{"projectrec":{"ProID":2103,"StandardTitle":"Microbial diversity and metal fluxes in contaminated North Sea sediments","OrigTitle":"Microbiële diversiteit en metaalfluxen in gecontamineerde Noordzeesedimenten","Acronym":"MICROMET","AbstractEnglish":"<b>Context</b>\r\n\r\nAs many heavy metals are complexed by organic matter in contaminated marine sediments, metals might be released in the water column when organic matter is degraded by microorganisms. This might affect microbial biodiversity and lead to metal bioaccumulation in higher trophic levels. To date, microbial communities in marine sediments of the Belgian Continental Plate (BCP) remain poorly studied, particularly in heavy metal contaminated zones. The MICROMET project fits within two priority research domains of the Science for a Sustainable Development programme (Biodiversity and Marine Ecosystems) and is directly connected with the aims of the Water Framework Directive (2000/60/EC).\r\n\r\n\r\n<b>Project description</b>\r\n\r\n<i>Objectives</i>\r\n\r\nThe aim of the MICROMET project is to understand the link between microbial activity and heavy metal fluxes in marine sediments. To this end, the whole microbial communities will be studied in the BCP area using an interdisciplinary approach in which geochemical and microbiological methods will be closely integrated. The research is subdivided in three Work Packages (WP 1-3). The aim of WP 1 is to determine the impact of metallic contaminants on the microbial diversity in sediments. The aim of WP 2 is to assess the importance of microorganisms in the leaching of metal contaminants from the sediments into the water column. The aim of WP 3 will be to run numerical models with the data collected during the project. This will allow predictions and lead to a better understanding of the benthic ecosystems.\r\n\r\n\r\n<i>Methodology</i>\r\n\r\n<u>WP 1.</u> \r\nAbout 10 sampling stations on the BCP will be examined during the first year. Sediments will be sampled before and after the major phytoplankton blooms. Microbial diversity will be determined using the DGGE approach. Pure cultures of microorganisms will be characterized, microbial biomass and geochemical properties of the sediment will be determined. Pore-water heavy metal concentrations will be determined using the Diffusive Equilibrium Technique (DET) and the Diffusive Gradient Technique (DGT). In the second year, sediments of 4 selected stations will be sampled monthly to take different organic matter sedimentation patterns into account. In addition to DGGE, the complete SSU rRNA will be sequenced in order to have a better understanding of the microbial diversity and physiologies.\r\n\r\n<u>WP 2.</u>\r\nVertical profiles of sediment cores from the 4 selected stations will be examined in more detail using the FISH and real-time PCR approaches, microbial productivity measurements, and detailed geochemical analyses. The central part of WP 2 will consist of laboratory simulations in which sediment cores will be incubated under various environmental conditions.\r\n\r\n<u>WP 3.</u>\r\nNumerical models will be run and validated with the data generated in this study. This will allow to predict the occurence of benthic metal fluxes taking various factors into account.\r\n\r\n\r\n<b>Interaction between the different partners</b>\r\n\r\nTwo microbiologist teams (ULB & UGent) and two geochemist teams (VUB & USTL) will work on the same sediment cores during the whole project. As a consequence, the different partners will closely interact and the microbiological data will be easily correlated with the geochemical data.\r\n\r\n\r\n<b>Expected results</b>\r\n\r\nThe MICROMET project aims at gathering new data on environmental variables and microbiological processes not previously investigated in the BCP area.\r\n\r\nSediment microbiological variables will include indices of biodiversity, SSU rRNA sequences and biomasses. Enzymatic activities and sulfate reduction rates will also be determined. These data are totally missing on the BCP.\r\n\r\nSediment geochemical variables will include oxygen, pH, S(-II), and heavy metal profiles (micro-electrodes). High-resolution heavy metal profiles will be obtained by the DET and DGT approaches. Other variables (organic C & N, etc.) will also be determined.","AbstractOtherLang":"<b>Context</b>\r\n\r\nIn vervuilde zeebodems worden zware metalen gecomplexeerd door organisch materiaal. Deze metalen kunnen vrijkomen in de waterkolom wanneer het organisch materiaal wordt afgebroken door micro-organismen. Dit kan de microbiële biodiversiteit beïnvloeden en leiden tot bioaccumulatie van metalen in hogere trofische niveaus. Tot op heden is er weinig gekend over de microbiële gemeenschappen van mariene sedimenten van de Belgische Continentale Plat (BCP), vooral in zones die vervuild zijn door zware metalen. Het MICROMET project past binnen twee prioritaire onderzoeksdomeinen van het Wetenschap voor een Duurzame Ontwikkeling Programma (Biodiversiteit en Mariene Ecosystemen) en sluit direct aan bij de doelstellingen van de Kaderrichtlijn Water (2000/60/EC).