{"refrec":{"BRefID":107495,"RR":"<b>Papakostas, S.; Dooms, S.; Christodoulou, M.; Triantafyllidis, A.; Kappas, I.; Dierckens, K.; Bossier, P.; Sorgeloos, P.; Abatzopoulos, T.J.</b> (2006). Identification of cultured <i>Brachionus</i> rotifers based on RFLP and SSCP screening. <i>Mar. Biotechnol. 8(5)</i>: 547-559. <a href=\"http://dx.doi.org/10.1007/s10126-005-6181-z\" target=\"_blank\">dx.doi.org/10.1007/s10126-005-6181-z</a>","BEntID":102323,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biotechnol. 8(5)</i>: 547-559. <a href=\"https://dx.doi.org/10.1007/s10126-005-6181-z\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-005-6181-z</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Papakostas, S.; Dooms, S.; Christodoulou, M.; Triantafyllidis, A.; Kappas, I.; Dierckens, K.; Bossier, P.; Sorgeloos, P.; Abatzopoulos, T.J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Papakostas, S. <i>et al.</i>","Englishabstract":"The marine finfish industry worldwide depends greatly on the mass culture of <i>Brachionus</i> rotifers. Recently, molecular data have revealed a more complicated view about the species status of <i>Brachionus</i> rotifers than previous mainly morphological assessments. Under this view, <i>Brachionus</i> rotifers are comprised of many morphologically similar, albeit genetically differentiated, cryptic members of larger groups. A redefinition of the cultured rotifer species/biotypes is therefore needed if aquaculture is to reach higher levels of standardization and predictability. In this work, restriction fragment length polymorphism (RFLP) and single-strand conformational polymorphism (SSCP) methods are applied to the COI and 16S rRNA mitochondrial genes. A detailed COI restriction map was constructed, using sequence data from all known representatives of <i>Brachionus</i> phylogroups. Therefore, it is the first time that such an extended restriction database has been produced. Several restriction endonucleases are proposed for the discrimination of the different <i>Brachionus</i> species/biotypes. Furthermore, eight different SSCP gel alleles are described for the 16S region. Using these data, five <i>Brachionus</i> species/biotypes were identified in 78 samples collected from laboratories and hatcheries around the world.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Identification of cultured <i>Brachionus</i> rotifers based on RFLP and SSCP screening","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"COI restriction map; hatchery strains; rotifer clones; mitochondrial DNA; 16S rRNA","OtherDescriptors":null,"Notes":null,"AnaPub":2006,"MonPub":null,"DateUpdate":"2012-06-07","DateCreate":"2007-02-08","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000241558700011","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10126-005-6181-z"},"refs":null,"anarec":{"AnaID":107495,"PubliDate":2006,"Pagination":"547-559","XtraPublOfAnaID":null,"ISBN":null,"Volume":"8","Issue":"5","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45426,"SerRR":"Marine Biotechnology. Springer-Verlag: New York.  ISSN 1436-2228; e-ISSN 1436-2236","StandardTitleSer":"Marine Biotechnology","ISSN":"1436-2228","AbbrevSer":"Mar. Biotechnol.","StandardTitleMon":null,"StartPage":547,"Pages":13,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Papakostas","Firstname":"Spiros","Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":102323,"AutID":70211,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Dooms","Firstname":"Stefania","Initials":"S.","Affiliation":"Laboratory of Aquaculture and Artemia Reference Center, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":102323,"AutID":148286,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":null,"PersID":3608,"InsID":22},{"AutName":"Christodoulou","Firstname":"Marianna","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":102323,"AutID":71607,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Triantafyllidis","Firstname":"Alexander","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":102323,"AutID":40044,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Kappas","Firstname":"Ilias","Initials":"I.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":102323,"AutID":26369,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Dierckens","Firstname":"Kristof","Initials":"K.","Affiliation":"Laboratory of Aquaculture and Artemia Reference Center, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":102323,"AutID":144375,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":null,"PersID":2557,"InsID":22},{"AutName":"Bossier","Firstname":"Peter","Initials":"P.","Affiliation":"Laboratory of Aquaculture and Artemia Reference Center, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":102323,"AutID":144305,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":"0000-0002-6165-9111","PersID":125,"InsID":22},{"AutName":"Sorgeloos","Firstname":"Patrick","Initials":"P.","Affiliation":"Laboratory of Aquaculture and Artemia Reference Center, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":102323,"AutID":143941,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":null,"PersID":35,"InsID":22},{"AutName":"Abatzopoulos","Firstname":"Theodore","Initials":"T.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":102323,"AutID":558,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":115402,"LibID":36,"BRefID":107495,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"115402","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Springer-Verlag","City":"New York"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTermPath":"Acids > Organic compounds > Organic acids > Nucleic acids > DNA"},{"ThesaurusTerm":"Hatcheries","ThestID":3916,"Acronym":"ASFA","ThesTermPath":"Aquaculture facilities > Hatcheries"},{"ThesaurusTerm":"Mitochondria","ThestID":9650,"Acronym":"ASFA","ThesTermPath":"Cell constituents > Cell organelles > Mitochondria"}],"thestermsASFA":[{"ThesaurusTerm":"DNA"},{"ThesaurusTerm":"Hatcheries"},{"ThesaurusTerm":"Mitochondria"}],"taxtermsASFA":[{"TaxTerm":"Rotifera"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":102323,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":102323,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/s10126-005-6181-z","externalID":"10.1007/s10126-005-6181-z","URLTypeCode":"DOI","URLID":6162,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Hatcheries","ThestID":3916,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Mitochondria","ThestID":9650,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Rotifera","AphiaID":14260,"TaxtID":16699}],"geoterms":null,"othterms":[{"OtherTerm":"16s rDNA","OthtID":10611},{"OtherTerm":"Rotifier clones","OthtID":16395}],"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"DNA","DutchTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Hatcheries","DutchTerm":null,"ThestID":3916,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Mitochondria","DutchTerm":null,"ThestID":9650,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Rotifera","DutchTerm":"Raderdiertjes","AphiaID":14260,"TaxtID":16699}],"geotermsFRIS":null,"othtermsFRIS":[{"OtherTerm":"16s rDNA","DutchTerm":null,"OthtID":10611},{"OtherTerm":"Rotifier clones","DutchTerm":null,"OthtID":16395}],"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2007-02-08 09:11:08.400000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2012-06-07 09:44:03.450000","timezone_type":3,"timezone":"Europe/Brussels"}}}