{"datasetrec":{"DasID":6695,"Acronym":null,"StandardTitle":"Toxicity of cryoprotectants on early developmental stages of blue mussel, <i>Mytilus galloprovincialis</i>","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":null,"ContactEmail":null,"VersionDate":null,"VersionDay":null,"VersionMonth":null,"VersionYear":null,"SizeReference":null,"EngAbstract":"This dataset is part of the package of data linked to the cryopreservation protocols developed by JRA2 of ASSEMBLE Plus. The data (excel spreadsheet) are the measurements made during crypopreservation tests: the experimental results of toxicity tests of cryoprotecting agents for Blue mussel, <i>Mytilus galloprovincialis</i>, on early developmental stages.","EngDescr":"Mature individuals deposited in PVC tanks with Filtered Sea Water (FSW, 35-37 promille). Cyclic changes of water temperature, from 14°C to 20°C to induce spawning. Spawning individuals put into separate plastic containers to collect sperm and oocytes. After fertilization, incubation of fertilized eggs at a density of 40 individuals/mL. Periodical sampling to collect fertilized egg, trochophore larval stage (18-20h post-fertilization) and 48 and 72h-old D-larval stage for experiments. Cryoprotecting agent solutions prepared at twice the final concentration for the experiments. For toxicity tests, preparation of increasing concentrations (from 1 to 6 M) of Ethylene-Glycol (EG), Propylene-Glycol (PG), Dimethyl-Sulfoxide (Me2SO) and Glycerol (GLY) prepared in FSW. For cryopreservation experiments, 10% (v/v) EG + 0.4M (w/v) trehalose (TRE). Three replicates were assayed for each CPA concentration and control trials. Cryoprotectant solutions added 1:1 to a FSW mL with 400-600 clam individuals in one step. 15 minutes of exposure at room temperature (18-20°C). Samples diluted with FSW 1:1. Then, incubation in 20 mL of clean FSW at 18°C until D-larval stage was reached for exposed fertilized eggs and trochophores, 48h of incubation in the case of D-larval stage. After that, cells fixed with formalin for normal and abnormal D-larval counts. Obtention of normal and abnormal larval precentages, then arcsin-square transformation of data. For toxicity results, statistical analysis following ANOVA-one-way and calculations of NOEC and LOEC levels by post-hoc Dunnet test. For cryopreservation experiments, Bonferroni post-hoc test, assuming homogeneity of variances.","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Heres, P.; Rodriguez-Riveiro, R.; Troncoso, J.; Paredes, E.; Grupo de Investigación en Ecología Costera: Uvigo-EcoCost: Spain; (2021): Toxicity of cryoprotectants on early developmental stages of blue mussel, Mytilus galloprovincialis. Marine Data Archive.","AccessConstraints":null,"UDate":"2022-10-18","CDate":"2021-04-29","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-05-30 01:37:59.451226","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"StatusID":1,"DasType":"Data","DasTypeID":1,"DasOrigin":"Research: lab experiment","Progress":"Completed","AccessConstraint":"Attribution (CC BY)","AccConstrEN":"Attribution (CC BY)","AccConstrDisplay":"<a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\"><img alt=\"Creative Commons License\" style=\"border:0px;height:15px;width:80px;vertical-align:middle;\" src=\"https://www.marinespecies.org/aphia/images/cc/by.png\" /></a> This dataset is licensed under a <a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution 4.0 International License</a>.","License":"https://creativecommons.org/licenses/by/4.0/","AccConstrDescription":"This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.","Lineage":null,"AccConID":21},"dois":null,"spcols":null,"keywords":[{"ThesaurusTerm":"ASSEMBLEPlus Joint Research Activity 2","ThesTypID":8,"ThesType":"Assemble KO Types","Code":"JRA2","Description":null,"OrigThesTerm":"ASSEMBLEPlus Joint Research Activity 2","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Cryopreservation","ThesTypID":4,"ThesType":"CAB