{"datasetrec":{"DasID":7758,"Acronym":null,"StandardTitle":"Bacterioplankon from lakes on Byers Peninsula (2008)","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":"1.0","ContactEmail":null,"VersionDate":null,"VersionDay":16,"VersionMonth":6,"VersionYear":2021,"SizeReference":"0 records (metadata only)","EngAbstract":"Amplicon sequencing dataset (Illumina MiSeq) targeting Bacteria (16S ssu rRNA) in samples (n=9) taken in 2008 from Byers Peninsula (Antarctica).","EngDescr":"Samples in all lakes were obtained from surface depths (0.5–1 m), and approximately in the center of the lake. Samples were then filtered with a vacuum system onto 0.2 μm polycarbonate filters (Nucleopore, Whatman) and stored frozen (−20°C) in Allprotect Tissue Reagent (QIAGEN).\r\n<br>\r\n\tWater samples (n=9) were collected during January and February 2008, in lakes Chester Cone, Midge Lake, Escondido, Limnopolar, Turbio, Somero, and Refugio (Beyers Peninsula, Antarctica)\r\n<br>\r\n\tThe extracted and amplified DNA pool was subjected to an analysis with a Qubit dsDNA HS, Agilent 4200 TapeStation High Sensitivity DNA and Kapa Illumina Library Quantification qPCR assays.\r\n<br>\r\nDNA extraction from each filter was performed with the EZNA Soil DNA isolation kit (Omega Bio-Tek, Inc., Norcross, GA, United States) following the instructions given by the supplier. Sequencing of the region V4 of the 16S rRNA gene was done using the Illumina MiSeq system (2×250 bp) at the genomics facilities of the Research Technology Support Facility of the Michigan State University, United States. For each sample, Illumina compatible, dual indexed amplicon libraries of the 16S-V4 rRNA hypervariable region were created with primers 515f/806r. PCR reactions are composed of 5 μL of 4 μM equimolar primer set, 0.15 μL of AccuPrime Taq DNA High Fidelity Polymerase, 2 μL of 10× AccuPrime PCR Buffer II (Thermo Fisher Scientific, catalog no. 12346094), 11.85 μL of PCR-grade water, and 1 μL of DNA template. The PCR conditions used consisted of 2 min at 95°C, followed by 30 cycles of 95°C for 20 s, 55°C for 15 s, and 72°C for 5 min, followed by 72°C for 10 min.\r\n<br>\r\nCompleted libraries were batch normalized using Invitrogen SequalPrep DNA Normalization Plates. The DNA was sequenced on an 2x250bp Illumina MiSeq v2 flow cell using a MiSeq v2 500 cycle reagent cartridge.","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Picazo A, Rochera C, Villaescusa J A, Javier Miralles-Lorenzo J, Velazquez D, Quesada A, Camacho A (2021): Bacterioplankon from lakes on Byers Peninsula (2008). v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=bacterioplankon_byers_peninsula_lakes_2008&v=1.0","AccessConstraints":"The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.","UDate":"2021-07-05","CDate":"2021-07-05","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-05-14 01:44:00.153333","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":0,"FreshFlag":1,"BrackishFlag":0,"TerrestrialFlag":0,"StatusID":1,"DasType":"Metadata","DasTypeID":8,"DasOrigin":"Research: field survey","Progress":"Completed","AccessConstraint":"Attribution (CC BY)","AccConstrEN":"Attribution (CC BY)","AccConstrDisplay":"<a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\"><img alt=\"Creative Commons License\" style=\"border:0px;height:15px;width:80px;vertical-align:middle;\" src=\"https://www.marinespecies.org/aphia/images/cc/by.png\" /></a> This dataset is licensed under a <a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution 4.0 International License</a>.","License":"https://creativecommons.org/licenses/by/4.0/","AccConstrDescription":"This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.","Lineage":null,"AccConID":21,"DOI":"10.15468/se3ynn","GBIF_UUID":"625b1148-c88d-459b-b3e7-d7504cc6d97d"},"dois":null,"spcols":null,"keywords":[{"ThesaurusTerm":"Antarctica","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"Antarctica","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Metadata","ThesTypID":3,"ThesType":"Transportation