{"refrec":{"BRefID":100221,"RR":"<b>Jonsson, H.; Schiedek, D.; Grøsvik, B.E.; Goksøyr, A.</b> (2006). Protein responses in blue mussels (<i>Mytilus edulis</i>) exposed to organic pollutants: a combined CYP-antibody/proteomic approach. <i>Aquat. Toxicol. 78(Supplement 1)</i>: S49-S56. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2006.02.024\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2006.02.024</a>","BEntID":329382,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Toxicol. 78(Supplement 1)</i>: S49-S56. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2006.02.024\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2006.02.024</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Jonsson, H.; Schiedek, D.; Grøsvik, B.E.; Goksøyr, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Jonsson, H. <i>et al.</i>","Englishabstract":"Polyclonal antibodies were raised against highly conserved, trans-metazoan sequences of cytochrome P450 (CYP) families 2 and 4 and used to investigate responses in the common blue mussel (<i>Mytilus edulis</i>) exposed to various organic contaminants. The results were evaluated by means of cross-reacting proteins on Western blots of both one- and two-dimensional electrophoresis gels, and by scanning spectroscopy measurements of total CYP content. Furthermore, a proteomic approach was applied aimed at elucidating exposure-related protein changes in a more general term. Identities of isolated proteins were searched by means of peptide mass fingerprints obtained from MALDI-TOF MS analyses.<p>The results demonstrated that both antibodies rendered several cross-reactive bands when probed on Western blots. The most obvious cross-reaction of the CYP2 antibody was with a strongly expressed protein of size ≈57 kDa, p<i>I</i> 4.5–4.6, whereas the CYP4 antibody cross-reacted with a protein of size ≈55 kDa, p<i>I</i> 5.6. However, expression of cross-reacting proteins did not change as a result of the exposures, and resulted only in small and insignificant fluctuations in total CYP content. As a contrast, silver-stained 2DE gels showed that several microsomal proteins were affected in individuals exposed to diallylphthalate as well as crude oil, with and without a spike of alkylphenols and PAHs. Mass spectrometry based analyses of excised, trypsin-digested spots did so far not decipher the identities of the proteins affected by the exposures, nor of those cross-reacting with CYP2 and CYP4 antibodies.<p>This study has underlined the power of the proteomic approach in environmental toxicology, although protein identification was not successful. The missing identities of the proteins cross-reacting with the CYP2- and CYP4-antibodies does not enable a clear conclusion as to whether or not these peptides actually represent CYP iso-enzymes.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Protein responses in blue mussels (<i>Mytilus edulis</i>) exposed to organic pollutants: a combined CYP-antibody/proteomic approach","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-17 01:31:38.020031","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"cytochrome P450; CYP2; CYP4; mussel; oil; phthalates; proteomics;two-dimensional electrophoresis","OtherDescriptors":null,"Notes":null,"AnaPub":2006,"MonPub":null,"DateUpdate":"2021-02-19","DateCreate":"2006-06-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000238130600007","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2006.02.024"},"refs":null,"anarec":{"AnaID":100221,"PubliDate":2006,"Pagination":"S49-S56","XtraPublOfAnaID":null,"ISBN":null,"Volume":"78","Issue":"Supplement 1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":[{"YBRefID":100196,"RefStrFull":"<b>Pampanin, D.M.; Anderson, O.K.; Viarengo, A. (Ed.)</b> (2006). The Stavanger Workshop: Biological Effects of Environmental Pollution (BEEP) in marine coastal ecosystem: the Stavanger mesocosm exposure studies. <i>Aquatic Toxicology</i>, Special Issue 78(Suppl. 1). Elsevier: The Netherlands. S1-S128 pp.","RelID":10,"Relation":"Also appears in","StandardTitle":"The Stavanger Workshop: Biological Effects of Environmental Pollution (BEEP) in marine coastal ecosystem: the Stavanger mesocosm exposure studies"}],"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Jonsson","Firstname":"Henrik","Initials":"H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":329382,"AutID":67064,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Schiedek","Firstname":"Doris","Initials":"D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":329382,"AutID":21636,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":5302,"InsID":null},{"AutName":"Grøsvik","Firstname":"Bjørn Einar","Initials":"B.E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":329382,"AutID":67066,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Goksøyr","Firstname":"Anders","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":329382,"AutID":67065,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier Science","City":"Tokyo; 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