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Microscope enumeration of coccolithophores and coccoliths was performed by filtering a 100 - 500 millilitre water sample through a Millipore HA filter, which was then rinsed with borate buffer, and frozen in a petri dish until counted. The filter was placed on a glass microscope slide, and a 60 degrees Celsius Canada Balsam was placed on top of the filter, followed by a cover slip. The clarified filter was examined for birefringent and plated coccolithophore counts, with an Olympus BH2 microscope equipped with polarisation optics. For statistical reasons, 200 coccoliths or cells were counted from each sample, when available. 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