{"datasetrec":{"DasID":7901,"Acronym":null,"StandardTitle":"Data on long term survival of cryopreserved mussel larvae (<i>Mytilus galloprovinciallis</i>)","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":null,"ContactEmail":null,"VersionDate":null,"VersionDay":null,"VersionMonth":null,"VersionYear":null,"SizeReference":null,"EngAbstract":"This dataset is part of the package of data linked to the cryopreservation protocols developed by JRA2 of ASSEMBLE Plus. The data (excel spreadsheet) are the measurements made during the crypopreservation tests.  The aim of this study was to develop a successful cryopreservation protocol for <i>Mytilus galloprovincialis</i> larvae and study long-term effects by assessing survival and development post cryobanking. ","EngDescr":"Due to the economic importance of the mussel <i>Mytilus galloprovincialis</i> in the Spanish aquaculture, there is a growing interest in developing alternative methods to ensure the supply of good-quality biological material and to minimize the uncertainty and variability that might be a source of economic risk for this sector. We evaluated the effect of previously studied cryoprotecting agent, Ethylene glycol (EG) for cryopreservation of trochophore larvae and D-veliger larvae 48h and 72h old, following an existing preliminary protocol for <i>M. galloprovincialis</i> mussel trochophores. The protocol selected for cryopreservation consisted on holding at 4°C for 2min, then cooling at 1°C/min to −12°C, holding for 5min, then cooling at −35°C with a rate of 1°C/min, then plunging into liquid nitrogen for storage. Thawing took place by immersion in 35°C water bath. With this protocol the initial percentage of short-term developed a D-larvae (48h incubation post-thaw) was 48.9+/-7.6% with 10% EG + 0.2M Trehalose (TRE). This initial test only studied the trochophore larvae until they reached the D-stage (48h old); in the present experiment experiments, we replicated this study but also carried out a long-term larval rearing with cryopreserved trochophore larvae in comparison to fresh larvae. This experiment allowed a comparative post-freezing analysis of both larval development and survival. Larval settlement was also characterized after 13days. The data revealed that until settling, the survival of the cryopreserved larvae was slightly lower than the 2.8% reported in the larval rearing for Greenshell mussel. Over time, there was an initial difference in size of cryopreserved larvae when compared to controls, but from day 17 onwards the size between both types of larvae began to stabilize. Attending to settlement, we obtained a 64% of success of cryopreserved larvae respect to the control.","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Rodriguez-Riveiro, R.; Heres, P.; Troncoso, J.; Paredes, E.; Estación de Ciencias Mariñas de Toralla (Uvigo-ECIMAT): Spain; (2021): Data on long term survival of cryopreserved mussel larvae (<i>Mytilus galloprovinciallis</i>).","AccessConstraints":null,"UDate":"2022-10-18","CDate":"2021-10-18","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-05-14 01:44:00.153333","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"StatusID":1,"DasType":"Data","DasTypeID":1,"DasOrigin":"Research: lab experiment","Progress":"Completed","AccessConstraint":"Attribution (CC BY)","AccConstrEN":"Attribution (CC BY)","AccConstrDisplay":"<a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\"><img alt=\"Creative Commons License\" style=\"border:0px;height:15px;width:80px;vertical-align:middle;\" src=\"https://www.marinespecies.org/aphia/images/cc/by.png\" /></a> This dataset is licensed under a <a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution 4.0 International License</a>.","License":"https://creativecommons.org/licenses/by/4.0/","AccConstrDescription":"This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.","Lineage":null,"AccConID":21},"dois":null,"spcols":null,"keywords":[{"ThesaurusTerm":"ASSEMBLEPlus Joint Research Activity 2","ThesTypID":8,"ThesType":"Assemble KO