{"datasetrec":{"DasID":7900,"Acronym":null,"StandardTitle":"Data of a long-term study on survival and development of successive generations of <i>Mytilus galloprovincialis</i> cryopreserved larvae","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":null,"ContactEmail":null,"VersionDate":null,"VersionDay":null,"VersionMonth":null,"VersionYear":null,"SizeReference":null,"EngAbstract":"This dataset is part of the package of data linked to the cryopreservation protocols developed by JRA2 of ASSEMBLE Plus.  The aim of this work was to study the improvement of cryopreservation protocol for <i>Mytilus galloprovincialis</i> larvae described in Paredes et al., 2021 studying different development stages, toxic and cryoprotection effects of Cryoprotecting Agent (CPAs), cooling and thawing rates and the use of sucrose (SUC) for CPA removal. Moreover, the capability of producing adult mussels from cryopreserved 72h-old D-larvae and potential long-term effects of cryopreservation through progenies was evaluated. The data (excel spreadsheet) are the measurements made during these crypopreservation tests.","EngDescr":"Shellfish aquaculture needs the development of new tools for the improvement of good practices avoiding the reliance of natural spat collection leading to an increase of production more efficiently.\r\nResults showed that the selection of 72 h-old D-larvae for cryopreservation yielded around 75% of recovery, higher than 50% from trochophores. 10% Ethylene-Glycol + 0.4 M Trehalose in Filtered Sea Water (FSW) was the best CPA combination for D-larvae, as well as cooling at -1°C/min and water bath at 35 °C for thawing. SUC solutions did not improved larval recovery at any of the concentrations tested (p>0.05). At settlement, 5.26% of cryopreserved F1 larvae survived and over 70% were able to settle. F2 cryopreservation produced 0.15% of spat and settlement success varied from 35 to 50%. The delay of shell size showed on cryopreserved larvae was in declining throughout larval rearing without significant differences with controls from settlement point (p>0.05). Long-term experiments showed that it is possible to obtain adult mussels from cryopreserved larvae and this tool does not compromise the quality of following progenies, neither cryopreservation nor post-thawing development of them.","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Heres, P.; Troncoso, J.; Paredes, E.; Grupo de Investigación en Ecología Costera (Uvigo-EcoCost): Spain; (2021); Data of a long-term study on survival and development of successive generations of <i>Mytilus galloprovincialis</i> cryopreserved larvae. Marine Data Archive.","AccessConstraints":null,"UDate":"2022-10-18","CDate":"2021-10-18","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-05-14 01:44:00.153333","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"StatusID":1,"DasType":"Data","DasTypeID":1,"DasOrigin":"Research: lab experiment","Progress":"Completed","AccessConstraint":"Attribution (CC BY)","AccConstrEN":"Attribution (CC BY)","AccConstrDisplay":"<a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\"><img alt=\"Creative Commons License\" style=\"border:0px;height:15px;width:80px;vertical-align:middle;\" src=\"https://www.marinespecies.org/aphia/images/cc/by.png\" /></a> This dataset is licensed under a <a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution 4.0 International License</a>.","License":"https://creativecommons.org/licenses/by/4.0/","AccConstrDescription":"This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.","Lineage":null,"AccConID":21},"dois":[{"DOIID":584,"PublicationYear":2021,"DOI":"10.14284/526","Citation":"Heres, P.; Troncoso, J.; Paredes, E.; Grupo de Investigación en Ecología Costera (Uvigo-EcoCost): Spain; (2021); Data of a long-term study on survival and development of successive generations of Mytilus galloprovincialis cryopreserved larvae. Marine Data