{"datasetrec":{"DasID":6241,"Acronym":null,"StandardTitle":"Microbial communities (Bacteria, Eukaryotes and Fungi) in Arctic, Antarctic and Sub-Antarctic lacustrine biofilms","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":"1.1","ContactEmail":null,"VersionDate":null,"VersionDay":19,"VersionMonth":3,"VersionYear":2019,"SizeReference":"0 records (metadata only)","EngAbstract":"Amplicon sequencing (Illumina MiSeq, 300bp Paired-end) dataset of microbial mats samples collected in the literal zone of Arctic, Antarctic and Sub-Antarctic lakes. Samples were taken during the course of field expeditions between 1993 and 2014. Sequencing targeted Bacteria (16S ssu rRNA marker gene, v1-v3 region), eukaryotes (18S ssu rRNA, v4 region) and microbial fungi (ITS marker gene).","EngDescr":"Microbial mat samples were collected in the littoral or deeper parts of the euphotic zone of the lakes using a spatula or gravity corer, respectively. The upper 1 cm of the core was aseptically removed and kept dark and cool until transfer to -20°C.\r\n<br>\r\nStudy Extent: Samples were taken from benthic microbial mats (upper 1 cm), collected in 233 lakes covering 17.35° latitude in the Northern Hemisphere (61.39°N to 78.74°N) and 37.16° in the Southern Hemisphere (46.84°S to 84°S), including the major biogeographical regions in Antarctica, Sub-Antarctica and the Arctic.\r\n<br>\r\nQuality Control\r\n<ol>\r\n<li>To assess overall sequence quality and estimate the parameters for bioinformatics processing, each run also included two replicates of positive control sample (mock community) containing bacterial or eukaryote taxa on the respective bacterial and eukaryote runs. \r\nMethod step description:\r\nExtracellular proteins and DNA from 1.5-3 g subsamples were removed (Corinaldesi et al., 2005), after which genomic DNA was extracted using a phenol-chloroform based protocol (Zwart et al., 1998). For Bacteria, the V1-V3 region of the 16S SSU rRNA gene was targeted for bacteria with domain-specific universal primer sets (Edwards et al., 1989; Cleenwerck et al., 2007), while for eukaryotes, V4 region of the 18S ssu rRNA gene was targeted with the primers of Stoeck et al. (2010). . The polymerase chain reaction was performed in duplicate to level out stochastic artefacts, and amplicon libraries were barcoded using the NEXTERA XT index kit (Illumina Inc.) according to manufacturer's instructions. Libraries were sequenced on an Illumina MiSeq machine (300 bp, Paired-end), while each run was spiked with 20% PhiX DNA (Illumina Inc.) to reduce cluster density.\r\n</ol>","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Sweetlove M, Tytgat B, Verleyen E, Willems A, Wurzbacher C, Nillson H, Wilmotte A, Vyverman W (2019): Microbial communities (Bacteria, Eukaryotes and Fungi) in Arctic, Antarctic and Sub-Antarctic lacustrine biofilms. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbial_bacteria_fungi_and_eukaryotes_in_arctic_antarctic_and_subantarctic_lacustrine_biofilms&v=1.1","AccessConstraints":null,"UDate":"2019-04-10","CDate":"2019-04-03","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-05-14 01:44:00.153333","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":0,"FreshFlag":1,"BrackishFlag":0,"TerrestrialFlag":0,"StatusID":1,"DasType":"Metadata","DasTypeID":8,"DasOrigin":"Research: field survey","Progress":"Completed","AccessConstraint":"Attribution (CC BY)","AccConstrEN":"Attribution (CC BY)","AccConstrDisplay":"<a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\"><img alt=\"Creative Commons License\" style=\"border:0px;height:15px;width:80px;vertical-align:middle;\" src=\"https://www.marinespecies.org/aphia/images/cc/by.png\" /></a> This dataset is licensed under a <a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution 4.0 International License</a>.","License":"https://creativecommons.org/licenses/by/4.0/","AccConstrDescription":"This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.","Lineage":null,"AccConID":21,"DOI":"10.15468/c5mt5n","GBIF_UUID":"81484676-d49f-43f5-8f78-88289267664b"},"dois":null,"spcols":null,"keywords":[{"ThesaurusTerm":"Antarctica","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"Antarctica","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Antarctica, Antarctic Peninsula","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"Antarctica, Antarctic Peninsula","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Dna