Types","Code":"JRA2","Description":null,"OrigThesTerm":"ASSEMBLEPlus Joint Research Activity 2","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Cryopreservation","ThesTypID":4,"ThesType":"CAB Thesaurus","Code":"34188","Description":null,"OrigThesTerm":"Cryopreservation","DutchTerm":null,"URI":"https://id.cabi.org/cabt/34188","DasKeywordDescr":null},{"ThesaurusTerm":"Data","ThesTypID":8,"ThesType":"Assemble KO Types","Code":null,"Description":null,"OrigThesTerm":"Data","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Larval rearing","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Larval rearing","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Trochophore larvae","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Trochophore larvae","DutchTerm":null,"URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":null,"ownerships":[{"OrderNr":1,"Surname":"Paredes Rosendo","Firstname":"Estefanía","Initials":"E.","PerPublicFlag":1,"AdrID":167128,"Email":"eparedes@uvigo.es","InsPublicFlag":1,"Acronym":"ECIMAT","OrigNameLangCode":"es","OrigNameLangID":17,"FullOrigName":"Universidad de Vigo; Estacíon de Ciencias Mariñas de Toralla","InsOwnerCNT":6,"PersID":40565,"InsID":10019,"FullInstitute":"University of Vigo; Departamento de Ecología y Biología Animal; Station of marine Science of Toralla","RoleID":24,"Role":"Contact","OrigName":"Estacíon de Ciencias Mariñas de Toralla","StandardName":"Station of marine Science of Toralla","FullAcronym":"Uvigo-ECIMAT","ORC":"https://orcid.org/0000-0001-8829-360X","ROR":null},{"OrderNr":2,"Surname":"Rodríguez Riveiro","Firstname":"Rosalía","Initials":"R.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"ECIMAT","OrigNameLangCode":"es","OrigNameLangID":17,"FullOrigName":"Universidad de Vigo; Estacíon de Ciencias Mariñas de Toralla","InsOwnerCNT":6,"PersID":40563,"InsID":10019,"FullInstitute":"University of Vigo; Departamento de Ecología y Biología Animal; Station of marine Science of Toralla","RoleID":61,"Role":"Data creator","OrigName":"Estacíon de Ciencias Mariñas de Toralla","StandardName":"Station of marine Science of Toralla","FullAcronym":"Uvigo-ECIMAT","ORC":null,"ROR":null},{"OrderNr":3,"Surname":"Heres Gozalbes","Firstname":"Pablo","Initials":"P.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"ECIMAT","OrigNameLangCode":"es","OrigNameLangID":17,"FullOrigName":"Universidad de Vigo; Estacíon de Ciencias Mariñas de Toralla","InsOwnerCNT":6,"PersID":40562,"InsID":10019,"FullInstitute":"University of Vigo; Departamento de Ecología y Biología Animal; Station of marine Science of Toralla","RoleID":61,"Role":"Data creator","OrigName":"Estacíon de Ciencias Mariñas de Toralla","StandardName":"Station of marine Science of Toralla","FullAcronym":"Uvigo-ECIMAT","ORC":null,"ROR":null},{"OrderNr":4,"Surname":"Souza Troncoso","Firstname":"Jesús","Initials":"J.","PerPublicFlag":1,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"ECIMAT","OrigNameLangCode":"es","OrigNameLangID":17,"FullOrigName":"Universidad de Vigo; Estacíon de Ciencias Mariñas de Toralla","InsOwnerCNT":6,"PersID":24240,"InsID":10019,"FullInstitute":"University of Vigo; Departamento de Ecología y Biología Animal; Station of marine Science of Toralla","RoleID":61,"Role":"Data creator","OrigName":"Estacíon de Ciencias Mariñas de Toralla","StandardName":"Station of marine Science of Toralla","FullAcronym":"Uvigo-ECIMAT","ORC":"https://orcid.org/0000-0002-7305-6879","ROR":null},{"OrderNr":5,"Surname":"Paredes Rosendo","Firstname":"Estefanía","Initials":"E.","PerPublicFlag":1,"AdrID":167128,"Email":"eparedes@uvigo.es","InsPublicFlag":1,"Acronym":"ECIMAT","OrigNameLangCode":"es","OrigNameLangID":17,"FullOrigName":"Universidad de Vigo; Estacíon de Ciencias Mariñas de Toralla","InsOwnerCNT":6,"PersID":40565,"InsID":10019,"FullInstitute":"University of Vigo; Departamento de Ecología y Biología Animal; Station of marine Science of Toralla","RoleID":61,"Role":"Data creator","OrigName":"Estacíon de Ciencias Mariñas de Toralla","StandardName":"Station of marine Science of