{"refrec":{"BRefID":101696,"RR":"<b>Deane, E.E.; Woo, N.Y.S.</b> (2006). Impact of heavy metals and organochlorines on hsp70 and hsc70 gene expression in black sea bream fibroblasts. <i>Aquat. Toxicol. 79(1)</i>: 9-15. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2006.04.009\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2006.04.009</a>","BEntID":96784,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Toxicol. 79(1)</i>: 9-15. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2006.04.009\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2006.04.009</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Deane, E.E.; Woo, N.Y.S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Deane, E.E.; Woo, N.Y.S.","Englishabstract":"The aim of the present study was to investigate the effects of environmentally important heavy metals and organochlorines on the transcriptional profiles of genes coding for heat shock cognate 70 (<i>hsc70</i>) and inducible heat shock protein 70 (<i>hsp70</i>) in a black sea bream fibroblast cell line. Using the nucleotide sequence information, from the cloned genes, specific reverse transcriptase-polymerase chain reaction (RT-PCR) methods were devised to test the effects of heavy metals (Cd<sup>2+</sup>, Cu<sup>2+</sup>and Ni<sup>2+</sup>) and organochlorines (aroclor 1254, hexachlorobenzene and 2-4-dichloroaniline) on the cell stress response. Hsp70 was induced in fibroblasts upon heavy metal exposure concentrations as low as 0.01 μM whereas <i>hsc70</i> expression was induced upon organochlorine exposure concentrations as low as 0.001 μM. Overall, our findings demonstrate that gene members of the HSP70 family are responsive to environmentally important chemicals.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Impact of heavy metals and organochlorines on hsp70 and hsc70 gene expression in black sea bream fibroblasts","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-14 01:31:13.203506","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2006,"MonPub":null,"DateUpdate":"2020-10-20","DateCreate":"2006-08-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000239675900002","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2006.04.009"},"refs":null,"anarec":{"AnaID":101696,"PubliDate":2006,"Pagination":"9-15","XtraPublOfAnaID":null,"ISBN":null,"Volume":"79","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":9,"Pages":7,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"The Chinese University of Hong Kong,","Email":"normanwoo@cuhk.edu.hk","Line2":null,"EnvName":"China"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Deane","Firstname":"Eddie","Initials":"E.E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":96784,"AutID":68003,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Woo","Firstname":"Norman","Initials":"N.Y.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":96784,"AutID":72030,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":352938,"LibID":36,"BRefID":101696,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"352938","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier Science","City":"Tokyo; New York; London; Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Marine fish","ThestID":5000,"Acronym":"ASFA","ThesTermPath":"Aquatic organisms > Marine organisms > Fish > Marine fish"},{"ThesaurusTerm":"Proteins","ThestID":6602,"Acronym":"ASFA","ThesTermPath":"Chemical compounds > Organic compounds > Proteins"},{"ThesaurusTerm":"Polymerase chain reaction","ThestID":9583,"Acronym":"ASFA","ThesTermPath":"Polymerase chain reaction"}],"thestermsASFA":[{"ThesaurusTerm":"Marine fish"},{"ThesaurusTerm":"Polymerase chain reaction"},{"ThesaurusTerm":"Proteins"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":96784,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":96784,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.aquatox.2006.04.009","externalID":"10.1016/j.aquatox.2006.04.009","URLTypeCode":"DOI","URLID":87018,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Marine fish","ThestID":5000,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Polymerase chain reaction","ThestID":9583,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Proteins","ThestID":6602,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Marine fish","DutchTerm":"Mariene vis","ThestID":5000,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Polymerase chain reaction","DutchTerm":"Polymerase kettingreactie","ThestID":9583,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Proteins","DutchTerm":"Eiwitten","ThestID":6602,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2006-08-24 09:20:00.727000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2020-10-20 08:17:57.067000","timezone_type":3,"timezone":"Europe/Brussels"}}}