{"projectrec":{"ProID":939,"StandardTitle":"The effect of UV-B radiation on the nitrogen metabolism of phytoplancton","OrigTitle":"De invloed van UV-B straling op het stikstofmetabolisme van fytoplankton","Acronym":null,"AbstractEnglish":"Recent research concerns aim at a better understanding of the damaging effect of enhanced UV- B radiation on growth and productivity of plants. Unicellular algae as well as higher plants exhibit malfunction under UV-B stress. Considerable changes in nitrogen metabolism were observed but are still poorly understood. Generally it is thought that the uptake of inorganic nitrogenous nutrients is hindered bu increased UV-B radiation and that severe reduction of the amino acid and protein sunthesis occurs. This study will assess the effect of UV-B on the nitrogen uptake regime and on the flow of nitrogen in the metabolic pathhways of selected phytoplankton species, known to have an important role in aquatic ecosystems. Therefore, a study of the nitrate and ammonium uptake and assimilation will be undertaken. The determination of absolute and specific uptake rates relies on the use of the 15N-methodology. The amino acid and protein synthesis in phytoplancton will be studied by means of analytical and preparative chromatography in combination with 15N tracer studies. Special attention will go to the specific target sites of UV-B radiation. The planned research is a collaboration between the department of ANCH and ORGC.","AbstractOtherLang":"Recente onderzoekingen trachtten de storende invloed van verhoogde UV-B straling op de groei en productiviteit van planten beter in te schatten. Het is bekend dat zowel eencelligen als hogere planten afijkingen vertonen onder UV-B stress. Er werden reeds wijzigingen in het stikstofmetabolisme waargenomen, maar die zijn nog onvoldoende verklaard. De opname van anorganische nutriënten verloopt trager en zowel aminozuur- als proteosynthese  zijn gereduceerd. Deze studie wil de invloed van UV-B op de opname van anorganisch stiktof en op de metabolische stofstromen voor enkele welbepaalde futoplanktonsoorten bestuderen. Voor de bepaling van de specifieke en absolute opnamesnelheden van nitraat en ammonium zal gebruik  gemaakt worden van de 15N-methodologie. De studie van aminozuur- en proteosynthese berust op het gebruik van analytische en preparatieve chromatografie in combinatie met de 15N-methodologie. Speciale aandacht gaat naar de specifieke UV-B gevoelige sites in de sdtikstofkringloop. Het onderzoek is een samenwerking tussen de afdelingen ANCH en ORGC.","DateLastModified":{"date":"2025-07-02 08:33:20.809226","timezone_type":1,"timezone":"+02:00"},"ParentProID":null,"BeginYear":1994,"EndYear":null,"BMonth":null,"EMonth":null,"BeginMonth":null,"EndMonth":null,"OrigTitleLangCode":"nl","OrigTitleLangID":41,"OrigTitleLangNL":"Nederlands","OrigTitleLang":"Dutch","OtherAbstractLangCode":"nl","OtherAbstractLangID":41,"OtherAbstractLang":"Dutch","OtherAbstractLangNL":"Nederlands","Progress":null,"ProgressNL":null,"PublicFlag":1,"CheckedFlag":0,"ND":"2003-11-26","UD":"2007-04-02","DMPFlag":0,"Budget":null,"BudgetCurrency":null},"parent":null,"persons":null,"projects":null,"events":null,"datasets":null,"institutes":[{"instituterec":{"Acronym":"AMGC","ProPartID":1017,"PublicFlag":1,"OrigNameLangCode":"en","OrigNameLangID":15,"FullOrigName":"Vrije Universiteit Brussel; Research group Analytical, Environmental and Geochemistry","Line1":"Pleinlaan 2","Line2":"1050 Brussel","Line3":null,"Line4":null,"InsID":40,"FullStandardName":"Vrije Universiteit Brussel; Faculteit Wetenschappen & Bio-ingenieurswetenschappen; Vakgroep Chemie; Analytical, Environmental and Geochemistry","Role":"Co-ordinator","RoleID":7,"EncAddress":"Pleinlaan 2, 1050 Brussel, Belgium"},"parent":null,"institutes":null,"references":null,"conferences":null,"datasets":null,"persons":[{"Surname":"Goeyens","Firstname":"Leo","Initials":"L.","LeaderFlag":0,"PersID":154,"Role":null,"RoleID":null,"PastInstitute":1,"BeginDay":null,"BeginMonth":null,"BeginYear":null,"EndDay":null,"EndMonth":null,"EndYear":null}],"pastpers":null,"subpers":null,"projects":null,"urls":null,"pictures":null,"published":null,"affrefs":null,"collections":null,"thesterms":null,"taxterms":null,"geoterms":null,"thestermsFRIS":null,"nXtins":null,"previns":null,"spcols":null,"resmessage":"no id specified","complete":0,"participantrec":null,"peerrevs":null,"urlmaps":null}],"refs":null,"urls":null,"thesterms":[{"ThesaurusTerm":"Belgium","ThestID":183110,"ThesTypID":34,"ThesType":"ASFA Geoterms","OrigThesTerm":"Belgium","DutchTerm":null,"Code":null},{"ThesaurusTerm":"Nitrogen","ThestID":5565,"ThesTypID":1,"ThesType":"ASFA Thesaurus List","OrigThesTerm":"Nitrogen","DutchTerm":"Stikstof","Code":"c_5192"},{"ThesaurusTerm":"Nutrients (mineral)","ThestID":5629,"ThesTypID":1,"ThesType":"ASFA Thesaurus List","OrigThesTerm":"Nutrients (mineral)","DutchTerm":"Nutriënten","Code":null},{"ThesaurusTerm":"Phytoplankton","ThestID":6208,"ThesTypID":1,"ThesType":"ASFA Thesaurus List","OrigThesTerm":"Phytoplankton","DutchTerm":"Fytoplankton","Code":"c_13344"},{"ThesaurusTerm":"Plankton","ThestID":6265,"ThesTypID":1,"ThesType":"ASFA Thesaurus List","OrigThesTerm":"Plankton","DutchTerm":"Plankton","Code":"c_5950"},{"ThesaurusTerm":"Ultraviolet radiation","ThestID":8836,"ThesTypID":1,"ThesType":"ASFA Thesaurus List","OrigThesTerm":"Ultraviolet radiation","DutchTerm":null,"Code":"c_8046"}],"taxterms":null,"geoterms":[{"GeoTerm":"Belgium","GeoObjectID":14,"OrigGeoTerm":"Belgium","DutchTerm":"België"}],"funderids":[{"FunderID":"P101/2942210491","ThestID":181476,"FunderType":"Other contract id"}],"othtermsFRIS":null,"pictures":[],"spcols":null,"resmessage":"","complete":1}