{"datasetrec":{"DasID":7760,"Acronym":null,"StandardTitle":"Carotenoid producing bacteria from Antarctica","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":"1.0","ContactEmail":null,"VersionDate":null,"VersionDay":15,"VersionMonth":6,"VersionYear":2021,"SizeReference":"30 records","EngAbstract":"A dataset of 30 bacterial cultures from coastal and marine areas in 2014 from sites in Fildes Peninsula, King George Island (Antarctica). Identification based on 16S ssu rRNA sequencing.","EngDescr":"A total of 32 liquid and solid samples were collected in 50 mL sterile tubes.\r\n<br>\r\n\tEnvironmental samples collected from Fildes Peninsula, King George Island, during the expedition organized by IAU (Uruguayan Antarctic Institute) on December 2014.\r\n<br>\r\nApproximately 100 mg of each sample was suspended in 900 μl of sterile NaCl 0.9% (w/v) solution, serially diluted and plated on adequate media. The isolation medium for samples of organic matter, sediments and ice water was Tryptic Soy Agar (TSA, Sigma Aldrich), and for sea water was TSA complemented with 20 g/L of sea salts (Sigma). Plates were incubated at 10 °C for 7–10 days, and colored colonies were selected for strain isolation by streak-plating technique. Once purity was verified, strains were conserved at −80 °C on glass beads with 20% glycerol in Tryptic Soy Broth (TSB, Oxoid) and sea salts when needed. Strains were characterized by colony and cell morphology, Gram staining and pigment composition.\r\n<br>\r\nGenomic DNA extraction was performed with a commercial kit according manufacturer’s instructions (Genomic DNA Purification Kit, Thermo Fisher). Amplification of the 16S rRNA gene fragments were done in a Palm-1870 Cycler TM (Corbett Research UK Ltd) as follows: initial denaturation 3 min at 95 °C, then 35 cycles of 45 s at 94 °C, 45 s at 58 °C, 60 s 72 °C, and a final extension step 9 min at 72 °C. Reaction mixtures contained: 2.5 U polymerase (Mango Taq, Bioline), 10 μL of buffer solution, 2.5 μL of 50 mM MgCl2, and 2.5 μL each of forward primer 27 F (5´-AGAGTTTGATC MTGGCTCAG-3´) and reverse primer 1492R (5´-TACGGYTACC TTGTTACGACTT-3´), genomic DNA, and water to 50 μL final volume. The PCR products were analyzed by electrophoresis with 1% agarose gels. DNA sequencing was carried out by Macrogen Inc. (Korea) using universal primers.","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Vila E, Hornero-Mendez D, Azziz G, Lareo C, Saravia V (2021): Carotenoid producing bacteria from Antarctica. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Occurrence. https://ipt.biodiversity.aq/resource?r=carotenoid_bacteria_antarctica&v=1.0","AccessConstraints":"The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.","UDate":"2021-07-05","CDate":"2021-07-05","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-06-05 01:37:23.644310","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"StatusID":1,"DasType":"Data","DasTypeID":1,"DasOrigin":"Research: field survey","Progress":"Completed","AccessConstraint":"Attribution (CC BY)","AccConstrEN":"Attribution (CC BY)","AccConstrDisplay":"<a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\"><img alt=\"Creative Commons License\" style=\"border:0px;height:15px;width:80px;vertical-align:middle;\" src=\"https://www.marinespecies.org/aphia/images/cc/by.png\" /></a> This dataset is licensed under a <a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution 4.0 International License</a>.","License":"https://creativecommons.org/licenses/by/4.0/","AccConstrDescription":"This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.","Lineage":null,"AccConID":21,"DOI":"10.15468/cur76e","GBIF_UUID":"d5010a6c-152b-4565-b799-cc8eb0e7a77b"},"dois":null,"spcols":null,"keywords":[{"ThesaurusTerm":"Coastal","ThesTypID":4,"ThesType":"CAB Thesaurus","Code":null,"Description":null,"OrigThesTerm":"Coastal","DutchTerm":"Kustgebonden","URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Cultures","ThesTypID":1,"ThesType":"ASFA Thesaurus List","Code":null,"Description":null,"OrigThesTerm":"Cultures","DutchTerm":"Culturen","URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"PSW, Antarctica, South Shetland I., King George I.","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"PSW, Antarctica, South Shetland I., King George I.","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Rrna","ThesTypID":4,"ThesType":"CAB Thesaurus","Code":null,"Description":null,"OrigThesTerm":"Rrna","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Sequencing","ThesTypID":3,"ThesType":"Transportation Research Thesaurus","Code":"Cpks","Description":null,"OrigThesTerm":"Sequencing","DutchTerm":null,"URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":[{"DasID":1543,"Acronym":"AntOBIS","StandardTitle":"Antarctic Ocean Biodiversity Information System","Relation":"Published dataset","RelationY":"Published in","RelID":13,"doi":null,"vlizDoi":null},{"DasID":5816,"Acronym":"RAS","StandardTitle":"Register of Antarctic Species","Relation":"Partial Source DataSet","RelationY":"Partly included in","RelID":8,"doi":null,"vlizDoi":null}],"ownerships":[{"OrderNr":1,"Surname":"Saravia","Firstname":"Veronica","Initials":null,"PerPublicFlag":1,"AdrID":164120,"Email":"vsaravia@fing.edu.uy","InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":2,"PersID":40353,"InsID":14762,"FullInstitute":"University of the Republic of Uruguay; 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