sequencing","ThesTypID":4,"ThesType":"CAB Thesaurus","Code":"40176","Description":null,"OrigThesTerm":"Dna sequencing","DutchTerm":null,"URI":"https://id.cabi.org/cabt/40176","DasKeywordDescr":null},{"ThesaurusTerm":"Lake shores","ThesTypID":1,"ThesType":"ASFA Thesaurus List","Code":null,"Description":null,"OrigThesTerm":"Lake shores","DutchTerm":"Meeroevers","URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Macquarie I.","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"Macquarie I.","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Metadata","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus","Code":null,"Description":null,"OrigThesTerm":"Metadata","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Microbial mats","ThesTypID":1,"ThesType":"ASFA Thesaurus List","Code":null,"Description":null,"OrigThesTerm":"Microbial mats","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"PN, Arctic","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"PN, Arctic","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"PSW, South Africa, Prince Edward I., Marion I.","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"PSW, South Africa, Prince Edward I., Marion I.","DutchTerm":null,"URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":[{"DasID":1543,"Acronym":"AntOBIS","StandardTitle":"Antarctic Ocean Biodiversity Information System","Relation":"Published dataset","RelationY":"Published in","RelID":13,"doi":null,"vlizDoi":null},{"DasID":5816,"Acronym":"RAS","StandardTitle":"Register of Antarctic Species","Relation":"Partial Source DataSet","RelationY":"Partly included in","RelID":8,"doi":null,"vlizDoi":null}],"ownerships":[{"OrderNr":2,"Surname":"Tytgat","Firstname":"Bjorn","Initials":"B.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"UGent","OrigNameLangCode":"en","OrigNameLangID":15,"FullOrigName":"Ghent University","InsOwnerCNT":7,"PersID":33305,"InsID":471,"FullInstitute":"Universiteit Gent","RoleID":61,"Role":"Data creator","OrigName":"Ghent University","StandardName":"Universiteit Gent","FullAcronym":"UGent","ORC":"https://orcid.org/0000-0002-6926-3488","ROR":null},{"OrderNr":3,"Surname":"Verleyen","Firstname":"Elie","Initials":"E.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"UGent","OrigNameLangCode":"en","OrigNameLangID":15,"FullOrigName":"Ghent University","InsOwnerCNT":7,"PersID":3395,"InsID":471,"FullInstitute":"Universiteit Gent","RoleID":61,"Role":"Data creator","OrigName":"Ghent University","StandardName":"Universiteit Gent","FullAcronym":"UGent","ORC":"https://orcid.org/0000-0003-1426-2960","ROR":null},{"OrderNr":4,"Surname":"Willems","Firstname":"Anne","Initials":"A.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"UGent","OrigNameLangCode":"en","OrigNameLangID":15,"FullOrigName":"Ghent University","InsOwnerCNT":7,"PersID":10817,"InsID":471,"FullInstitute":"Universiteit Gent","RoleID":61,"Role":"Data creator","OrigName":"Ghent University","StandardName":"Universiteit Gent","FullAcronym":"UGent","ORC":"https://orcid.org/0000-0002-8421-2881","ROR":null},{"OrderNr":5,"Surname":"Wurzbacher","Firstname":"Christian","Initials":"C.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"TUM","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":1,"PersID":37443,"InsID":12034,"FullInstitute":"Technische Universität München","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Technische Universität München","FullAcronym":"TUM","ORC":null,"ROR":null},{"OrderNr":6,"Surname":"Nillson","Firstname":"Henrik","Initials":"H.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":"sv","OrigNameLangID":53,"FullOrigName":"Göteborgs universitet","InsOwnerCNT":1,"PersID":37444,"InsID":7779,"FullInstitute":"University of Gothenburg","RoleID":61,"Role":"Data creator","OrigName":"Göteborgs universitet","StandardName":"University of Gothenburg","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":7,"Surname":"Wilmotte","Firstname":"Annick","Initials":"A.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"ULG","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":1,"PersID":4519,"InsID":467,"FullInstitute":"Université de Liège","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Université de Liège","FullAcronym":"ULG","ORC":"https://orcid.org/0000-0003-3546-3489","ROR":null},{"OrderNr":8,"Surname":"Vyverman","Firstname":"Wim","Initials":"W.