{"datasetrec":{"DasID":6212,"Acronym":null,"StandardTitle":"Antarctic cryptoendolithic fungal communities ITS amplicon sequencing","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":"1.2","ContactEmail":null,"VersionDate":null,"VersionDay":19,"VersionMonth":3,"VersionYear":2019,"SizeReference":"0 records (metadata only)","EngAbstract":"Amplicon sequencing dataset of cryptoendolithic (sandstone) fungal communities (ITS1 marker gene) in Victoria Land (continental Antarctica).","EngDescr":"Rock samples were excised aseptically, transported, and stored at −20 °C at the Tuscia University (Viterbo, Italy) until processing.\r\n
\r\nSandstone rock samples were collected in triplicate in Victoria Land (Continental Antarctica), along a latitudinal transect from 74°10′10.5′′ S 162°25′38.0′′ E (Timber Peak, Northern Victoria Land) to 77°54′43.6′′ S 161°34′39.3′′ E (Finger Mt., Southern Victoria Land) ranging from 834 m a.s.l. (Battleship Promontory, Southern Victoria Land) to 3100 m a.s.l. (Mt. New Zealand, Northern Victoria Land). In addition, rocks with different sun exposures were collected from four visited sites (Battleship Promontory, Siegfried Peak, Finger Mt. and University Valley). All sites were visited during the XXXII Italian Antarctic Expedition (2015–2016).\r\n
\r\nMethod step description:\r\n
    \r\n
  1. Rocks were crushed using a Grinder MM 400 RETSCH (Verder Scientific, Bologna, Italy) in sterile conditions to avoid contamination. \r\n
  2. Metagenomic DNA was extracted from 0.3 g of rocks using MOBIO Power Soil DNA Extraction kit (MOBIO Laboratories, Carlsbad, CA, USA), according to the manufacturer’s instructions. ITS1F (CTTGGTCATTTAGAGGAAGTAA) and ITS2 (GCTGCGTTCTTCATCGATGC) primers were used to amplify the internal transcribed spacer 1 region (ITS1). PCR reactions were performed in a total volume of 25 μL, containing 1 μL of each primer, 12.5 μL of Taq DNA Polymerase (Thermo Fischer Scientific Inc., Waltham, MA, USA), 9.5 μL of nuclease-free water (Sigma-Aldrich, St. Louis, MO, USA) and 5 ng of DNA, following Coleine et al. PCR conditions were initial denaturation at 93 °C for 3 min, 35 cycles of denaturation at 95 °C for 45 s, annealing at 50 °C for 1 min, extension at 72°C for 90 s, followed by a final extension at 72 °C for 10 min in an automated thermal cycler (BioRad, Hercules, CA, USA). Amplicons, purified with Qiagen PCR CleanUp kit (Macherey-Nagel, Hoerdt, France) and quantified using the Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA, USA), were tagged with unique barcodes to enable identification of each sample, and then pooled for run sequencing. \r\n
  3. Sequencing (paired-end reads, 2 × 300 bp) of the pooled libraries was performed on a single Illumina MiSeq flowcell at the Institute for Integrative Genome Biology, University of California, Riverside.\r\n
","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Coleine C, Zucconi L, Onofri S, Pombubpa N, Stajich J, Selbman L (2018): Antarctic cryptoendolithic fungal communities ITS amplicon sequencing. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. Https://ipt.biodiversity.aq/resource?r=antarctic_cryptoendolithic_microbial_fungi_its&v=1.2","AccessConstraints":null,"UDate":"2019-04-10","CDate":"2019-03-28","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-05-14 01:44:00.153333","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":1,"StatusID":1,"DasType":"Metadata","DasTypeID":8,"DasOrigin":"Research: field survey","Progress":"Completed","AccessConstraint":"Attribution (CC BY)","AccConstrEN":"Attribution (CC BY)","AccConstrDisplay":"\"Creative This dataset is licensed under a Creative Commons Attribution 4.0 International License.","License":"https://creativecommons.org/licenses/by/4.0/","AccConstrDescription":"This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.","Lineage":null,"AccConID":21,"DOI":"10.15468/drprsj","GBIF_UUID":"b94f714c-e890-4365-81a5-968a20606d37"},"dois":null,"spcols":null,"keywords":[{"ThesaurusTerm":"Antarctica, Victoria Land","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"Antarctica, Victoria Land","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Dna sequencing","ThesTypID":4,"ThesType":"CAB Thesaurus","Code":"40176","Description":null,"OrigThesTerm":"Dna sequencing","DutchTerm":null,"URI":"https://id.cabi.org/cabt/40176","DasKeywordDescr":null},{"ThesaurusTerm":"Metadata","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus","Code":null,"Description":null,"OrigThesTerm":"Metadata","DutchTerm":null,"URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":[{"DasID":1543,"Acronym":"AntOBIS","StandardTitle":"Antarctic Ocean Biodiversity Information System","Relation":"Published dataset","RelationY":"Published in","RelID":13,"doi":null,"vlizDoi":null},{"DasID":5816,"Acronym":"RAS","StandardTitle":"Register of Antarctic Species","Relation":"Partial Source DataSet","RelationY":"Partly included in","RelID":8,"doi":null,"vlizDoi":null}],"ownerships":[{"OrderNr":1,"Surname":"Coleine","Firstname":"Claudia","Initials":"C.