s-1","Methodology":"Irradiance measurement","Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":"LI1000 datalogger","InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null},{"MethID":328,"DasMeasTypID":7141,"DasMeasType":null,"Matrix":"Gas","MatrixNL":"Gas","Parameter":"Oxygen (O) evolution","ParameterNL":null,"Description":null,"Unit":"μmol O2 mg chl-1 h-1","Methodology":"Detection of changes in oxygen evolution with a Light Pipette","Protocol":1,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":"Light Pipette","InstrumentUrl":null,"MethFull":"According to Dubinsky et al (1987).\r\n\r\nThis instrument consists of a light source connected to a cuvette with a micro-oxygen electrode, a quantum sensor, a temperature-controlled water bath and a computer. It enables sensitive detection of changes in oxygen evolution/consumption and an easy collection and digitalisation of data.<br>","ParaCategoryTitle":null,"ParaCatCode":null},{"MethID":null,"DasMeasTypID":7138,"DasMeasType":null,"Matrix":"Water","MatrixNL":"Water","Parameter":"pH","ParameterNL":"pH-zuurtegraad","Description":null,"Unit":null,"Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null},{"MethID":329,"DasMeasTypID":7171,"DasMeasType":null,"Matrix":"Biota","MatrixNL":"Biota","Parameter":"Photosystem II complex (PSII) efficiency","ParameterNL":null,"Description":null,"Unit":null,"Methodology":"Photosystem II complex (PSII) efficiency measurements","Protocol":1,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"Following instruments were used:\r\n\r\nFlow-through water PAM: This is a highly sensitive chlorophyll fluorometer designed for continuously monitoring photosynthetic activity of phytoplankton and algal suspensions.\r\n\r\nDual pulse amplitude modulation (PAM): The dual PAM fluorometer (Walz) measures the Photosystem II (PSII) efficiency simultaneously with the absorption changes of Photosystem I (PSI) using the pulse modulation principle in the dual wavelength approach (Klughammer & Schreiber 2008).\r\n\r<br>","ParaCategoryTitle":null,"ParaCatCode":null},{"MethID":null,"DasMeasTypID":7172,"DasMeasType":null,"Matrix":"Biota","MatrixNL":"Biota","Parameter":"Photosystem II complex (PSII) electron transport (ETR)","ParameterNL":null,"Description":null,"Unit":"μmol e- (mg chla)-1 h-1","Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null},{"MethID":null,"DasMeasTypID":7136,"DasMeasType":null,"Matrix":"Water","MatrixNL":"Water","Parameter":"Temperature","ParameterNL":"Temperatuur","Description":null,"Unit":"°C","Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null},{"MethID":327,"DasMeasTypID":7174,"DasMeasType":null,"Matrix":"Biota","MatrixNL":"Biota","Parameter":"Total carotenoids","ParameterNL":null,"Description":null,"Unit":"mg/l","Methodology":"Methanol extraction","Protocol":1,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"According to Lichtentaler (1987)<br>","ParaCategoryTitle":null,"ParaCatCode":null}],"dasthemes":[{"DasThemeID":22,"DasTheme":"Biology > Pigments (e.g. chlorophyll)","DasTheme0":"Pigments (e.g. chlorophyll)","DutchTerm":"Pigmenten (bv. chlorophyll)"},{"DasThemeID":25,"DasTheme":"Biology > Plankton","DasTheme0":"Plankton","DutchTerm":"Plankton"},{"DasThemeID":24,"DasTheme":"Biology > Productivity - biomass","DasTheme0":"Productivity - biomass","DutchTerm":"Productiviteit - biomassa"},{"DasThemeID":36,"DasTheme":"Fisheries > Aquaculture","DasTheme0":"Aquaculture","DutchTerm":"Aquacultuur"},{"DasThemeID":14,"DasTheme":"Physical > Optical measurements","DasTheme0":"Optical measurements","DutchTerm":"Optische metingen"}],"projects":null,"refs":[{"0":141665,"BRefID":141665,"1":"Based on this dataset","Relation":"Based on this dataset","2":2,"RelationID":2,"3":"<b>Kromkamp, J.C. <i>et al.