{"projectrec":{"ProID":4731,"StandardTitle":"DMSP as a Reactive Oxygen Species scavenger for phytoplankton cel","OrigTitle":null,"Acronym":null,"AbstractEnglish":"The goal of this thesis is to improve knowledge about the role of dimethylsulphonopropionate (DMSP) and dimethyl sulfoxide (DMSO) as antioxidant for phytoplankton and the impact of light intensity on their production and degradation by phytoplankton. In addition to their antioxidant role for the phytoplankton, DMS(P,O) are the precursors of the dimethylsulfide (DMS), a gas affecting the climate through the production of atmospheric aerosols. The production of DMS(P,O) by phytoplankton is specific and varies with environmental conditions in particular light. The research methodology will combine laboratory experiments, field measurement and mathematical modelling. DMS(P,O) production processes and related enzymatic pathways will be studied in laboratory experiments based on monospecific cultures of key phytoplankton species in different light conditions. Proces s level studies will carried out using two complementary techniques; (1) the expression of several candidate genes implied in DMSP production will be examined using molecular techniques in parallel to (2) the production/degradation of DMSP and DMSO measured by gas chromatography for different key phytoplankton species of the Southern North Sea (SNS) in different environmental conditions of light. Reactive oxygen species (ROS; OH-, O2-, H2O2) relative production will also be measured to estimate their variability in different light conditions. Field measurements will allow to quantify the importance of DMS(P,O) production and DMS emission in the SNS area related to phytoplankton diversity and environmental factors. Data from laboratory experiments and field measurements will then be used to calibrate and validate the DMS(P,O) dynamics in the biogeochemical MIRO-DMS model applied to the SNS. Model simulations will allow to (i) determine the relative importance of biotic and abiotic fa ctors in the production of DMS(P,O) by aquatic ecosystems and (ii) improve the prediction of current and future emissions of DMS.","AbstractOtherLang":"Het doel van dit proefschrift is om de kennis over de rol van dimethylsulphonopropionate (DMSP) en dimethylsulfoxide (DMSO) als antioxidant voor fytoplankton en de impact van de lichtintensiteit over hun productie en afbraak door fytoplankton te verbeteren. Naast hun antioxidant rol van fytoplankton, DMS (P, O) zijn de voorlopers van dimethylsulfide (DMS), een gas die de klimaat door de productie van atmosferische aërosolen. De productie van DMS (P, O) door fytoplankton is specifiek en varieert met de milieuomstandigheden in het bijzonder licht. De onderzoeksmethode zal laboratoriumexperimenten, veldmetingen en wiskundige modellen te combineren. DMS (P, O) productieprocessen en aanverwante enzymatische routes zullen worden onderzocht in het laboratorium experimenten gebaseerd op monospecifieke culturen van de belangrijkste soorten fytoplankton in verschillend e lichtomstandigheden. Procesniveau studies zullen worden uitgevoerd met behulp van twee complementaire technieken; (1) de expressie van verschillende kandidaat-genen geïmpliceerd in DMSP productie zal worden onderzocht met behulp van moleculaire technieken parallel aan (2) de productie / afbraak van DMSP en DMSO gemeten met gaschromatografie andere sleutel fytoplankton van de Zuidelijke Noordzee (SNS) in verschillende milieu-omstandigheden van het licht. Reactive oxygen species (ROS, OH, O2-, H2O2) ten opzichte van de productie zal ook worden gemeten om hun variabiliteit te schatten in verschillende lichtomstandigheden. Veldmetingen zal toelaten om het belang van DMS (P, O) productie en DMS-emissie te kwantificeren in het SNS gebied met betrekking tot diversiteit en omgevingsfactoren fytoplankton. Gegevens van laboratoriumexperimenten en veldmetingen wordt vervolgens gebruikt voor kalibreren en valideren van de DMS (P, O) dynamiek in de biochemische MIRO-DMS model toegepast op de SNS. Model simulaties zal toelaten om (i) het bepalen van de relatieve belang van biotische en abiotische factoren in de productie van DMS (P, O) van aquatische ecosystemen en (ii) het verbeteren van de voorspelling van de huidige en toekomstige emissies van DMS.","DateLastModified":{"date":"2024-05-06 10:25:46.257000","timezone_type":1,"timezone":"+00:00"},"ParentProID":null,"BeginYear":null,"EndYear":null,"BMonth":null,"EMonth":null,"BeginMonth":null,"EndMonth":null,"OrigTitleLangCode":null,"OrigTitleLangID":null,"OrigTitleLangNL":null,"OrigTitleLang":null,"OtherAbstractLangCode":"nl","OtherAbstractLangID":41,"OtherAbstractLang":"Dutch","OtherAbstractLangNL":"Nederlands","Progress":null,"ProgressNL":null,"PublicFlag":1,"CheckedFlag":0,"ND":"2017-04-11","UD":"2017-04-11","DMPFlag":0,"Budget":null,"BudgetCurrency":"EUR"},"parent":null,"persons":null,"projects":null,"events":null,"datasets":null,"institutes":[{"instituterec":{"Acronym":"ULB","ProPartID":16140,"PublicFlag":1,"OrigNameLangCode":null,"OrigNameLangID":null,"FullOrigName":null,"Line1":"Franklin Rooseveltlaan 50","Line2":"1050 Brussel","Line3":null,"Line4":null,"InsID":4535,"FullStandardName":"Université Libre de Bruxelles","Role":"Partner","RoleID":17,"EncAddress":"Franklin Rooseveltlaan 50, 1050 Brussel, Belgium"},"parent":null,"institutes":null,"references":null,"conferences":null,"datasets":null,"persons":[{"Surname":"Royer","Firstname":"Colin","Initials":"C.","LeaderFlag":0,"PersID":34291,"Role":null,"RoleID":null,"PastInstitute":null,"BeginDay":null,"BeginMonth":null,"BeginYear":null,"EndDay":null,"EndMonth":null,"EndYear":null}],"pastpers":null,"subpers":null,"projects":null,"urls":null,"pictures":null,"published":null,"affrefs":null,"collections":null,"thesterms":null,"taxterms":null,"geoterms":null,"thestermsFRIS":null,"nXtins":null,"previns":null,"spcols":null,"resmessage":"no id specified","complete":0,"participantrec":null,"peerrevs":null,"urlmaps":null}],"refs":null,"urls":null,"thesterms":null,"taxterms":null,"geoterms":null,"funderids":null,"othtermsFRIS":null,"pictures":[],"spcols":null,"resmessage":"","complete":1}