{"refrec":{"BRefID":102826,"RR":"<b>McDonagh, B.; Sheehan, D.</b> (2006). Redox proteomics in the blue mussel <i>Mytilus edulis</i>: carbonylation is not a pre-requisite for ubiquitination in acute free radical-mediated oxidative stress. <i>Aquat. Toxicol. 79(4)</i>: 325-333. <a href=\"http://dx.doi.org/10.1016/j.aquatox.2006.06.020\" target=\"_blank\">http://dx.doi.org/10.1016/j.aquatox.2006.06.020</a>","BEntID":99614,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 79(4)</i>: 325-333. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2006.06.020\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2006.06.020</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"McDonagh, B.; Sheehan, D.","OrigTitleTranslFlag":0,"Authorstringtrunc":"McDonagh, B.; Sheehan, D.","Englishabstract":"Mytilus edulis</i> was exposed under controlled conditions to a panel of model pro-oxidants (H<sub>2</sub>O<sub>2</sub>, CdCl<sub>2</sub> and menadione) for 24&nbsp;h. Protein extracts of gill, mantle and digestive gland were analysed by immunoblotting in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) separations. Immunoblotting revealed extensive and comparable levels of protein carbonylation across the pro-oxidant panel with approximately 1.5-fold higher levels in gill than digestive gland. Ubiquitination in gill was modest in response to H<sub>2</sub>O<sub>2</sub>, but increased in response to menadione and CdCl<sub>2</sub>. High ubiquitination levels were found for all pro-oxidants in digestive gland with levels comparable to the highest found in gill. Two-dimensional (2D) SDS-PAGE confirmed specific targeting of individual proteins by ubiquitin against a generally stable protein expression signature. Spot matching suggested that carbonylation is not a pre-requisite for ubiquitination. While gill showed consistently higher constitutive levels of glutathione transferase, glucose 6-phosphate dehydrogenase and glutathione reductase activity, pro-oxidant treatment had only modest effect on these enzymes and on the ratio of reduced/oxidised glutathione. In digestive gland, this latter ratio was higher than in gill and increased in response to menadione and CdCl<sub>2</sub>. Ubiquitination may provide a marker for acute onset of oxidative stress in bivalves.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Redox proteomics in the blue mussel <i>Mytilus edulis</i>: carbonylation is not a pre-requisite for ubiquitination in acute free radical-mediated oxidative stress","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"carbonylation; ubiquitination; ubiquitin; glutathione; GST; glucose6-phosphate dehydrogenase; oxidative stress; 2D SDS-PAGE; proteomics;Mytilus edulis","OtherDescriptors":null,"Notes":null,"AnaPub":2006,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2006-10-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000241090300002","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2006.06.020"},"refs":null,"anarec":{"AnaID":102826,"PubliDate":2006,"Pagination":"325-333","XtraPublOfAnaID":null,"ISBN":null,"Volume":"79","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. 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