</i></b> (2009). Short-term variations in photosynthetic parameters of <i>Nannochloropsis</i> cultures grown in two types of outdoor mass cultivation systems. <i>Aquat. Microb. Ecol. 56(2-3)</i>: 309-322. <a href=\"https://dx.doi.org/10.3354/ame01318\" target=\"_blank\">https://dx.doi.org/10.3354/ame01318</a>","RR":"<b>Kromkamp, J.C. <i>et al.</i></b> (2009). Short-term variations in photosynthetic parameters of <i>Nannochloropsis</i> cultures grown in two types of outdoor mass cultivation systems. <i>Aquat. Microb. Ecol. 56(2-3)</i>: 309-322. <a href=\"https://dx.doi.org/10.3354/ame01318\" target=\"_blank\">https://dx.doi.org/10.3354/ame01318</a>","4":"Short-term variations in photosynthetic parameters of <i>Nannochloropsis</i> cultures grown in two types of outdoor mass cultivation systems","StT":"Short-term variations in photosynthetic parameters of <i>Nannochloropsis</i> cultures grown in two types of outdoor mass cultivation systems","5":"Kromkamp, J.C.; Beardall, J.; Sukenik, A.; Kopecký, J.; Masojídek, J.; van Bergeijk, S.A.; Gabai, S.; Shaham, E.; Yamshon, A.","RSA":"Kromkamp, J.C.; Beardall, J.; Sukenik, A.; Kopecký, J.; Masojídek, J.; van Bergeijk, S.A.; Gabai, S.; Shaham, E.; Yamshon, A.","6":"Gebaseerd op deze dataset","DutchTerm":"Gebaseerd op deze dataset","7":2009,"AnaDate":2009,"8":null,"MonDate":null,"9":". <i>Aquat. Microb. Ecol. 56(2-3)</i>: 309-322. <a href=\"https://dx.doi.org/10.3354/ame01318\" target=\"_blank\">https://dx.doi.org/10.3354/ame01318</a>","":". <i>Aquat. Microb. Ecol. 56(2-3)</i>: 309-322. <a href=\"https://dx.doi.org/10.3354/ame01318\" target=\"_blank\">https://dx.doi.org/10.3354/ame01318</a>","10":"https://dx.doi.org/10.3354/ame01318","doi":"https://dx.doi.org/10.3354/ame01318"},{"0":141662,"BRefID":141662,"1":"Based on this dataset","Relation":"Based on this dataset","2":2,"RelationID":2,"3":"<b>Sukenik, A. <i>et al.</i></b> (2009). Photosynthetic performance of outdoor <i>Nannochloropsis</i> mass cultures under a wide range of environmental conditions. <i>Aquat. Microb. Ecol. 56(2-3)</i>: 297-308. <a href=\"https://dx.doi.org/10.3354/ame01309\" target=\"_blank\">https://dx.doi.org/10.3354/ame01309</a>","RR":"<b>Sukenik, A. <i>et al.</i></b> (2009). Photosynthetic performance of outdoor <i>Nannochloropsis</i> mass cultures under a wide range of environmental conditions. <i>Aquat. Microb. Ecol. 56(2-3)</i>: 297-308. <a href=\"https://dx.doi.org/10.3354/ame01309\" target=\"_blank\">https://dx.doi.org/10.3354/ame01309</a>","4":"Photosynthetic performance of outdoor <i>Nannochloropsis</i> mass cultures under a wide range of environmental conditions","StT":"Photosynthetic performance of outdoor <i>Nannochloropsis</i> mass cultures under a wide range of environmental conditions","5":"Sukenik, A.; Beardall, J.; Kromkamp, J.C.; Kopecký, J.; Masojídek, J.; van