{"datasetrec":{"DasID":2151,"Acronym":"microcoleus nifH","StandardTitle":"Horizontal gene transfer of a nitrogen fixation gene cluster in <i>Microcoleus chthonoplastes</i>","OrigTitle":null,"OrigTitleLangID":15,"OrigTitleLangCode":"en","OrigTitleLang":"English","OrigTitleLangNL":"Engels","VersionName":"1,0","ContactEmail":null,"VersionDate":null,"VersionDay":20,"VersionMonth":10,"VersionYear":2009,"SizeReference":null,"EngAbstract":"Genomic and experimental data showing that the nifH gene cluster in <i>Microcoleus chthonoplastes</i> is aquired via horizontal gene transfer.","EngDescr":"The filamentous, non-heterocystous cyanobacterium <i>Microcoleus chthonoplastes</i> is a cosmopolitan organism, known to build microbial mats in a variety of different environments. Although most of these cyanobacterial mats are known for their capacity to fix dinitrogen, <i>M. chthonoplastes</I> has not been assigned as a diazotrophic organism. None of the strains that were correctly identified as <i>M. chthonoplastes</i> has been shown to fix dinitrogen and it has repeatedly been reported that these organisms lacked the cyanobacterial nifH, the structural gene for dinitrogenase reductase. Here we show that a complete nif-gene cluster is present in the genome of <i>M. chthonoplastes</i> PCC 7420 and that the three structural nitrogenase genes, nifHDK, are present in a collection of axenic strains of <i>M. chthonoplastes</i> from distant locations. Phylogenetic analysis of nifHDK revealed that they cluster with the <i>Deltaproteobacteria</i> and that they are closely related to <i>Desulfovibrio</i>. The nif operon is flanked by typical cyanobacterial genes suggesting that it is an integral part of the <i>M. chthonoplastes</i> genome. Here we provide evidence that the nif operon of <i>M. chthonoplastes</i> is acquired through horizontal gene transfer. Moreover, the presence of the same nif-cluster in <i>M. chthonoplastes</i> isolates derived from various sites around the world suggests that this horizontal gene transfer event must have occurred early in the evolution of <i>M. chthonoplastes</i>. We have been unable to express nitrogenase in cultures of <i>M. chthonoplastes</i>, but we show that these genes were expressed under natural conditions in the field.","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":"English","EmbargoDate":null,"OrigDescrLangNL":"Engels","OrigLangCode":"en","OrigLangCodeExtended":"eng","OrigLangID":15,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Bolhuis H., Stal L.: Horizontal gene transfer of a nitrogen fixation gene cluster in Microcoleus chthonoplastes.Netherlands Institute of Ecology; Centre for Estuarine and Marine Ecology, Netherlands. Metadata available at http://mda.nioo.knaw.nl/imis.php?module=dataset&dasid=2151","AccessConstraints":null,"UDate":"2009-10-23","CDate":"2009-10-21","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-05-30 01:37:59.451226","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":1,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":1,"FreshFlag":0,"BrackishFlag":1,"TerrestrialFlag":0,"StatusID":1,"DasType":"Data","DasTypeID":1,"DasOrigin":"Research: experiment","Progress":"Completed","AccessConstraint":"Unrestricted for academic use","AccConstrEN":"Unrestricted for academic use","AccConstrDisplay":null,"License":null,"AccConstrDescription":"The data are freely available for research and education purposes. Usage acknowledgement is usually expected","Lineage":null,"AccConID":17},"dois":null,"spcols":[{"SpColID":345,"SpName":"NIOO-CEME collection"}],"keywords":[{"ThesaurusTerm":"Cyanobacteria","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus","Code":null,"Description":null,"OrigThesTerm":"Cyanobacteria","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Horizontal gene transfer","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Horizontal gene transfer","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Microbial mat","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Microbial mat","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Micronucleus","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Micronucleus","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Nitrogen fixation","ThesTypID":1,"ThesType":"ASFA Thesaurus List","Code":"c_5196","Description":null,"OrigThesTerm":"Nitrogen fixation","DutchTerm":null,"URI":"http://aims.fao.org/aos/agrovoc/c_5196","DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":null,"ownerships":null,"taxterms":[{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":37365,"TaxTerm":"Microcoleus chtonoplastes","AphiaID":213730,"DutchTerm":null}],"frameworks":null,"otherterms":[{"OtherTerm":"Horizontal gene transfer"},{"OtherTerm":"Microbial mat"},{"OtherTerm":"Micronucleus"}],"temporal":null,"geographical":[{"GeoTerm":"Schiermonnikoog","DasGeoID":4478,"DasGeoTerm":null,"DasID":2151,"GeotID":7252,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":null,"Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":3700,"OrigGeoTerm":"Schiermonnikoog","DutchTerm":"Schiermonnikoog"}],"meastypes":null,"dasthemes":[{"DasThemeID":23,"DasTheme":"Biology > Organic (& bio-) chemistry","DasTheme0":"Organic (& bio-) chemistry","DutchTerm":"Organisch (& bio-) chemie"}],"projects":null,"refs":null,"urls":null,"pictures":null,"urlmaps":null,"spatreps":null,"fileformats":null,"resmessage":"","complete":1}