{"refrec":{"BRefID":102829,"RR":"<b>Puinean, A.M.; Labadie, P.; Hill, E.M.; Osada, M.; Kishida, M.; Nakao, R.; Novillo, A.; Callard, I.P.; Rotchell, J.M.</b> (2006). Laboratory exposure to 17ß-estradiol fails to induce vitellogenin and estrogen receptor gene expression in the marine invertebrate <i>Mytilus edulis</i>. <i>Aquat. Toxicol. 79(4)</i>: 376-383. <a href=\"http://dx.doi.org/10.1016/j.aquatox.2006.07.006\" target=\"_blank\">http://dx.doi.org/10.1016/j.aquatox.2006.07.006</a>","BEntID":99617,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 79(4)</i>: 376-383. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2006.07.006\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2006.07.006</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Puinean, A.M.; Labadie, P.; Hill, E.M.; Osada, M.; Kishida, M.; Nakao, R.; Novillo, A.; Callard, I.P.; Rotchell, J.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Puinean, A.M. <i>et al.</i>","Englishabstract":"To investigate the effect of estrogenic compounds on the marine mussel <i>Mytilus edulis</i>, an assay was developed to measure the expression of two vertebrate estrogen responsive genes&#x2014;estrogen receptor (<i>ER</i>) and vitellogenin (<i>VTG</i>) genes. Expression was measured in <i>M. edulis</i> gonads following a 10-day exposure to 200&nbsp;ng/l 17&#x3b2;-estradiol (estradiol). The concentrations of esterified estradiol in mussel tissue increased 15-fold in a time-dependent manner&#x2014;confirming uptake of the compound by the mussels, however there was no significant increase of free estradiol in mussel tissues during the exposure period. The <i>ER</i> and <i>VTG</i> mRNA levels in the gonads of both sexes were measured at days 1&#x2013;3, 5, and 10 in control and exposed mussels. However, no significant change in the expression of either the <i>ER</i> or <i>VTG</i> genes was recorded at any of the sampled time points. The results suggest that either a regulatory mechanism exists in a mussel that is able to maintain constant levels of free estradiol by converting the excess estradiol into esterified products which may have reduced affinity for the estrogen receptor, or alternatively, that the <i>ER</i> and <i>VTG</i> genes are unresponsive to estrogens in these organisms. The significance of these findings in terms of the utility of <i>ER</i> and <i>VTG</i> as biomarkers of endocrine disruption in bivalve species is discussed.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Laboratory exposure to 17ß-estradiol fails to induce vitellogenin and estrogen receptor gene expression in the marine invertebrate <i>Mytilus edulis</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"vitellogenin; mussel; endocrine disruption; biomarker","OtherDescriptors":null,"Notes":null,"AnaPub":2006,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2006-10-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000241090300007","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2006.07.006"},"refs":null,"anarec":{"AnaID":102829,"PubliDate":2006,"Pagination":"376-383","XtraPublOfAnaID":null,"ISBN":null,"Volume":"79","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. 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