{"refrec":{"BRefID":102830,"RR":"Hassenklöver, T.; Bickmeyer, U. (2006). The marine secondary metabolites 2,4-dibromophenol and 2,4,6-tribromophenol differentially modulate voltage dependent ion currents in neuroendocrine (PC12) cells. Aquat. Toxicol. 79(4): 384-390. https://dx.doi.org/10.1016/j.aquatox.2006.07.002","BEntID":99618,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". Aquat. Toxicol. 79(4): 384-390. https://dx.doi.org/10.1016/j.aquatox.2006.07.002","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hassenklöver, T.; Bickmeyer, U.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hassenklöver, T.; Bickmeyer, U.","Englishabstract":"2,4-Dibromophenol (2,4-DBP) and 2,4,6-tribromophenol (2,4,6-TBP) are marine secondary metabolites, with 2,4,6-tribromophenol playing an important role as industrially produced flame retardant and pesticide. Both substances disturb cellular calcium signals in neuroendocrine cells as previously shown by Hassenklöver et al. (2006) [Hassenklöver, T., Predehl, S., Pilli, J., Ledwolorz, J., Assmann, M., Bickmeyer, U., 2006. Bromophenols, both present in marine organisms and in industrial flame retardants, disturb cellular Ca2+ signaling in neuroendocrine cells (PC12). Aquat. Toxicol. 76, 37–45]. We investigated calcium channel currents in detail and outward membrane currents as potential cellular targets of both bromophenols. In this electrophysiological approach, 2,4-DBP reduced voltage dependent calcium channel currents with a half-maximal concentration of 45 ± 32 μM (S.D.) and a Hill coefficient of 0.87 ± 0.49 (S.D.). 2,4,6-TBP reduced calcium channel currents with a half-maximal concentration of 28 ± 19 μM (S.D.) and a Hill coefficient of 0.79 ± 0.31 (S.D.). The major contribution to calcium channel currents was mediated by L-type (67%) and N-type channels (30%) in PC12 cells; both bromophenols modulated both current types. Whole cell outward currents, mainly carried by potassium ions, were reduced by 2,4-DBP with a half-maximal concentration of 41 ± 9 μM (S.D.) showing a Hill coefficient of 1.71 ± 0.31 (S.D.). 2,4,6-TBP showed a weak reduction of outward currents at high concentrations of 300 μM. 2,4,6-TBP selectively decreased calcium entry via calcium channels as revealed in whole cell patch clamp experiments, whereas 2,4-DBP reduced both in- and outward currents.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The marine secondary metabolites 2,4-dibromophenol and 2,4,6-tribromophenol differentially modulate voltage dependent ion currents in neuroendocrine (PC12) cells","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-12 01:31:14.761057","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"bromophenol; calcium channel; neurotoxicology; flame retardant; hormones","OtherDescriptors":null,"Notes":null,"AnaPub":2006,"MonPub":null,"DateUpdate":"2020-10-21","DateCreate":"2006-10-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000241090300008","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2006.07.002"},"refs":null,"anarec":{"AnaID":102830,"PubliDate":2006,"Pagination":"384-390","XtraPublOfAnaID":null,"ISBN":null,"Volume":"79","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":384,"Pages":7,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"Alfred Wegener Institut für Polar- und","Email":"ubickmeyer@awi.de","Line2":"Meeresforschung in der Helmholtz Gemeinschaft, Kurpromenade 201, 27483 Helgoland","EnvName":"Germany"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Hassenklöver","Firstname":"Thomas","Initials":"T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":99618,"AutID":57000,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bickmeyer","Firstname":"Ulf","Initials":"U.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":99618,"AutID":57005,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":352997,"LibID":36,"BRefID":102830,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"352997","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
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