{"datasetrec":{"DasID":6267,"Acronym":null,"StandardTitle":"V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":"1.1","ContactEmail":null,"VersionDate":null,"VersionDay":19,"VersionMonth":3,"VersionYear":2019,"SizeReference":"0 records (metadata only)","EngAbstract":"Amplicon sequencing dataset (Illumina MiSeq 250bp PE) of soil bacteria (16S ssu rRNA gene, v3-v4) on King George Island and Deception Island (Antarctica).","EngDescr":"Samples were stored at –20°C immediately after sampling.\r\n<br>\r\nStudy Extent: Soil samples were collected from King George Island (S62°11′35.3′′; W58°56′6.2′′) and Deception Island (S62°59′023′′; W60°40′51.7′′), Antarctica in February 2007.\r\n<br>\r\nMethod step description:\r\n<ol>\r\n<li>Total bacterial genomic DNA was extracted from soils using the method described in Zhou et al. (1996) DNA recovery from soils of diverse composition. Appl. Environ. Microbiol., 62(2), 316–322. \r\n<li>The concentration and purity of the extracted genomic DNA were measured using the NanoDropTM 2000 spectrophotometer (Thermo Scientific Inc., Waltham, MA, USA). \r\n<li>The V3–V4 region of the bacterial 16S rRNA gene was amplified using locus-specific primers, S-DBact-0341-b- S-17 (5′-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3′)and S-D-Bact-0785-a-A-21 (5′-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-3′). The 16S rDNA amplicons were subjected to paired-end sequencing on Illumina MiSeq platform using 2 × 250 bp MiSeq Reagent Kit v2 (Illumina® Inc., San Diego, California).\r\n</ol>","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Chua C, Yong S, Gonzalez M, Lavin P, Cheah Y, Tan G, Wong C (2019): V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=soil_bacteria_king_george_island_and_deception_island&v=1.1","AccessConstraints":null,"UDate":"2019-04-10","CDate":"2019-04-08","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-05-23 01:39:24.004480","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":1,"StatusID":1,"DasType":"Metadata","DasTypeID":8,"DasOrigin":"Research: field survey","Progress":"Completed","AccessConstraint":"Attribution (CC BY)","AccConstrEN":"Attribution (CC BY)","AccConstrDisplay":"<a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\"><img alt=\"Creative Commons License\" style=\"border:0px;height:15px;width:80px;vertical-align:middle;\" src=\"https://www.marinespecies.org/aphia/images/cc/by.png\" /></a> This dataset is licensed under a <a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution 4.0 International License</a>.","License":"https://creativecommons.org/licenses/by/4.0/","AccConstrDescription":"This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.","Lineage":null,"AccConID":21,"DOI":"10.15468/spzi1j","GBIF_UUID":"fca3f5da-a6ae-464d-a488-38439546056d"},"dois":null,"spcols":null,"keywords":[{"ThesaurusTerm":"Antarctica, South Shetland I., Deception I.","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"Antarctica, South Shetland I., Deception I.","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Antarctica, South Shetland I., King George I.","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"Antarctica, South Shetland I., King George I.","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Dna sequencing","ThesTypID":4,"ThesType":"CAB Thesaurus","Code":"40176","Description":null,"OrigThesTerm":"Dna sequencing","DutchTerm":null,"URI":"https://id.cabi.org/cabt/40176","DasKeywordDescr":null},{"ThesaurusTerm":"Metadata","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus","Code":null,"Description":null,"OrigThesTerm":"Metadata","DutchTerm":null,"URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":[{"DasID":1543,"Acronym":"AntOBIS","StandardTitle":"Antarctic Ocean Biodiversity Information System","Relation":"Published dataset","RelationY":"Published in","RelID":13,"doi":null,"vlizDoi":null},{"DasID":5816,"Acronym":"RAS","StandardTitle":"Register of Antarctic Species","Relation":"Partial Source DataSet","RelationY":"Partly included in","RelID":8,"doi":null,"vlizDoi":null}],"ownerships":[{"OrderNr":1,"Surname":"Chua","Firstname":"C.","Initials":"C.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":5,"PersID":37586,"InsID":14324,"FullInstitute":"Universiti Malaysia Sabah","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Universiti Malaysia Sabah","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":2,"Surname":"Yong","Firstname":"S.","Initials":"S.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":5,"PersID":37587,"InsID":14324,"FullInstitute":"Universiti Malaysia Sabah","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Universiti Malaysia Sabah","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":3,"Surname":"Gonzalez","Firstname":"M.","Initials":"M.