{"datasetrec":{"DasID":8063,"Acronym":null,"StandardTitle":"gENESIS results 2022","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":null,"ContactEmail":"silvia.mercurio@unimi.it","VersionDate":null,"VersionDay":null,"VersionMonth":null,"VersionYear":null,"SizeReference":null,"EngAbstract":"COVID-19 pandemic strongly affected gENESiS, drastically reducing project length. We managed to perform some immunolocalization with rhodamine phalloidin on crinoid embryos and tried out a new in situ hybridization technique called HCR in situ with specific probes for the evolutionary conserved muscle terminal differentiation gene myosin heavy chain (mhc). Using this new approach we did obtain very nice results, with detection through the HCR in situ approach of an expression pattern for mhc than was similar than that classically obtained with the common chromogenic in situ hybridization technique.","EngDescr":null,"OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Mercurio, S.; Pennati, R.; Università degli Studi di Milano: Italy; (2022): gENESIS results 2022.","AccessConstraints":null,"UDate":"2022-05-03","CDate":"2022-05-03","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2026-05-30 01:37:59.451226","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"StatusID":1,"DasType":"Data","DasTypeID":1,"DasOrigin":"Research: lab experiment","Progress":"Completed","AccessConstraint":"Unrestricted after moratorium period","AccConstrEN":"Unrestricted after moratorium period","AccConstrDisplay":null,"License":null,"AccConstrDescription":"Data are initially restricted, but the access condition relaxes to academic or unrestricted once a specified period of time after an event (such as collection, publication, completion of QC procedures or project cessation) has elapsed","Lineage":null,"AccConID":8},"dois":null,"spcols":null,"keywords":[{"ThesaurusTerm":"ANE, France, Mediterranean Sea","ThesTypID":35,"ThesType":"Geoterms no link Marine Regions","Code":null,"Description":null,"OrigThesTerm":"ANE, France, Mediterranean Sea","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Crinoids","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Crinoids","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Sea urchins","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Sea urchins","DutchTerm":null,"URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":null,"ownerships":null,"taxterms":[{"DasTaxtTypID":1,"DasTaxtType":"Taxonomic coverage","TaxtID":15377,"TaxTerm":"Echinodermata","AphiaID":1806,"DutchTerm":"Stekelhuidigen"}],"frameworks":null,"otherterms":[{"OtherTerm":"Crinoids"},{"OtherTerm":"Sea urchins"}],"temporal":null,"geographical":[{"GeoTerm":null,"DasGeoID":13410,"DasGeoTerm":null,"DasID":8063,"GeotID":null,"X":null,"Y":null,"MaxX":null,"MaxY":null,"StationName":null,"Precision":null,"CoordSystID":null,"GeoDatumID":null,"OrigCoordMinX":null,"OrigCoordMinY":null,"OrigCoordMaxX":null,"OrigCoordMaxY":null,"OrderNr":null,"Projection":null,"GeoDatum":null,"GeoObjectID":null,"OrigGeoTerm":null,"DutchTerm":null}],"meastypes":[{"MethID":null,"DasMeasTypID":14319,"DasMeasType":"","Matrix":null,"MatrixNL":null,"Parameter":null,"ParameterNL":null,"Description":null,"Unit":null,"Methodology":null,"Protocol":null,"QualityControl":null,"Precision":null,"Detectionlimit":null,"Instrument":null,"InstrumentUrl":null,"MethFull":"","ParaCategoryTitle":null,"ParaCatCode":null}],"dasthemes":[{"DasThemeID":26,"DasTheme":"Biology > Benthos","DasTheme0":"Benthos","DutchTerm":"Benthos"}],"projects":[{"ProID":4868,"Acronym":"ASSEMBLE+","Progress":"Completed","StandardTitle":"Association of European Marine Biological Laboratories Expanded","FP7Code":null,"GrantDOI":null,"FunderID":"730984","FunderIDType":"EU contract id","FunderCodes":["Horizon Europe"]}],"refs":null,"urls":null,"pictures":null,"urlmaps":null,"spatreps":null,"fileformats":null,"resmessage":"","complete":1}