{"refrec":{"BRefID":100061,"RR":"<b>Viant, M.R.; Pincetich, C.A.; Tjeerdema, R.S.</b> (2006). Metabolic effects of dinoseb, diazinon and esfenvalerate in eyed eggs and alevins of Chinook salmon (<i>Oncorhynchus tshawytscha</i>) determined by 1H NMR metabolomics. <i>Aquat. Toxicol. 77(4)</i>: 359-371. <a href=\"http://dx.doi.org/10.1016/j.aquatox.2006.01.009\" target=\"_blank\">http://dx.doi.org/10.1016/j.aquatox.2006.01.009</a>","BEntID":95313,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 77(4)</i>: 359-371. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2006.01.009\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2006.01.009</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Viant, M.R.; Pincetich, C.A.; Tjeerdema, R.S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Viant, M.R. <i>et al.</i>","Englishabstract":"Pesticide pulses in the Sacramento River, California, originate from storm-water discharges and non-point source aquatic pollution that can last from a few days to weeks. The Sacramento River and its tributaries have historically supported the majority of California's Chinook salmon (<i>Oncorhynchus tshawytscha</i>) spawning grounds. Three pesticides currently used in the Sacramento Valley &#x2013; dinoseb, diazinon, and esfenvalerate &#x2013; were chosen to model the exposure of salmon embryos to storm-water discharges. Static-renewal (96&nbsp;h) exposures to eyed eggs and alevins resulted in both toxicity and significant changes in metabolism assessed in whole-embryo extracts by <sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy based metabolomics and HPLC with UV detection (HPLC-UV). The 96-h LC<sub>50</sub> values of eyed eggs and alevins exposed to dinoseb were 335 and 70.6&nbsp;ppb, respectively, and the corresponding values for diazinon were 545 and 29.5&nbsp;ppm for eyed eggs and alevins, respectively. The 96-h LC<sub>50</sub> of eyed eggs exposed to esfenvalerate could not be determined due to lack of mortality at the highest exposure concentration, but in alevins was 16.7&nbsp;ppb. All esfenvalerate exposed alevins developed some degree of lordosis or myoskeletal abnormality and did not respond to stimulus or exhibit normal swimming behavior. ATP concentrations measured by HPLC-UV decreased significantly in eyed eggs due to 250&nbsp;ppb dinoseb and 10 and 100&nbsp;ppb esfenvalerate (<i>p</i>&nbsp;&#60;&nbsp;0.05). Phosphocreatine, as measured by HPLC-UV, decreased significantly in eyed eggs due to 250&nbsp;ppb dinoseb, 10 and 100&nbsp;ppb esfenvalerate, and 100&nbsp;ppm diazinon (<i>p</i>&nbsp;&#60;&nbsp;0.05). Principal components analyses of <sup>1</sup>H NMR metabolite fingerprints of eyed egg and alevin extracts revealed both dose-dependent and mechanism of action-specific metabolic effects induced by the pesticides. Furthermore, NMR based metabolomics proved to be more sensitive than HPLC-UV in identifying significant changes in sublethal metabolism of pesticide exposed alevins. In conclusion, we have demonstrated several benefits of a metabolomics approach for chemical risk assessment, when used in conjunction with a fish embryo assay, and have identified significant metabolic perturbations to the early life stages of Chinook salmon by currently used pesticides.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Metabolic effects of dinoseb, diazinon and esfenvalerate in eyed eggs and alevins of Chinook salmon (<i>Oncorhynchus tshawytscha</i>) determined by 1H NMR metabolomics","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"salmon; embryo; toxicity; diazinon; dinoseb; esfenvalerate; NMR;metabolomics","OtherDescriptors":null,"Notes":null,"AnaPub":2006,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2006-06-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000237813400003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2006.01.009"},"refs":null,"anarec":{"AnaID":100061,"PubliDate":2006,"Pagination":"359-371","XtraPublOfAnaID":null,"ISBN":null,"Volume":"77","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":359,"Pages":13,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Viant","Firstname":"Mark","Initials":"M.R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":95313,"AutID":35821,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Pincetich","Firstname":"Christopher","Initials":"C.A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":95313,"AutID":66991,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Tjeerdema","Firstname":null,"Initials":"R.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":95313,"AutID":31164,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier Science","City":"Tokyo; New York; London; Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Embryos","ThestID":2747,"Acronym":"ASFA","ThesTermPath":"Developmental stages > Embryos"},{"ThesaurusTerm":"Toxicity","ThestID":8666,"Acronym":"ASFA","ThesTermPath":"Properties > Biological properties > Toxicity"}],"thestermsASFA":[{"ThesaurusTerm":"Embryos"},{"ThesaurusTerm":"Toxicity"}],"taxtermsASFA":[{"TaxTerm":"Oncorhynchus tshawytscha"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":95313,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":95313,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.aquatox.2006.01.009","externalID":"10.1016/j.aquatox.2006.01.009","URLTypeCode":"DOI","URLID":5256,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Embryos","ThestID":2747,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Toxicity","ThestID":8666,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Oncorhynchus tshawytscha","AphiaID":158075,"TaxtID":23128}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Embryos","DutchTerm":null,"ThestID":2747,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Toxicity","DutchTerm":"Toxiciteit","ThestID":8666,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Oncorhynchus tshawytscha","DutchTerm":null,"AphiaID":158075,"TaxtID":23128}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2006-06-22 09:24:04.390000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lyssens, Liesbeth, L.","updSesDate":{"date":"2018-05-17 21:28:56.790000","timezone_type":3,"timezone":"Europe/Brussels"}}}