{"datasetrec":{"DasID":6236,"Acronym":null,"StandardTitle":"Microbes (16S and 18S rRNA genes) from benthic Antarctic sediments","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":"1.2","ContactEmail":null,"VersionDate":null,"VersionDay":19,"VersionMonth":3,"VersionYear":2019,"SizeReference":"0 records (metadata only)","EngAbstract":"Amplicon sequencing dataset (Illumina MiSeq) of microbial communities (16S and 18S rRNA genes) in 24 samples from benthic Antarctic sediments.","EngDescr":"Samples were collected on the Antarctic shelf at depths ranging from 223 to 820 m. The top 3 cm of sediments were transferred into sampling tubes and stored frozen (−80°C). Samples were shipped frozen to Central Michigan University within 3 months of collection.\r\n<br>\r\nStudy Extent: Surface sediment samples were collected from the continental shelf of Antarctica during two research cruises. The first cruise (Dec. 2013–Feb. 2014, RVIB Nathaniel B. Palmer) sampled Western Antarctica, which includes the Amundsen Sea, Bellingshausen Sea, and Ross Sea, using a multicorer. The second cruise (Nov.–Dec. 2014, ASRV Laurence M. Gould) sampled the Antarctic Peninsula using a box corer.\r\n<br>\r\nMethod step description:\r\n<ol>\r\n<li>DNA was extracted using a PowerSoil DNA extraction kit (MoBio) following the manufacturer's protocol. Approximately 4–8 extractions were completed on each sediment sample and pooled and concentrated with a DNA Clean and Concentrator kit (Zymo) due to low yields from some of the samples. DNA was then quantified using the Qubit2.0 Fluorometer (Life Technologies) and stored at −20°C. Bacterial and archaeal sequences were generated from the V4 region of 16S rRNA gene using the primer set 515f and 806r while eukaryotic sequences were obtained with the V4 region of the 18S rRNA gene using the primer set 1391r and EukBR. Resulting amplicons were sequenced on an Illumina MiSeq as paired-end (PE) reads of 250-bp at Michigan State University's (MSU) Research Technology Support Facility (RTSF) Genomics Core.\r\n</ol>","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Learman D, Henson M, Thrash C, Temperton B, Brannock P, Santos S, Mahon A, Halanych K (2019): Microbes (16S and 18S rRNA genes) from benthic Antarctic sediments. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_in_benthic_antarctic_sediments&v=1.2","AccessConstraints":null,"UDate":"2019-04-10","CDate":"2019-04-03","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-05-14 01:44:00.153333","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"StatusID":1,"DasType":"Metadata","DasTypeID":8,"DasOrigin":"Research: field survey","Progress":"Completed","AccessConstraint":"Attribution (CC BY)","AccConstrEN":"Attribution (CC BY)","AccConstrDisplay":"<a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\"><img alt=\"Creative Commons License\" style=\"border:0px;height:15px;width:80px;vertical-align:middle;\" src=\"https://www.marinespecies.org/aphia/images/cc/by.png\" /></a> This dataset is licensed under a <a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution 4.0 International License</a>.","License":"https://creativecommons.org/licenses/by/4.0/","AccConstrDescription":"This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.","Lineage":null,"AccConID":21,"DOI":"10.15468/6heyxn","GBIF_UUID":"3a0fa23f-411a-49ec-96ed-3ee8cf7d5696"},"dois":null,"spcols":null,"keywords":[{"ThesaurusTerm":"Dna sequencing","ThesTypID":4,"ThesType":"CAB Thesaurus","Code":"40176","Description":null,"OrigThesTerm":"Dna sequencing","DutchTerm":null,"URI":"https://id.cabi.org/cabt/40176","DasKeywordDescr":null},{"ThesaurusTerm":"Metadata","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus","Code":null,"Description":null,"OrigThesTerm":"Metadata","DutchTerm":null,"URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":[{"DasID":1543,"Acronym":"AntOBIS","StandardTitle":"Antarctic Ocean Biodiversity Information System","Relation":"Published dataset","RelationY":"Published in","RelID":13,"doi":null,"vlizDoi":null},{"DasID":5816,"Acronym":"RAS","StandardTitle":"Register of Antarctic Species","Relation":"Partial Source DataSet","RelationY":"Partly included in","RelID":8,"doi":null,"vlizDoi":null}],"ownerships":[{"OrderNr":1,"Surname":"Learman","Firstname":"Deric","Initials":"D.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"CMU","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":3,"PersID":37413,"InsID":14297,"FullInstitute":"Central Michigan University","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Central Michigan University","FullAcronym":"CMU","ORC":null,"ROR":null},{"OrderNr":2,"Surname":"Henson","Firstname":"Michael","Initials":"M.