Thesaurus","Code":"34188","Description":null,"OrigThesTerm":"Cryopreservation","DutchTerm":null,"URI":"https://id.cabi.org/cabt/34188","DasKeywordDescr":null},{"ThesaurusTerm":"Cryoprotecting agents","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Cryoprotecting agents","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Data","ThesTypID":8,"ThesType":"Assemble KO Types","Code":null,"Description":null,"OrigThesTerm":"Data","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Experimental research","ThesTypID":1,"ThesType":"ASFA Thesaurus List","Code":null,"Description":null,"OrigThesTerm":"Experimental research","DutchTerm":"Experimenteel onderzoek","URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Larval development","ThesTypID":1,"ThesType":"ASFA Thesaurus List","Code":null,"Description":null,"OrigThesTerm":"Larval development","DutchTerm":"Larvale ontwikkeling","URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Marine invertebrates","ThesTypID":1,"ThesType":"ASFA Thesaurus List","Code":"c_4672a815","Description":null,"OrigThesTerm":"Marine invertebrates","DutchTerm":"Mariene invertebraten","URI":"http://aims.fao.org/aos/agrovoc/c_4672a815","DasKeywordDescr":null},{"ThesaurusTerm":"Marine molluscs","ThesTypID":1,"ThesType":"ASFA Thesaurus List","Code":"c_640f05f5","Description":null,"OrigThesTerm":"Marine molluscs","DutchTerm":"Mariene weekdieren","URI":"http://aims.fao.org/aos/agrovoc/c_640f05f5","DasKeywordDescr":null},{"ThesaurusTerm":"Toxicants","ThesTypID":1,"ThesType":"ASFA Thesaurus List","Code":null,"Description":null,"OrigThesTerm":"Toxicants","DutchTerm":"Giftige stoffen","URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":null,"ownerships":[{"OrderNr":2,"Surname":"Paredes Rosendo","Firstname":"Estefanía","Initials":"E.","PerPublicFlag":1,"AdrID":164304,"Email":"eparedes@uvigo.es","InsPublicFlag":1,"Acronym":"EcoCost","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":7,"PersID":40565,"InsID":14840,"FullInstitute":"University of Vigo; 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Toxicity tests of cryoprotecting agents for <i>Mytilus galloprovincialis</i> (Lamark, 1819) early developmental stages. <i>Cryobiology 86</i>: 40-46. <a href=\"https://hdl.handle.net/10.1016/j.cryobiol.2019.01.001\" target=\"_blank\">https://hdl.handle.net/10.1016/j.cryobiol.2019.01.001</a>","RR":"<b>Heres, P. <i>et al.</i></b> (2019). Toxicity tests of cryoprotecting agents for <i>Mytilus galloprovincialis</i> (Lamark, 1819) early developmental stages. <i>Cryobiology 86</i>: 40-46. <a href=\"https://hdl.handle.net/10.1016/j.cryobiol.2019.01.001\" target=\"_blank\">https://hdl.handle.net/10.1016/j.cryobiol.2019.01.001</a>","4":"Toxicity tests of cryoprotecting agents for <i>Mytilus galloprovincialis</i> (Lamark, 1819) early developmental stages","StT":"Toxicity tests of cryoprotecting agents for <i>Mytilus galloprovincialis</i> (Lamark, 1819) early developmental stages","5":"Heres, P.; Rodriguez-Riveiro, R.; Troncoso, J.; Paredes, E.","RSA":"Heres, P.; Rodriguez-Riveiro, R.; Troncoso, J.; Paredes, E.","6":"Gebaseerd op deze dataset","DutchTerm":"Gebaseerd op deze dataset","7":2019,"AnaDate":2019,"8":null,"MonDate":null,"9":". <i>Cryobiology 86</i>: 40-46. <a href=\"https://dx.doi.org/10.1016/j.cryobiol.2019.01.001\" target=\"_blank\">https://dx.doi.org/10.1016/j.cryobiol.2019.01.001</a>","":". <i>Cryobiology 86</i>: 40-46. <a href=\"https://dx.doi.org/10.1016/j.cryobiol.2019.01.001\" target=\"_blank\">https://dx.doi.org/10.1016/j.cryobiol.2019.01.001</a>","10":"https://dx.doi.org/10.1016/j.cryobiol.2019.01.001","doi":"https://dx.doi.org/10.1016/j.cryobiol.2019.01.001"}],"urls":[{"URL":"https://mda.vliz.be/directlink.php?fid=VLIZ_00000858_631ae265dd2d2208170370","externalID":"VLIZ_00000858_631ae265dd2d2208170370","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000858_631ae265dd2d2208170370","FileName":"Heres et al Toxicity of cryoprotectants on early developmental stages of blue mussel Mytilus galloprovincialis.xlsx","Extension":"xlsx"}],"pictures":null,"urlmaps":null,"spatreps":null,"fileformats":null,"resmessage":"","complete":1}