{"datasetrec":{"DasID":6230,"Acronym":null,"StandardTitle":"Global biogeography of desert cyanobacteria","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":"1.4","ContactEmail":null,"VersionDate":null,"VersionDay":19,"VersionMonth":3,"VersionYear":2019,"SizeReference":"0 records (metadata only)","EngAbstract":"Amplicon sequencing dataset (454 pyrosequencing) of Bacteria (16S ssu rRNA) and Cyanobacteria (nifH) in cold desert quartz rocks.","EngDescr":"Colonized quartz stones were retrieved by hand (using isopropyl alcohol surface sterilized latex gloves) and loose soil particles gently removed with a sterile (autoclaved) paintbrush. Samples were then stored in sterile Whirlpak bags (Nasco) at -20°C in the field and in transit, and subsequently stored frozen at -80°C in the laboratory until processed.\r\n<br>\r\nStudy Extent: Hypolithic communities were recovered from quartz substrate in desert pavement on the Tibetan Plateau (China), the Taklimakan Desert (China), Devon Island (Canada) and the McMurdo Dry Valleys (Antarctica).\r\n<br>\r\nMethod step description:\r\n<ol>\r\n<li>Amplification of 16S rRNA genes was achieved using primer pair 341F and 907R with PCR conditions involving an initial denaturation time of 5min; 30 cycles at 95°C for 1 min, 55°C for 1 min, 72°C for 1 min, and a final extension at 72°C for 10 min. Positive and negative controls were run for every PCR. For each amplicon library purification was carried out with Agencourt AMPure XP Bead (Beckman Coulter, CA, USA) according to manufacturer’s instructions. The libraries were quantified with Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen Life Technologies, NY, USA) using FLUOstar OPTIMA F fluorometer (BMG Labtech GmbH, Offenburg, Germany) and library quality was assessed with the FlashGel System (Lonza Group Ltd., Basel, Switzerland). \r\n<li>Emulsion-PCR was carried out with GS Junior Titanium emPCR Kit (Lib-L, 454 Life Sciences Corp., CT, USA) according to the emPCR Amplification Method Manual – Lib-L, Single-Prep. The sequencing reaction was carried out with the GS Junior Titanium Sequencing Kit and GS Junior Titanium PicoTiterPlate Kit (454 Life Sciences Corp.) according to the manufacturer’s instructions. The sequencing run was conducted in 200 cycles.\r\n</ol>","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Lacap-bugler D, Lee K, Archer S, Gillman L, Lau M, Leuzinger S, Lee C, Maki T, McKay C, Perrott J, de los Rios-Murillo A, Warren-Rhodes K, Hopkins D, Pointing S (2018): Global biogeography of desert cyanobacteria. v1.4. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=desert_cyanobacteria&v=1.4","AccessConstraints":null,"UDate":"2019-04-10","CDate":"2019-04-03","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-05-23 01:39:24.004480","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":1,"StatusID":1,"DasType":"Metadata","DasTypeID":8,"DasOrigin":"Research: field survey","Progress":"Completed","AccessConstraint":"Attribution (CC BY)","AccConstrEN":"Attribution (CC BY)","AccConstrDisplay":"<a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\"><img alt=\"Creative Commons License\" style=\"border:0px;height:15px;width:80px;vertical-align:middle;\" src=\"https://www.marinespecies.org/aphia/images/cc/by.png\" /></a> This dataset is licensed under a <a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution 4.0 International License</a>.","License":"https://creativecommons.org/licenses/by/4.0/","AccConstrDescription":"This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.","Lineage":null,"AccConID":21,"DOI":"10.15468/r3ndq1","GBIF_UUID":"914f39c7-7bb2-4e77-a4ae-36147aa07daf"},"dois":null,"spcols":null,"keywords":[{"ThesaurusTerm":"Antarctica, Victoria Land, McMurdo Dry Valleys","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"Antarctica, Victoria Land, McMurdo Dry Valleys","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Dna sequencing","ThesTypID":4,"ThesType":"CAB Thesaurus","Code":"40176","Description":null,"OrigThesTerm":"Dna sequencing","DutchTerm":null,"URI":"https://id.cabi.org/cabt/40176","DasKeywordDescr":null},{"ThesaurusTerm":"Metadata","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus","Code":null,"Description":null,"OrigThesTerm":"Metadata","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"PN, Arctic","ThesTypID":34,"ThesType":"ASFA 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