Research Thesaurus","Code":"Xbkgdym","Description":null,"OrigThesTerm":"Metadata","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Rrna","ThesTypID":4,"ThesType":"CAB Thesaurus","Code":null,"Description":null,"OrigThesTerm":"Rrna","DutchTerm":null,"URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":[{"DasID":1543,"Acronym":"AntOBIS","StandardTitle":"Antarctic Ocean Biodiversity Information System","Relation":"Published dataset","RelationY":"Published in","RelID":13,"doi":null,"vlizDoi":null},{"DasID":5816,"Acronym":"RAS","StandardTitle":"Register of Antarctic Species","Relation":"Partial Source DataSet","RelationY":"Partly included in","RelID":8,"doi":null,"vlizDoi":null}],"ownerships":[{"OrderNr":1,"Surname":"Camacho","Firstname":"Antonio","Initials":"A.","PerPublicFlag":1,"AdrID":164117,"Email":"antonio.camacho@uv.es","InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":2,"PersID":40351,"InsID":3116,"FullInstitute":"University of Valencia","RoleID":24,"Role":"Contact","OrigName":null,"StandardName":"University of Valencia","FullAcronym":null,"ORC":null,"ROR":null},{"OrderNr":2,"Surname":"Camacho","Firstname":"Antonio","Initials":"A.","PerPublicFlag":1,"AdrID":164117,"Email":"antonio.camacho@uv.es","InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":2,"PersID":40351,"InsID":3116,"FullInstitute":"University of Valencia","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"University of Valencia","FullAcronym":null,"ORC":null,"ROR":null}],"taxterms":[{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":11315,"TaxTerm":"Bacteria","AphiaID":6,"DutchTerm":null}],"frameworks":null,"otherterms":null,"temporal":[{"DasDateID":5466,"StartYear":2008,"EndYear":2008,"StartDay":28,"EndDay":8,"StartDate":"2008-01-28","EndDate":"2008-02-08","DasDate":null,"Resolution":null,"ResolutionNL":null,"Notes":null,"StartMonth0":1,"StartMonth":"January","StartMonthNL":"Januari","EndMonth0":2,"EndMonth":"February","EndMonthNL":"Februari","Progress":"Completed","ProgressNL":"Afgelopen"}],"geographical":[{"GeoTerm":"Antarctica","DasGeoID":11730,"DasGeoTerm":null,"DasID":7758,"GeotID":496,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":"Lakes from Beyers Peninsula","Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":1926,"OrigGeoTerm":"Antarctica","DutchTerm":"Antarctica"}],"meastypes":[{"MethID":null,"DasMeasTypID":13897,"DasMeasType":"","Matrix":null,"MatrixNL":null,"Parameter":"Occurrence of biota","ParameterNL":"Voorkomen van soorten","Description":null,"Unit":null,"Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null}],"dasthemes":[{"DasThemeID":30,"DasTheme":"Biology > Ecology - biodiversity","DasTheme0":"Ecology - biodiversity","DutchTerm":"Ecologie - biodiversiteit"},{"DasThemeID":87,"DasTheme":"Biology > Plankton > Bacterioplankton","DasTheme0":"Bacterioplankton","DutchTerm":"Bacterioplankton"}],"projects":null,"refs":null,"urls":[{"URL":"https://doi.org/10.15468/se3ynn","externalID":"10.15468/se3ynn","URLTypeCode":"DOI","URLType":"DOI","URLTypID":13,"downloadURL":null,"FileName":null},{"URL":"https://www.gbif.org/dataset/625b1148-c88d-459b-b3e7-d7504cc6d97d","externalID":"625b1148-c88d-459b-b3e7-d7504cc6d97d","URLTypeCode":"GBIF_UUID","URLType":"GBIF dataset","URLTypID":43,"downloadURL":null,"FileName":null},{"URL":"https://ipt.biodiversity.aq/resource?r=bacterioplankon_byers_peninsula_lakes_2008","externalID":null,"URLTypeCode":null,"URLType":"IPT","URLTypID":33,"downloadURL":null,"FileName":null},{"URL":"https://obis.org/dataset/44ee228b-0759-442f-a04f-ce5c6664820a","externalID":"44ee228b-0759-442f-a04f-ce5c6664820a","URLTypeCode":null,"URLType":"Online dataset","URLTypID":19,"downloadURL":null,"FileName":null}],"pictures":null,"urlmaps":null,"spatreps":null,"fileformats":null,"resmessage":"","complete":1}