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"LSU","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":2,"PersID":37414,"InsID":10377,"FullInstitute":"Louisiana State University","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Louisiana State University","FullAcronym":"LSU","ORC":null,"ROR":null},{"OrderNr":3,"Surname":"Thrash","Firstname":"Cameron","Initials":"C.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"LSU","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":2,"PersID":37415,"InsID":10377,"FullInstitute":"Louisiana State University","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Louisiana State University","FullAcronym":"LSU","ORC":null,"ROR":null},{"OrderNr":4,"Surname":"Temperton","Firstname":"Ben","Initials":"B.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":1,"PersID":37416,"InsID":8040,"FullInstitute":"University of Exeter","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"University of Exeter","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":5,"Surname":"Brannock","Firstname":"Pamela","Initials":"P.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":3,"PersID":37417,"InsID":5721,"FullInstitute":"Auburn University","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Auburn University","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":6,"Surname":"Santos","Firstname":"Scott","Initials":"S.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":3,"PersID":37418,"InsID":5721,"FullInstitute":"Auburn University","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Auburn University","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":7,"Surname":"Mahon","Firstname":"Andrew","Initials":"A.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"CMU","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":3,"PersID":37419,"InsID":14297,"FullInstitute":"Central Michigan University","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Central Michigan University","FullAcronym":"CMU","ORC":null,"ROR":null},{"OrderNr":8,"Surname":"Halanych","Firstname":"Kenneth","Initials":"K.M.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":3,"PersID":19972,"InsID":5721,"FullInstitute":"Auburn University","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Auburn University","FullAcronym":null,"ORC":"https://orcid.org/0000-0002-8658-9674","ROR":null},{"OrderNr":9,"Surname":"Learman","Firstname":"Deric","Initials":"D.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"CMU","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":3,"PersID":37413,"InsID":14297,"FullInstitute":"Central Michigan University","RoleID":24,"Role":"Contact","OrigName":null,"StandardName":"Central Michigan University","FullAcronym":"CMU","ORC":null,"ROR":null}],"taxterms":[{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":39154,"TaxTerm":"archaea","AphiaID":8,"DutchTerm":null},{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":11315,"TaxTerm":"Bacteria","AphiaID":6,"DutchTerm":null}],"frameworks":null,"otherterms":null,"temporal":[{"DasDateID":4983,"StartYear":2014,"EndYear":2014,"StartDay":null,"EndDay":null,"StartDate":"2014-01-01","EndDate":"2014-01-01","DasDate":null,"Resolution":null,"ResolutionNL":null,"Notes":null,"StartMonth0":null,"StartMonth":null,"StartMonthNL":null,"EndMonth0":null,"EndMonth":null,"EndMonthNL":null,"Progress":"Completed","ProgressNL":"Afgelopen"}],"geographical":[{"GeoTerm":"Southern Ocean","DasGeoID":10372,"DasGeoTerm":null,"DasID":6236,"GeotID":6491,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":null,"Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":1907,"OrigGeoTerm":"Southern Ocean","DutchTerm":"Zuidelijke Oceaan"}],"meastypes":[{"MethID":null,"DasMeasTypID":12814,"DasMeasType":"","Matrix":null,"MatrixNL":null,"Parameter":"Molecular data","ParameterNL":"Moleculaire data","Description":null,"Unit":null,"Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null}],"dasthemes":null,"projects":null,"refs":null,"urls":[{"URL":"https://doi.org/10.15468/6heyxn","externalID":"10.15468/6heyxn","URLTypeCode":"DOI","URLType":"DOI","URLTypID":13,"downloadURL":null,"FileName":null},{"URL":"https://www.gbif.org/dataset/3a0fa23f-411a-49ec-96ed-3ee8cf7d5696","externalID":"3a0fa23f-411a-49ec-96ed-3ee8cf7d5696","URLTypeCode":"GBIF_UUID","URLType":"GBIF dataset","URLTypID":43,"downloadURL":null,"FileName":null},{"URL":"https://ipt.biodiversity.aq/resource?r=microbes_in_benthic_antarctic_sediments","externalID":null,"URLTypeCode":null,"URLType":"IPT","URLTypID":33,"downloadURL":null,"FileName":null},{"URL":"https://obis.org/dataset/47812de1-4177-49b0-ae20-3384c62fb55d","externalID":"47812de1-4177-49b0-ae20-3384c62fb55d","URLTypeCode":null,"URLType":"Online dataset","URLTypID":19,"downloadURL":null,"FileName":null}],"pictures":null,"urlmaps":null,"spatreps":null,"fileformats":null,"resmessage":"","complete":1}