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Taxonomic identification and cell counts are done under inverted microscope (Nikon T300E) connected to a video-interactive image analysis system ((L.U.C.I.A, Version 4.8, Laboratory Imaging Ltd, Prague) at 400x magnification by the Uteröhl (1958) method in Sedgwick-Rafter counting chambers. 400 cells are counted from each sample, while rare and large species are checked in the whole counting chamber (Moncheva and Parr, 2005/2015). The individual cell biovolume (V, μm3) is derived through the approximation of the cell shape of each species to the most similar regular solid, calculated by the respective formulas (Edier , 1979 ; Vadrucci et al., 2007),  averaged over measurement of ten cells from each species. Cell bio-volume is converted to weight (W, ng) following Hatchinson (1967). Taxonomic nomenclature is according to the on-line data-base of World Register of Marine Species (WoRMS) http://www.marinespecies.org/ and the Black Sea phytoplankton check-list. 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