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"UGent","OrigNameLangCode":"en","OrigNameLangID":15,"FullOrigName":"Ghent University","InsOwnerCNT":7,"PersID":135,"InsID":471,"FullInstitute":"Universiteit Gent","RoleID":61,"Role":"Data creator","OrigName":"Ghent University","StandardName":"Universiteit Gent","FullAcronym":"UGent","ORC":"https://orcid.org/0000-0003-0850-2569","ROR":null},{"OrderNr":9,"Surname":"Vyverman","Firstname":"Wim","Initials":"W.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"UGent","OrigNameLangCode":"en","OrigNameLangID":15,"FullOrigName":"Ghent University","InsOwnerCNT":7,"PersID":135,"InsID":471,"FullInstitute":"Universiteit Gent","RoleID":24,"Role":"Contact","OrigName":"Ghent University","StandardName":"Universiteit Gent","FullAcronym":"UGent","ORC":"https://orcid.org/0000-0003-0850-2569","ROR":null},{"OrderNr":10,"Surname":"Verleyen","Firstname":"Elie","Initials":"E.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"UGent","OrigNameLangCode":"en","OrigNameLangID":15,"FullOrigName":"Ghent University","InsOwnerCNT":7,"PersID":3395,"InsID":471,"FullInstitute":"Universiteit Gent","RoleID":24,"Role":"Contact","OrigName":"Ghent University","StandardName":"Universiteit Gent","FullAcronym":"UGent","ORC":"https://orcid.org/0000-0003-1426-2960","ROR":null}],"taxterms":[{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":11315,"TaxTerm":"Bacteria","AphiaID":6,"DutchTerm":null},{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":11312,"TaxTerm":"Fungi","AphiaID":4,"DutchTerm":"Schimmels"}],"frameworks":null,"otherterms":null,"temporal":[{"DasDateID":4988,"StartYear":1993,"EndYear":2014,"StartDay":null,"EndDay":null,"StartDate":"1993-01-01","EndDate":"2014-01-01","DasDate":null,"Resolution":null,"ResolutionNL":null,"Notes":null,"StartMonth0":null,"StartMonth":null,"StartMonthNL":null,"EndMonth0":null,"EndMonth":null,"EndMonthNL":null,"Progress":"Completed","ProgressNL":"Afgelopen"}],"geographical":[{"GeoTerm":"Antarctic Peninsula","DasGeoID":10381,"DasGeoTerm":null,"DasID":6241,"GeotID":497,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":null,"Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":9978,"OrigGeoTerm":"Antarctic Peninsula","DutchTerm":null},{"GeoTerm":"Antarctica","DasGeoID":10382,"DasGeoTerm":null,"DasID":6241,"GeotID":496,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":null,"Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":1926,"OrigGeoTerm":"Antarctica","DutchTerm":"Antarctica"},{"GeoTerm":"Arctic Ocean","DasGeoID":10378,"DasGeoTerm":null,"DasID":6241,"GeotID":4602,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":"Greenland, Norway, Svalbard","Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":1906,"OrigGeoTerm":"Arctic Ocean","DutchTerm":"Arctisch"},{"GeoTerm":"Macquarie Island","DasGeoID":10379,"DasGeoTerm":null,"DasID":6241,"GeotID":4867,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":null,"Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":8592,"OrigGeoTerm":"Macquarie Island","DutchTerm":null},{"GeoTerm":"Marion Island","DasGeoID":10380,"DasGeoTerm":null,"DasID":6241,"GeotID":5121,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":null,"Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":22130,"OrigGeoTerm":"Marion Island","DutchTerm":null}],"meastypes":[{"MethID":null,"DasMeasTypID":12819,"DasMeasType":"","Matrix":null,"MatrixNL":null,"Parameter":"Molecular data","ParameterNL":"Moleculaire data","Description":null,"Unit":null,"Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null}],"dasthemes":null,"projects":null,"refs":null,"urls":[{"URL":"https://doi.org/10.15468/c5mt5n","externalID":"10.15468/c5mt5n","URLTypeCode":"DOI","URLType":"DOI","URLTypID":13,"downloadURL":null,"FileName":null},{"URL":"https://www.gbif.org/dataset/81484676-d49f-43f5-8f78-88289267664b","externalID":"81484676-d49f-43f5-8f78-88289267664b","URLTypeCode":"GBIF_UUID","URLType":"GBIF dataset","URLTypID":43,"downloadURL":null,"FileName":null},{"URL":"https://ipt.biodiversity.aq/resource?r=microbial_bacteria_fungi_and_eukaryotes_in_arctic_antarctic_and_subantarctic_lacustrine_biofilms","externalID":null,"URLTypeCode":null,"URLType":"IPT","URLTypID":33,"downloadURL":null,"FileName":null},{"URL":"https://obis.org/dataset/3c2b9217-ab83-4f3a-8ff3-e752e1ee459e","externalID":"3c2b9217-ab83-4f3a-8ff3-e752e1ee459e","URLTypeCode":null,"URLType":"Online dataset","URLTypID":19,"downloadURL":null,"FileName":null}],"pictures":null,"urlmaps":null,"spatreps":null,"fileformats":null,"resmessage":"","complete":1}