Toralla","FullAcronym":"Uvigo-ECIMAT","ORC":"https://orcid.org/0000-0001-8829-360X","ROR":null},{"OrderNr":6,"Surname":null,"Firstname":null,"Initials":null,"PerPublicFlag":null,"AdrID":null,"Email":null,"InsPublicFlag":1,"Acronym":"ECIMAT","OrigNameLangCode":"es","OrigNameLangID":17,"FullOrigName":"Universidad de Vigo; Estacíon de Ciencias Mariñas de Toralla","InsOwnerCNT":6,"PersID":null,"InsID":10019,"FullInstitute":"University of Vigo; Departamento de Ecología y Biología Animal; Station of marine Science of Toralla","RoleID":61,"Role":"Data creator","OrigName":"Estacíon de Ciencias Mariñas de Toralla","StandardName":"Station of marine Science of Toralla","FullAcronym":"Uvigo-ECIMAT","ORC":null,"ROR":null}],"taxterms":[{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":18296,"TaxTerm":"Mytilus galloprovincialis Lamarck, 1819","AphiaID":140481,"DutchTerm":"Diepwatermossel"}],"frameworks":null,"otherterms":[{"OtherTerm":"Larval rearing"},{"OtherTerm":"Trochophore larvae"}],"temporal":[{"DasDateID":5593,"StartYear":2017,"EndYear":2017,"StartDay":16,"EndDay":7,"StartDate":"2017-11-16","EndDate":"2017-12-07","DasDate":null,"Resolution":null,"ResolutionNL":null,"Notes":null,"StartMonth0":11,"StartMonth":"November","StartMonthNL":"November","EndMonth0":12,"EndMonth":"December","EndMonthNL":"December","Progress":"Completed","ProgressNL":"Afgelopen"}],"geographical":null,"meastypes":[{"MethID":null,"DasMeasTypID":14064,"DasMeasType":"","Matrix":null,"MatrixNL":null,"Parameter":"Count of larvae","ParameterNL":null,"Description":null,"Unit":null,"Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null}],"dasthemes":[{"DasThemeID":29,"DasTheme":"Biology > Invertebrates","DasTheme0":"Invertebrates","DutchTerm":"Invertebraten"}],"projects":[{"ProID":4868,"Acronym":"ASSEMBLE+","Progress":"Completed","StandardTitle":"Association of European Marine Biological Laboratories Expanded","FP7Code":null,"GrantDOI":null,"FunderID":"730984","FunderIDType":"EU contract id","FunderCodes":["Horizon Europe"]}],"refs":[{"0":323710,"BRefID":323710,"1":"Based on this dataset","Relation":"Based on this dataset","2":2,"RelationID":2,"3":"<b>Rodriguez-Riveiro, R. <i>et al.</i></b> (2019). Long term survival of cryopreserved mussel larvae (<i>Mytilus galloprovinciallis</i>). <i>Aquaculture 512</i>: 734326. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2019.734326\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2019.734326</a>","RR":"<b>Rodriguez-Riveiro, R. <i>et al.</i></b> (2019). Long term survival of cryopreserved mussel larvae (<i>Mytilus galloprovinciallis</i>). <i>Aquaculture 512</i>: 734326. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2019.734326\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2019.734326</a>","4":"Long term survival of cryopreserved mussel larvae (<i>Mytilus galloprovinciallis</i>)","StT":"Long term survival of cryopreserved mussel larvae (<i>Mytilus galloprovinciallis</i>)","5":"Rodriguez-Riveiro, R.; Heres, P.; Troncoso, J.; Paredes, E.","RSA":"Rodriguez-Riveiro, R.; Heres, P.; Troncoso, J.; Paredes, E.","6":"Gebaseerd op deze dataset","DutchTerm":"Gebaseerd op deze dataset","7":2019,"AnaDate":2019,"8":null,"MonDate":null,"9":". <i>Aquaculture 512</i>: 734326. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2019.734326\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2019.734326</a>","":". <i>Aquaculture 512</i>: 734326. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2019.734326\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2019.734326</a>","10":"https://dx.doi.org/10.1016/j.aquaculture.2019.734326","doi":"https://dx.doi.org/10.1016/j.aquaculture.2019.734326"}],"urls":[{"URL":"https://mda.vliz.be/directlink.php?fid=VLIZ_00000858_61f924025880b579660997","externalID":"VLIZ_00000858_61f924025880b579660997","URLTypeCode":null,"URLType":"Archived file","URLTypID":28,"downloadURL":"https://mda.vliz.be/download.php?file=VLIZ_00000858_61f924025880b579660997","FileName":"Rodriguez-Riveiro et al. 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