{"refrec":{"BRefID":107525,"RR":"<b>Ghekiere, A.; Fenske, M.; Verslycke, T.; Tyler, C.; Janssen, C.R.<\/b> (2005). Development of a quantitative enzyme-linked immunosorbent assay for vitellin in the mysid <i>Neomysis integer<\/i> (Crustacea: Mysidacea). <i>Comp. Biochem. Physiol., Part A Physiol. 142(1)<\/i>: 43-49. <a href=\"http:\/\/dx.doi.org\/10.1016\/j.cbpa.2005.07.006\" target=\"_blank\">dx.doi.org\/10.1016\/j.cbpa.2005.07.006<\/a>","BEntID":102352,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Comp. Biochem. Physiol., Part A Physiol. 142(1)<\/i>: 43-49. <a href=\"https:\/\/dx.doi.org\/10.1016\/j.cbpa.2005.07.006\" target=\"_blank\">https:\/\/dx.doi.org\/10.1016\/j.cbpa.2005.07.006<\/a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Ghekiere, A.; Fenske, M.; Verslycke, T.; Tyler, C.; Janssen, C.R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ghekiere, A. <i>et al.<\/i>","Englishabstract":"Mysid crustaceans have been put forward by several regulatory bodies as suitable test organisms to screen and test the potential effects of environmental endocrine disruptors. Despite the well-established use of mysid reproductive endpoints such as fecundity, egg development time, and time to first brood release in standard toxicity testing, little information exists on the hormonal regulation of these processes. Control of vitellogenesis is being studied intensively because yolk is an excellent model for studying mechanisms of hormonal control, and vitellogenesis can be chemically disrupted. Yolk protein or vitellin is a major source of nourishment during embryonic development of ovigorous egg-laying invertebrates. The accumulation of vitellin during oocyte development is vital for the production of viable offspring. In this context, we developed a competitive enzyme-linked immunosorbent assay (ELISA) for vitellin of the estuarine mysid <i>Neomysis integer<\/i>. Mysid vitellin was isolated using gel filtration, and the purified vitellin was used to raise polyclonal antibodies. The ELISA was sensitive within a working range of 4 to 500 ng vitellin\/mL. Serial dilutions of whole body homogenates from female <i>N. integer<\/i> and the vitellin standard showed parallel binding curves, validating the specificity of the ELISA. The intra- and interassay coefficients of variation were 8.2% and 13.8%, respectively. Mysid vitellin concentrations were determined from ovigorous females and eggs at different developmental stages. The availability of a quantitative mysid vitellin ELISA should stimulate further studies on the basic biology of this process in mysids. Furthermore, it could provide a means to better understand and predict chemically induced reproductive effects in mysids.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Development of a quantitative enzyme-linked immunosorbent assay for vitellin in the mysid <i>Neomysis integer<\/i> (Crustacea: Mysidacea)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Crustacea; endocrine disruption; ELISA; mysids; Neomysis integer;vitellin; vitellogenesis","OtherDescriptors":null,"Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2016-10-09","DateCreate":"2007-02-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000232327700005","VABBcode":null,"OpenAcc":0,"DOI":"10.1016\/j.cbpa.2005.07.006"},"refs":null,"anarec":{"AnaID":107525,"PubliDate":2005,"Pagination":"43-49","XtraPublOfAnaID":null,"ISBN":null,"Volume":"142","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42580,"SerRR":"Comparative Biochemistry and Physiology. Part A. Physiology. Pergamon: Oxford.  ISSN 0300-9629; e-ISSN 1531-4332","StandardTitleSer":"Comparative Biochemistry and Physiology. Part A. Physiology","ISSN":"0300-9629","AbbrevSer":"Comp. Biochem. Physiol., Part A Physiol.","StandardTitleMon":null,"StartPage":43,"Pages":7,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Ghekiere","Firstname":"An","Initials":"A.","Affiliation":"Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":102352,"AutID":144246,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"GhEnToxLab","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Milieutoxicologie","ORCID":null,"PersID":4325,"InsID":6705},{"AutName":"Fenske","Firstname":null,"Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":102352,"AutID":7071,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Verslycke","Firstname":"Tim","Initials":"T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":102352,"AutID":3575,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":255