{"datasetrec":{"DasID":8601,"Acronym":null,"StandardTitle":"Nanopore based eDNA metabarcoding of marine vertebrates in the North Sea","OrigTitle":null,"OrigTitleLangID":null,"OrigTitleLangCode":null,"OrigTitleLang":null,"OrigTitleLangNL":null,"VersionName":null,"ContactEmail":null,"VersionDate":null,"VersionDay":null,"VersionMonth":null,"VersionYear":null,"SizeReference":null,"EngAbstract":"This data is the result of DNA-based water sampling of eDNA in different locations in the North Sea.","EngDescr":"Molecular Data generation of marine biodiversity data plays a central role in monitoring and better understanding of North Sea marine ecosystems. By applying DNA-based techniques, this study specifically aims to present a metabarcoding pipeline for Nanopore sequencing and compare a newly designed 2kb primer with commonly used MiFish pimer. eDNA samples were taken in The ray reef aquarium in Dolfinarium , The brokum reef ground in the North sea, at sany bottom, Gmini windpark and around 3 shipwrecks in the North Sea and analysed through metabarcoding with nanopore sequencing and results were compared with morphology and commonly used MiFish primer in order to demonstrate the value of applying this technique.","OrigAbstract":null,"OrigDescr":null,"Comments":null,"ReleaseDate":null,"ReleaseDate0":null,"OrigDescrLang":null,"EmbargoDate":null,"OrigDescrLangNL":null,"OrigLangCode":null,"OrigLangCodeExtended":null,"OrigLangID":null,"DescrCompFlag":0,"DescrTransFlag":0,"Citation":"Doorenspleet, K.; Jansen, L.; Oosterbroek, S.; Kamermans, P.; Wurz, E.;  Murk, A.; Nijland, R.; Marine Animal Ecology; Wageningen Marine Research; Wageningen University and Research Centre: The Netherlands; (2024): Nanopore based eDNA metabarcoding of marine vertebrates in the North Sea.","AccessConstraints":null,"UDate":"2024-06-27","CDate":"2024-06-17","CurrencyDate":null,"RevisionDate":null,"DateLastModified":{"date":"2025-07-02 08:33:20.512335","timezone_type":1,"timezone":"+02:00"},"CheckedFlag":0,"PublicFlag":1,"VlizCoreFlag":1,"MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"StatusID":1,"DasType":"Data","DasTypeID":1,"DasOrigin":"Research: field experiment","Progress":"Completed","AccessConstraint":"Attribution (CC BY)","AccConstrEN":"Attribution (CC BY)","AccConstrDisplay":"<a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\"><img alt=\"Creative Commons License\" style=\"border:0px;height:15px;width:80px;vertical-align:middle;\" src=\"https://www.marinespecies.org/aphia/images/cc/by.png\" /></a> This dataset is licensed under a <a rel=\"license\" href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution 4.0 International License</a>.","License":"https://creativecommons.org/licenses/by/4.0/","AccConstrDescription":"This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.","Lineage":null,"AccConID":21},"dois":null,"spcols":null,"keywords":[{"ThesaurusTerm":"ANE, North Sea","ThesTypID":34,"ThesType":"ASFA Geoterms","Code":null,"Description":null,"OrigThesTerm":"ANE, North Sea","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Elasmobranchs","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Elasmobranchs","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Environmental DNA (eDNA)","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Environmental DNA (eDNA)","DutchTerm":"","URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Fish","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Fish","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"Metabarcoding","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"Metabarcoding","DutchTerm":null,"URI":null,"DasKeywordDescr":null},{"ThesaurusTerm":"MinION","ThesTypID":0,"ThesType":null,"Code":null,"Description":null,"OrigThesTerm":"MinION","DutchTerm":null,"URI":null,"DasKeywordDescr":null}],"parents":null,"children":null,"othrel":null,"othrelrev":null,"ownerships":[{"OrderNr":null,"Surname":"Doorenspleet","Firstname":"Karlijn","Initials":"K.","PerPublicFlag":1,"AdrID":168632,"Email":"karlijn.doorenspleet@wur.nl","InsPublicFlag":1,"Acronym":null,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"InsOwnerCNT":7,"PersID":38999,"InsID":13740,"FullInstitute":"Wageningen University and Research Centre; 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