Document of bibliographic reference 102590

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AM

Title

Distribution and ecotoxicity of chlorotriazines in the Scheldt estuary (Belgium-Netherlands)

Abstract

The continuous introduction of new products used as growth promoters in animal husbandry, for sports doping and as products for body-building requires residue laboratories to initiate research on developing a strategy for the identification of ’unknown’ components. In this study, a strategy is presented for elucidating the identity, the structure and the possible effects of illegal estrogenic compounds in an unidentified water-based solution. To obtain complete information on the composition and activity of the unidentified product, a multidisciplinary approach was needed. A case-study is described with a ’solution X’ found during a raid. First, in vivo techniques (animal trials with mice, anatomical and histological research) were combined with in vitro techniques (the yeast estrogenic screen (YES)). In a later stage of the investigation, HPLC-fractionation, liquid chromatography–multiple mass spectrometry (LC-MS ⁿ and gas chromatography-multiple mass spectrometry (GC-MS ⁿ were used. Finally, the identity of ’solution X’ was confirmed in a very low concentration range (10 ng/L estrone and 400 ng/l ethinyloestradiol).

Bibliographic citation

Noppe, H.; Ghekiere, A.; Verslycke, T.; De Wulf, E.; Verheyden, K.; Monteyne, E.; Polfliet, K.; Van Caeter, P.; Janssen, C.R.; De Brabander, H.F. (2006). Distribution and ecotoxicity of chlorotriazines in the Scheldt estuary (Belgium-Netherlands), in: Noppe, H. Analytiek van hormoon verstorende stoffen in milieumatrices = Analytics of endocrine disrupting chemicals in environmental matrices. pp. 85-105

Topic

Marine