Archive.","MDAID":"VLIZ_00000311_617bdd1e42e90469481787","Moratorium":0,"MDAURL":"https://mda.vliz.be/directlink.php?fid=VLIZ_00000311_617bdd1e42e90469481787","CurrentDOI":1,"Active":1,"URL":null,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000311_617bdd1e42e90469481787"}],"spcols":null,"keywords":[{"ThesaurusTerm":"ASSEMBLEPlus Joint Research Activity 2","ThesTypID":8,"ThesType":"Assemble KO Types","Code":"JRA2","Description":null,"OrigThesTerm":"ASSEMBLEPlus Joint Research Activity 2","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Cryopreservation","ThesTypID":4,"ThesType":"CAB Thesaurus","Code":"34188","Description":null,"OrigThesTerm":"Cryopreservation","DutchTerm":null,"URI":"https://id.cabi.org/cabt/34188","DasKeywordDescr":null},{"ThesaurusTerm":"Cryoprotecting agents","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Cryoprotecting agents","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Data","ThesTypID":8,"ThesType":"Assemble KO Types","Code":null,"Description":null,"OrigThesTerm":"Data","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"D-larvae","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"D-larvae","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Larval rearing","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Larval rearing","DutchTerm":null,"URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":null,"ownerships":[{"OrderNr":1,"Surname":"Paredes Rosendo","Firstname":"Estefanía","Initials":"E.","PerPublicFlag":1,"AdrID":164304,"Email":"eparedes@uvigo.es","InsPublicFlag":1,"Acronym":"EcoCost","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":5,"PersID":40565,"InsID":14840,"FullInstitute":"University of Vigo; Ecology and Animal Biology Department; Facultad de Ciencias do Mar; Grupo de Investigación en Ecología Costera","RoleID":24,"Role":"Contact","OrigName":null,"StandardName":"Grupo de Investigación en Ecología Costera","FullAcronym":"Uvigo-EcoCost","ORC":"https://orcid.org/0000-0001-8829-360X","ROR":null},{"OrderNr":2,"Surname":"Heres Gozalbes","Firstname":"Pablo","Initials":"P.","PerPublicFlag":1,"AdrID":164301,"Email":"pheres@uvigo.es","InsPublicFlag":1,"Acronym":"EcoCost","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":5,"PersID":40562,"InsID":14840,"FullInstitute":"University of Vigo; Ecology and Animal Biology Department; Facultad de Ciencias do Mar; Grupo de Investigación en Ecología Costera","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Grupo de Investigación en Ecología Costera","FullAcronym":"Uvigo-EcoCost","ORC":null,"ROR":null},{"OrderNr":3,"Surname":"Souza Troncoso","Firstname":"Jesús","Initials":"J.","PerPublicFlag":1,"AdrID":164305,"Email":"troncoso@uvigo.es","InsPublicFlag":1,"Acronym":"EcoCost","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":5,"PersID":24240,"InsID":14840,"FullInstitute":"University of Vigo; Ecology and Animal Biology Department; Facultad de Ciencias do Mar; Grupo de Investigación en Ecología Costera","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Grupo de Investigación en Ecología Costera","FullAcronym":"Uvigo-EcoCost","ORC":"https://orcid.org/0000-0002-7305-6879","ROR":null},{"OrderNr":4,"Surname":"Paredes Rosendo","Firstname":"Estefanía","Initials":"E.","PerPublicFlag":1,"AdrID":164304,"Email":"eparedes@uvigo.es","InsPublicFlag":1,"Acronym":"EcoCost","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":5,"PersID":40565,"InsID":14840,"FullInstitute":"University of Vigo; Ecology and Animal Biology Department; Facultad de Ciencias do Mar; Grupo de Investigación en Ecología Costera","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Grupo de Investigación en Ecología Costera","FullAcronym":"Uvigo-EcoCost","ORC":"https://orcid.org/0000-0001-8829-360X","ROR":null},{"OrderNr":5,"Surname":null,"Firstname":null,"Initials":null,"PerPublicFlag":null,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"EcoCost","OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":5,"PersID":null,"InsID":14840,"FullInstitute":"University