,"InsID":null},{"AutName":"Tyler","Firstname":"Charles","Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":102352,"AutID":71646,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Janssen","Firstname":"Colin","Initials":"C.R.","Affiliation":"Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":102352,"AutID":144240,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"GhEnToxLab","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Milieutoxicologie","ORCID":"0000-0002-7781-6679","PersID":50,"InsID":6705}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":115441,"LibID":36,"BRefID":107525,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"115441","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https:\/\/www.vliz.be\/imisdocs\/publications\/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Pergamon","City":"Oxford"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Endocrine glands","ThestID":2767,"Acronym":"ASFA","ThesTermPath":"Secretory organs > Glands > Endocrine glands"},{"ThesaurusTerm":"Vitellogenesis","ThestID":9020,"Acronym":"ASFA","ThesTermPath":"Vitellogenesis"}],"thestermsASFA":[{"ThesaurusTerm":"Endocrine glands"},{"ThesaurusTerm":"Vitellogenesis"}],"taxtermsASFA":[{"TaxTerm":"Neomysis integer (Leach, 1814)"}],"geotermsASFA":[{"GeoTerm":"Galgenweel"}],"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"},{"Collection":"ScheldeMonitor","ShortName":"scheldemonitor"},{"Collection":"Section Ecotox","ShortName":"Ecotox"}],"conf":null,"proj":[{"ProID":1068,"Acronym":"ENDIS-RISKS","ProStandardTitle":"Endocrine disruption in the Scheldt Estuary: distribution, exposure and effects","BYear":2002,"BMonth":2,"EYear":2006,"EMonth":4,"FP7Code":null,"RelID":null,"Relation":null,"DutchTerm":null}],"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"36":{"SpName":"Endis-Risks","SpColID":36,"ParSpColID":null,"TopParID":null,"ShortName":"endisrisks","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"endisrisks"},"867":{"SpName":"IMIS themes","SpColID":867,"ParSpColID":null,"TopParID":null,"ShortName":"IMIS themes","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"IMIS themes"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"},"27":{"SpName":"ScheldeMonitor","SpColID":27,"ParSpColID":null,"TopParID":null,"ShortName":"scheldemonitor","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"scheldemonitor"},"476":{"SpName":"Section Ecotox","SpColID":476,"ParSpColID":null,"TopParID":null,"ShortName":"Ecotox","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"Ecotox"},"641":{"SpName":"Species diversity","SpColID":641,"ParSpColID":867,"TopParID":867,"ShortName":"Diversiteit soorten","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":867,"SpColPath":"IMIS themes\/Diversiteit soorten"},"664":{"SpName":"Species diversity: Benthos","SpColID":664,"ParSpColID":641,"TopParID":867,"ShortName":"Diversiteit soorten: Benthos","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":867,"SpColPath":"IMIS themes\/Diversiteit soorten\/Diversiteit soorten: Benthos"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":102352,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":102352,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https:\/\/dx.doi.org\/10.1016\/j.cbpa.2005.07.006","externalID":"10.1016\/j.cbpa.2005.07.006","URLTypeCode":"DOI","URLID":51418,"URLTypID":13,"URLType":"DOI","URLPrefix":"http:\/\/dx.doi.org\/"}],"thesterms":[{"ThesaurusTerm":"Endocrine glands","ThestID":2767,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Vitellogenesis","ThestID":9020,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Neomysis integer (Leach, 1814) [An opossum shrimp]","AphiaID":120136,"TaxtID":16803}],"geoterms":[{"GeoTerm":"Galgenweel","GeoObjectID":9114,"GeotID":6791}],"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Endocrine glands","DutchTerm":"Endocriene klieren","ThestID":2767,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Vitellogenesis","DutchTerm":"Vitellogenesis","ThestID":9020,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Neomysis integer (Leach, 1814)","DutchTerm":"Brakwateraasgarnaal","AphiaID":120136,"TaxtID":16803}],"geotermsFRIS":[{"GeoTerm":"Galgenweel","DutchTerm":null}],"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Haspeslagh, Jan, J.","newSesDate":{"date":"2007-02-09 10:15:39.470000","timezone_type":3,"timezone":"Europe\/Brussels"},"updSesName":"Lyssens, Liesbeth, L.","updSesDate":{"date":"2016-10-09 21:37:27.480000","timezone_type":3,"timezone":"Europe\/Brussels"}}}