{"refrec":{"BRefID":107414,"RR":"<b>Saroussi, S.; Beer, S.</b> (2007). Alpha and quantum yield of aquatic plants derived from PAM fluorometry: uses and misuses. <i>Aquat. Bot. 86(1)</i>: 89-92. <a href=\"http://dx.doi.org/10.1016/j.aquabot.2006.09.003\" target=\"_blank\">http://dx.doi.org/10.1016/j.aquabot.2006.09.003</a>","BEntID":102251,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Bot. 86(1)</i>: 89-92. <a href=\"https://dx.doi.org/10.1016/j.aquabot.2006.09.003\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquabot.2006.09.003</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Saroussi, S.; Beer, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Saroussi, S.; Beer, S.","Englishabstract":"The slope of the initial linear range of a photosynthesis–irradiance (<i>P</i>–<i>I</i>) curve, alpha (<i>a</i>), is frequently, but often incorrectly, used to denote the maximal quantum yield (or the “efficiency” of photosynthesis) of higher plants and macroalgae under the conditions for which the <i>P</i>–<i>I</i> curve was measured. When using the increasingly popular method of pulse amplitude modulated (PAM) fluorometry, the determination of <i>a</i> from so-called rapid light curves (RLC) may lead to misinterpretations when comparing photosynthetic efficiencies under different environmental conditions. Furthermore, since PAM fluorometry measures the quantum yield (<i>Y</i>) directly, there may be no need to estimate it from the initial slopes of RLCs.<p>We compared photosynthetic parameters derived from RLCs of <i>Ulva</i> sp. measured during winter and summer, and show large differences in <i>a</i> when electron transport rates (ETR) were plotted against incident irradiance (<i>I</i><sub>i</sub>) [<i>a</i> = 0.26 ± 0.00 versus 0.08 ± 0.01 during the winter (November–December) and summer (July–August), respectively], as is usually done. On the other hand, no differences in the initial slopes of the RLCs were apparent when plotting ETR versus the absorbed irradiance (<i>I</i><sub>a</sub>) (initial slope = 0.75 ± 0.01 versus 0.62 ± 0.12 during the winter and summer, respectively); this is called for since also ETR is calculated using <i>I</i><sub>a</sub>. Using the <i>I</i><sub>a</sub> based RLCs, it was also found that the values of the initial slopes equalled those of the first <i>Y</i>-value measurements of the RLCs (<i>Y</i><sub>0</sub>) (<i>t</i>-test, <i>p</i> > 0.05, <i>r</i><sup>2</sup> = 0.85). Therefore, when using PAM fluorometry, we suggest (a) to present the <i>x</i>-axis of RLCs as <i>I</i><sub>a</sub> (<i>I</i><sub>i</sub> × AF × 0.5), and ETR on the <i>y</i>-axis as <i>Y</i> × <i>I</i><sub>a</sub>, and (b) that <i>Y</i><sub>0</sub> can be taken as a correct measure of the maximal quantum yield instead of estimating it from an RLC.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Alpha and quantum yield of aquatic plants derived from PAM fluorometry: uses and misuses","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:32:52.928458","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"alpha; initial slope; PAM fluorometry; P-I curve; quantum yield; rapidlight curve; Ulva sp.","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2007-02-06","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000243613200014","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquabot.2006.09.003"},"refs":null,"anarec":{"AnaID":107414,"PubliDate":2007,"Pagination":"89-92","XtraPublOfAnaID":null,"ISBN":null,"Volume":"86","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42195,"SerRR":"Aquatic Botany. Elsevier Science: Tokyo; Oxford; New York; London; Amsterdam.  ISSN 0304-3770; e-ISSN 1879-1522","StandardTitleSer":"Aquatic Botany","ISSN":"0304-3770","AbbrevSer":"Aquat. 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