of Vigo; Ecology and Animal Biology Department; Facultad de Ciencias do Mar; Grupo de Investigación en Ecología Costera","RoleID":61,"Role":"Data creator","OrigName":null,"StandardName":"Grupo de Investigación en Ecología Costera","FullAcronym":"Uvigo-EcoCost","ORC":null,"ROR":null}],"taxterms":[{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":18296,"TaxTerm":"Mytilus galloprovincialis Lamarck, 1819","AphiaID":140481,"DutchTerm":"Diepwatermossel"}],"frameworks":null,"otherterms":[{"OtherTerm":"Cryoprotecting agents"},{"OtherTerm":"D-larvae"},{"OtherTerm":"Larval rearing"}],"temporal":[{"DasDateID":5592,"StartYear":2018,"EndYear":2021,"StartDay":9,"EndDay":5,"StartDate":"2018-10-09","EndDate":"2021-04-05","DasDate":null,"Resolution":null,"ResolutionNL":null,"Notes":null,"StartMonth0":10,"StartMonth":"October","StartMonthNL":"Oktober","EndMonth0":4,"EndMonth":"April","EndMonthNL":"April","Progress":"Completed","ProgressNL":"Afgelopen"}],"geographical":null,"meastypes":[{"MethID":null,"DasMeasTypID":14063,"DasMeasType":"","Matrix":null,"MatrixNL":null,"Parameter":"Count of larvae","ParameterNL":null,"Description":null,"Unit":null,"Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null}],"dasthemes":[{"DasThemeID":29,"DasTheme":"Biology > Invertebrates","DasTheme0":"Invertebrates","DutchTerm":"Invertebraten"}],"projects":[{"ProID":4868,"Acronym":"ASSEMBLE+","Progress":"Completed","StandardTitle":"Association of European Marine Biological Laboratories Expanded","FP7Code":null,"GrantDOI":null,"FunderID":"730984","FunderIDType":"EU contract id","FunderCodes":["Horizon Europe"]}],"refs":[{"0":356381,"BRefID":356381,"1":"Based on this dataset","Relation":"Based on this dataset","2":2,"RelationID":2,"3":"<b>Heres, P.; Troncoso, J.; Paredes, E.</b> (2022). Long-term study on survival and development of successive generations of <i>Mytilus galloprovincialis</i> cryopreserved larvae. <i>NPG Scientific Reports 12(1)</i>: 13632. <a href=\"https://dx.doi.org/10.1038/s41598-022-17935-0\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-022-17935-0</a>","RR":"<b>Heres, P.; Troncoso, J.; Paredes, E.</b> (2022). Long-term study on survival and development of successive generations of <i>Mytilus galloprovincialis</i> cryopreserved larvae. <i>NPG Scientific Reports 12(1)</i>: 13632. <a href=\"https://dx.doi.org/10.1038/s41598-022-17935-0\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-022-17935-0</a>","4":"Long-term study on survival and development of successive generations of <i>Mytilus galloprovincialis</i> cryopreserved larvae","StT":"Long-term study on survival and development of successive generations of <i>Mytilus galloprovincialis</i> cryopreserved larvae","5":"Heres, P.; Troncoso, J.; Paredes, E.","RSA":"Heres, P.; Troncoso, J.; Paredes, E.","6":"Gebaseerd op deze dataset","DutchTerm":"Gebaseerd op deze dataset","7":2022,"AnaDate":2022,"8":null,"MonDate":null,"9":". <i>NPG Scientific Reports 12(1)</i>: 13632. <a href=\"https://dx.doi.org/10.1038/s41598-022-17935-0\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-022-17935-0</a>","":". <i>NPG Scientific Reports 12(1)</i>: 13632. <a href=\"https://dx.doi.org/10.1038/s41598-022-17935-0\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-022-17935-0</a>","10":"https://dx.doi.org/10.1038/s41598-022-17935-0","doi":"https://dx.doi.org/10.1038/s41598-022-17935-0"}],"urls":[{"URL":"https://mda.vliz.be/directlink.php?fid=VLIZ_00000858_61f922e3380c7628956541","externalID":"VLIZ_00000858_61f922e3380c7628956541","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000858_61f922e3380c7628956541","FileName":"Heres_et_al Long-term study on survival and development of successive generations of M. galloprovincialis cryopreserved larvae.xlsx","Extension":"xlsx"}],"pictures":null,"urlmaps":null,"spatreps":null,"fileformats":null,"resmessage":"","complete":1}