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"INACH","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":2,"PersID":37588,"InsID":14055,"FullInstitute":"Instituto Antártico Chileno","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Instituto Antártico Chileno","FullAcronym":"INACH","ORC":null,"ROR":null},{"OrderNr":4,"Surname":"Lavin","Firstname":"P.","Initials":"P.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"INACH","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":2,"PersID":37589,"InsID":14055,"FullInstitute":"Instituto Antártico Chileno","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Instituto Antártico Chileno","FullAcronym":"INACH","ORC":null,"ROR":null},{"OrderNr":5,"Surname":"Cheah","Firstname":"Y.","Initials":"Y.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":"ms","OrigNameLangID":40,"FullOrigName":"Universiti Putra Malaysia","InsOwnerCNT":1,"PersID":37590,"InsID":14325,"FullInstitute":"University Putra Malaysia","RoleID":61,"Role":"Data creator","OrigName":"Universiti Putra Malaysia","StandardName":"University Putra Malaysia","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":6,"Surname":"Tan","Firstname":"G.","Initials":"G.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":"ms","OrigNameLangID":40,"FullOrigName":"Universiti Malaya","InsOwnerCNT":1,"PersID":37591,"InsID":14326,"FullInstitute":"University of Malaya","RoleID":61,"Role":"Data creator","OrigName":"Universiti Malaya","StandardName":"University of Malaya","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":7,"Surname":"Wong","Firstname":"C.","Initials":"C.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":5,"PersID":37592,"InsID":14324,"FullInstitute":"Universiti Malaysia Sabah","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Universiti Malaysia Sabah","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":8,"Surname":"Chua","Firstname":"C.","Initials":"C.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":5,"PersID":37586,"InsID":14324,"FullInstitute":"Universiti Malaysia Sabah","RoleID":24,"Role":"Contact","OrigName":null,"StandardName":"Universiti Malaysia Sabah","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":9,"Surname":"Wong","Firstname":"C.","Initials":"C.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":5,"PersID":37592,"InsID":14324,"FullInstitute":"Universiti Malaysia Sabah","RoleID":24,"Role":"Contact","OrigName":null,"StandardName":"Universiti Malaysia Sabah","FullAcronym":null,"ORC":null,"ROR":null}],"taxterms":[{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":11315,"TaxTerm":"Bacteria","AphiaID":6,"DutchTerm":null}],"frameworks":null,"otherterms":null,"temporal":[{"DasDateID":5013,"StartYear":2007,"EndYear":2007,"StartDay":null,"EndDay":null,"StartDate":"2007-02-01","EndDate":"2007-02-01","DasDate":null,"Resolution":null,"ResolutionNL":null,"Notes":null,"StartMonth0":2,"StartMonth":"February","StartMonthNL":"Februari","EndMonth0":2,"EndMonth":"February","EndMonthNL":"Februari","Progress":"Completed","ProgressNL":"Afgelopen"}],"geographical":[{"GeoTerm":"Deception Island","DasGeoID":10417,"DasGeoTerm":null,"DasID":6267,"GeotID":540,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":null,"Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":10341,"OrigGeoTerm":"Deception Island","DutchTerm":null},{"GeoTerm":"King George Island","DasGeoID":10416,"DasGeoTerm":null,"DasID":6267,"GeotID":541,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":null,"Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":12830,"OrigGeoTerm":"King George Island","DutchTerm":null}],"meastypes":[{"MethID":null,"DasMeasTypID":12844,"DasMeasType":"","Matrix":null,"MatrixNL":null,"Parameter":"Molecular data","ParameterNL":"Moleculaire data","Description":null,"Unit":null,"Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null}],"dasthemes":null,"projects":null,"refs":null,"urls":[{"URL":"https://doi.org/10.15468/spzi1j","externalID":"10.15468/spzi1j","URLTypeCode":"DOI","URLType":"DOI","URLTypID":13,"downloadURL":null,"FileName":null},{"URL":"https://www.gbif.org/dataset/fca3f5da-a6ae-464d-a488-38439546056d","externalID":"fca3f5da-a6ae-464d-a488-38439546056d","URLTypeCode":"GBIF_UUID","URLType":"GBIF dataset","URLTypID":43,"downloadURL":null,"FileName":null},{"URL":"https://ipt.biodiversity.aq/resource?r=soil_bacteria_king_george_island_and_deception_island","externalID":null,"URLTypeCode":null,"URLType":"IPT","URLTypID":33,"downloadURL":null,"FileName":null},{"URL":"https://obis.org/dataset/47967e6b-b51d-4337-88f4-bfeaebeff49f","externalID":"47967e6b-b51d-4337-88f4-bfeaebeff49f","URLTypeCode":null,"URLType":"Online dataset","URLTypID":19,"downloadURL":null,"FileName":null}],"pictures":null,"urlmaps":null,"spatreps":null,"fileformats":null,"resmessage":"","complete":1}