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":"it","OrigNameLangID":32,"FullOrigName":"Università della Tuscia - Viterbo","InsOwnerCNT":5,"PersID":37290,"InsID":8773,"FullInstitute":"University of Tuscia - Viterbo","RoleID":61,"Role":"Data creator","OrigName":"Università della Tuscia - Viterbo","StandardName":"University of Tuscia - Viterbo","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":2,"Surname":"Zucconi","Firstname":"Laura","Initials":"L.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":"it","OrigNameLangID":32,"FullOrigName":"Università della Tuscia - Viterbo","InsOwnerCNT":5,"PersID":37291,"InsID":8773,"FullInstitute":"University of Tuscia - Viterbo","RoleID":61,"Role":"Data creator","OrigName":"Università della Tuscia - Viterbo","StandardName":"University of Tuscia - Viterbo","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":3,"Surname":"Onofri","Firstname":"Silvano","Initials":"S.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":"it","OrigNameLangID":32,"FullOrigName":"Università della Tuscia - Viterbo","InsOwnerCNT":5,"PersID":37292,"InsID":8773,"FullInstitute":"University of Tuscia - Viterbo","RoleID":61,"Role":"Data creator","OrigName":"Università della Tuscia - Viterbo","StandardName":"University of Tuscia - Viterbo","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":4,"Surname":"Pombubpa","Firstname":"Nuttapon","Initials":"N.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":2,"PersID":37293,"InsID":14284,"FullInstitute":"University of California, Riverside","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"University of California, Riverside","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":5,"Surname":"Stajich","Firstname":"Jason","Initials":"J.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":2,"PersID":37294,"InsID":14284,"FullInstitute":"University of California, Riverside","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"University of California, Riverside","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":6,"Surname":"Selbmann","Firstname":"Laura","Initials":"L.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":"it","OrigNameLangID":32,"FullOrigName":"Università della Tuscia - Viterbo","InsOwnerCNT":5,"PersID":37295,"InsID":8773,"FullInstitute":"University of Tuscia - Viterbo","RoleID":61,"Role":"Data creator","OrigName":"Università della Tuscia - Viterbo","StandardName":"University of Tuscia - Viterbo","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":7,"Surname":"Coleine","Firstname":"Claudia","Initials":"C.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":"it","OrigNameLangID":32,"FullOrigName":"Università della Tuscia - Viterbo","InsOwnerCNT":5,"PersID":37290,"InsID":8773,"FullInstitute":"University of Tuscia - Viterbo","RoleID":24,"Role":"Contact","OrigName":"Università della Tuscia - Viterbo","StandardName":"University of Tuscia - Viterbo","FullAcronym":null,"ORC":null,"ROR":null}],"taxterms":[{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":11312,"TaxTerm":"Fungi","AphiaID":4,"DutchTerm":"Schimmels"}],"frameworks":null,"otherterms":null,"temporal":[{"DasDateID":4959,"StartYear":2015,"EndYear":2016,"StartDay":null,"EndDay":null,"StartDate":"2015-01-01","EndDate":"2016-01-01","DasDate":null,"Resolution":null,"ResolutionNL":null,"Notes":null,"StartMonth0":null,"StartMonth":null,"StartMonthNL":null,"EndMonth0":null,"EndMonth":null,"EndMonthNL":null,"Progress":"Completed","ProgressNL":"Afgelopen"}],"geographical":[{"GeoTerm":"Victoria Land","DasGeoID":10339,"DasGeoTerm":null,"DasID":6212,"GeotID":547,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":null,"Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":13871,"OrigGeoTerm":"Victoria Land","DutchTerm":null}],"meastypes":[{"MethID":null,"DasMeasTypID":12791,"DasMeasType":"","Matrix":null,"MatrixNL":null,"Parameter":"Molecular data","ParameterNL":"Moleculaire data","Description":null,"Unit":null,"Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null}],"dasthemes":null,"projects":null,"refs":null,"urls":[{"URL":"https://dx.doi.org/10.15468/drprsj","externalID":"10.15468/drprsj","URLTypeCode":"DOI","URLType":"DOI","URLTypID":13,"downloadURL":null,"FileName":null},{"URL":"https://www.gbif.org/dataset/b94f714c-e890-4365-81a5-968a20606d37","externalID":"b94f714c-e890-4365-81a5-968a20606d37","URLTypeCode":"GBIF_UUID","URLType":"GBIF dataset","URLTypID":43,"downloadURL":null,"FileName":null},{"URL":"https://ipt.biodiversity.aq/resource?r=antarctic_cryptoendolithic_microbial_fungi_its","externalID":null,"URLTypeCode":null,"URLType":"IPT","URLTypID":33,"downloadURL":null,"FileName":null},{"URL":"https://obis.org/dataset/ac23ec5d-19a6-46a5-8b7b-e851125ebc76","externalID":"ac23ec5d-19a6-46a5-8b7b-e851125ebc76","URLTypeCode":null,"URLType":"Online dataset","URLTypID":19,"downloadURL":null,"FileName":null}],"pictures":null,"urlmaps":null,"spatreps":null,"fileformats":null,"resmessage":"","complete":1}