{"refrec":{"BRefID":107286,"RR":"<b>Escobedo-Bonilla, C.M.; Audoorn, L.; Wille, M.; Alday-Sanz, V.; Sorgeloos, P.; Pensaert, M.B.; Nauwynck, H.J.</b> (2006). Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes. <i>Dis. Aquat. Org. 68(3)</i>: 181-188. <a href=\"http://dx.doi.org/10.3354/dao068181\" target=\"_blank\">dx.doi.org/10.3354/dao068181</a>","BEntID":102122,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Dis. Aquat. Org. 68(3)</i>: 181-188. <a href=\"https://dx.doi.org/10.3354/dao068181\" target=\"_blank\">https://dx.doi.org/10.3354/dao068181</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Escobedo-Bonilla, C.M.; Audoorn, L.; Wille, M.; Alday-Sanz, V.; Sorgeloos, P.; Pensaert, M.B.; Nauwynck, H.J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Escobedo-Bonilla, C.M. <i>et al.</i>","Englishabstract":"In the past, strategies to control white spot syndrome virus (WSSV) were mostly tested by infectivity trials in vivo using immersion or per os inoculation of undefined WSSV infectious doses, which complicated comparisons between experiments. In this study, the reproducibility of 3 defined doses (10, 30 and 90 shrimp infectious doses 50% endpoint [SID<sub>50</sub>] of WSSV was determined in 3 experiments using intramuscular (i.m.) or oral inoculation in specific pathogen-free (SPF) <i>Litopenaeus vannamei</i>. Reproducibility was determined by the time of onset of disease, cumulative mortality, and median lethal time (LT<sub>50</sub>). By i.m. route, the 3 doses induced disease between 24 and 36 h post inoculation (hpi). Cumulative mortality was 100% at 84 hpi with doses of 30 and 90 SID<sub>50</sub> and 108 hpi with a dose of 10 SID<sub>50</sub>. The LT<sub>50</sub> of the doses 10, 30 and 90 SID<sub>50</sub> were 52, 51 and 49 hpi and were not significantly different (p > 0.05). Shrimp orally inoculated with 10, 30 or 90 SID<sub>50</sub> developed disease between 24 and 36 hpi. Cumulative mortality was 100% at 108 hpi with doses of 30 and 90 SID<sub>50</sub> and 120 hpi with a dose of 10 SID<sub>50</sub>. The LT<sub>50</sub> of 10, 30 and 90 SID<sub>50</sub> were 65, 57 and 50 hpi; these were significantly different from each other (p < 0.05). A dose of 30 SID<sub>50</sub> was selected as the standard for further WSSV challenges by i.m. or oral routes. These standardized inoculation procedures may be applied to other crustacea and WSSV strains in order to achieve comparable results among experiments","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Litopenaeus vannamei; WSSV; experimental inoculation; intramuscular route; oral route LT<sub>50<; sub>; probit analysis","OtherDescriptors":null,"Notes":null,"AnaPub":2006,"MonPub":null,"DateUpdate":"2015-11-09","DateCreate":"2007-02-01","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000237303500001","VABBcode":null,"OpenAcc":1,"DOI":"10.3354/dao068181"},"refs":null,"anarec":{"AnaID":107286,"PubliDate":2006,"Pagination":"181-188","XtraPublOfAnaID":null,"ISBN":null,"Volume":"68","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42792,"SerRR":"Diseases of Aquatic Organisms. 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