Bergeijk, S.A.; Gabai, S.; Shaham, E.; Yamshon, A.","RSA":"Sukenik, A.; Beardall, J.; Kromkamp, J.C.; Kopecký, J.; Masojídek, J.; van Bergeijk, S.A.; Gabai, S.; Shaham, E.; Yamshon, A.","6":"Gebaseerd op deze dataset","DutchTerm":"Gebaseerd op deze dataset","7":2009,"AnaDate":2009,"8":null,"MonDate":null,"9":". <i>Aquat. Microb. Ecol. 56(2-3)</i>: 297-308. <a href=\"https://dx.doi.org/10.3354/ame01309\" target=\"_blank\">https://dx.doi.org/10.3354/ame01309</a>","":". <i>Aquat. Microb. Ecol. 56(2-3)</i>: 297-308. <a href=\"https://dx.doi.org/10.3354/ame01309\" target=\"_blank\">https://dx.doi.org/10.3354/ame01309</a>","10":"https://dx.doi.org/10.3354/ame01309","doi":"https://dx.doi.org/10.3354/ame01309"}],"urls":[{"URL":"mda.vliz.be/mda/directlink.php?fid=VLIZ_00000067_1256034810","externalID":"VLIZ_00000067_1256034810","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000067_1256034810","FileName":"Diurnal pH and temp.xls","Extension":"XLS"},{"URL":"mda.vliz.be/mda/directlink.php?fid=VLIZ_00000067_1256035202","externalID":"VLIZ_00000067_1256035202","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000067_1256035202","FileName":"Sukenik_ea_MEPS2009_56p297-308.pdf","Extension":"PDF"},{"URL":"mda.vliz.be/mda/directlink.php?fid=VLIZ_00000067_1256035313","externalID":"VLIZ_00000067_1256035313","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000067_1256035313","FileName":"kromkamp ea AME2009_56P309-322.pdf","Extension":"PDF"},{"URL":"mda.vliz.be/mda/directlink.php?fid=VLIZ_00000067_1256038132","externalID":"VLIZ_00000067_1256038132","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000067_1256038132","FileName":"chlacarotenoidsfinal(2).xls","Extension":"XLS"},{"URL":"mda.vliz.be/mda/directlink.php?fid=VLIZ_00000067_1256038719","externalID":"VLIZ_00000067_1256038719","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000067_1256038719","FileName":"quantum efficiencies PSII-O2 revised.xls","Extension":"XLS"},{"URL":"mda.vliz.be/mda/directlink.php?fid=VLIZ_00000067_1256038984","externalID":"VLIZ_00000067_1256038984","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000067_1256038984","FileName":"PBR_Chl-revised.xls","Extension":"XLS"},{"URL":"mda.vliz.be/mda/directlink.php?fid=VLIZ_00000067_1256039084","externalID":"VLIZ_00000067_1256039084","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000067_1256039084","FileName":"PI-parameters-revised.xls","Extension":"XLS"},{"URL":"mda.vliz.be/mda/directlink.php?fid=VLIZ_00000067_1256039168","externalID":"VLIZ_00000067_1256039168","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000067_1256039168","FileName":"SummaryBiochemicalparameters (2).xls","Extension":"XLS"},{"URL":"mda.vliz.be/mda/directlink.php?fid=VLIZ_00000067_1256039340","externalID":"VLIZ_00000067_1256039340","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000067_1256039340","FileName":"Pond_Chl_a-revised.xlsx","Extension":"XLSX"},{"URL":"mda.vliz.be/mda/directlink.php?fid=VLIZ_00000067_1256039583","externalID":"VLIZ_00000067_1256039583","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000067_1256039583","FileName":"RLC summary ex JB.xls","Extension":"XLS"}],"pictures":null,"urlmaps":null,"spatreps":null,"fileformats":null,"resmessage":"","complete":1}