\r\n\r\n\r\n<b>Project beschrijving</b>\r\n\r\n<i>Doelstellingen</i>\r\n\r\nDe centrale doelstelling van het MICROMET project is het nastreven van een beter begrip van de link tussen de microbiële activiteit en fluxen van zware metalen in mariene sedimenten. De microbiële gemeenschappen van het BCP zullen bestudeerd worden aan de hand van een interdisciplinaire benadering waarin geochemische en microbiologische methoden nauw geïntegreerd zijn. Het onderzoek is opgedeeld in 3 werkpakketten (WP 1-3). In WP 1 zal de impact van metaalcontaminanten op de microbiële diversiteit in sedimenten nagegaan worden. De doelstelling van WP 2 is het nagaan van de rol van micro-organismen in het vrijkomen van metaalcontaminanten vanuit de sedimenten naar de waterkolom. WP 3 omvat het ontwikkelen en toepassen van numerische modellen voor de data die gedurende het project verzameld werden. Dit alles zal leiden tot een beter begrip van de benthische ecosystemen.\r\n\r\n\r\n<i>Methodologie</i>\r\n\r\n<u>WP 1.</u> \r\nGedurende het eerste jaar zullen ongeveer 10 stations van het BCP onderzocht worden. Sedimenten zullen bemonsterd worden voor en na fytoplanktonbloeien. De microbiële diversiteit zal bepaald worden aan de hand van DGGE. Reinculturen van micro-organismen zullen gekarakteriseerd worden; de microbiële biomassa en de geochemische eigenschappen van de sedimenten zullen bepaald worden. Zware metaal concentraties in het poriënwater zullen bepaald worden aan de hand van de Diffusive Equilibrium Technique (DET) en de Diffusive Gradient Technique (DGT). Gedurende het tweede jaar zullen de sedimenten van 4 geselecteerde stations maandelijks bemonsterd worden om verschillende sedimentatiepatronen in organische stof in rekening te brengen. Naast DGGE zal het volledige SSU rRNA van klonenbibliotheken gesequeneerd worden om een beter beeld te krijgen van de microbiële diversiteit en fysiologie.\r\n\r\n<u>WP 2.</u> \r\nVerticale profielen van boorkernen van 4 geselecteerde stations zullen in detail geanalyseerd worden aan de hand van FISH en real-time PCR, metingen van microbiële productiviteit, en gedetailleerde geochemische analyses. Laboratoriumsimulaties waarbij boorkernen geïncubeerd zullen worden onder verschillende milieucondities zullen centraal staan in WP 2.\r\n\r\n<u>WP 3.</u> \r\nNumerische modellen zullen ontwikkeld en gevalideerd worden aan de hand van data gegenereerd in dit project. Dit zal ons toelaten om de benthische metaalfluxen onder verschillende condities te voorspellen.\r\n\r\n\r\n<b>Interacties tussen de verschillende partners</b>\r\n\r\nTwee teams microbiologen (ULB & UGent) en twee geochemische teams (VUB & USTL) zullen op dezelfde boorkernen werken gedurende het hele project. De verschillende partners zullen aldus nauw samenwerken wat een nauwe correlatie tussen de microbiologische en geochemische data zal toelaten.\r\n\r\n\r\n<b>Verwachte resultaten</b>\r\n\r\nHet MICROMET project beoogt nieuwe data te verzamelen over microbiologische processen en milieuvariabelen die nog niet eerder bestudeerd werden op het BCP.\r\n\r\nMicrobiologische variabelen omvatten verschillende biodiversiteitsindices, SSU rRNA sequences en biomassa’s. Enzymatische activiteiten en sulfaat reductiesnelheden zullen eveneens bepaald worden. Deze data ontbraken tot op heden voor het BCP.\r\n\r\nSediment geochemische variabelen omvatten zuurstof, pH, S(-II), en zware metaal profielen (micro-electroden). Hoge-resolutie zware metaal profielen zullen bepaald worden aan de hand van DET en DGT. Andere variabelen (organische C & N, etc.) zullen eveneens bepaald worden.","DateLastModified":{"date":"2024-05-06 10:25:46.257000","timezone_type":1,"timezone":"+00:00"},"ParentProID":2063,"BeginYear":2006,"EndYear":2011,"BMonth":12,"EMonth":1,"BeginMonth":"December","EndMonth":"January","OrigTitleLangCode":"nl","OrigTitleLangID":41,"OrigTitleLangNL":"Nederlands","OrigTitleLang":"Dutch","OtherAbstractLangCode":"nl","OtherAbstractLangID":41,"OtherAbstractLang":"Dutch","OtherAbstractLangNL":"Nederlands","Progress":"Completed","ProgressNL":"Afgelopen","PublicFlag":1,"CheckedFlag":0,"ND":"2007-07-05","UD":"2011-04-14","DMPFlag":0,"Budget":883681,"BudgetCurrency":"EUR"},"parent":{"ProID":2063,"Acronym":"SSD","StandardTitle":"Science for a Sustainable Development"},"persons":null,"projects":null,"events":null,"datasets":null,"institutes":[{"instituterec":{"Acronym":"BIOMAR","ProPartID":8291,"PublicFlag":1,"OrigNameLangCode":"en","OrigNameLangID":15,"FullOrigName":"Université Libre de Bruxelles; Faculty of Sciences; Département de Biologie des Organismes; Interuniversity Center for Marine Biology (ULB - UMH); Research group Marine Biology","Line1":"Campus du Solbosch - CP160/15","Line2":"Avenue F.D. 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