{"refrec":{"BRefID":108056,"RR":"<b>Boskou, G.; Debevere, J.</b> (2000). Shelf-life extension of cod fillets with an acetate buffer spray prior to packaging under modified atmospheres. <i>Food Addit. Contam. 17(1)</i>: 17-25","BEntID":102848,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":1,"RefStringPartII":". <i>Food Addit. Contam. 17(1)</i>: 17-25","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Boskou, G.; Debevere, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Boskou, G.; Debevere, J.","Englishabstract":"Fresh cod fillets (<i>Gadus morhua</i>) were sprayed with a 10% acetate buffer (pH 5.6), packed with an industrial gas-flushing packaging machine under modified atmospheres (50% CO2-45% O2-5% N2, 2cm3/1g gas/ product ratio) and stored at 7oC for 12 days. Control cod fillets were directly packed and stored under the same conditions. A reduction of the aerobic plate counts was observed immediately after the cod fillets had been sprayed. During storage under modified atmospheres, there was complete inhibition of H2S-producing bacteria and Enterobacteriaceae in the treated cod fillets. Production of total volatile bases and trimethylamine (TMA) was inhibited in treated fillets for 10 days' storage under modified atmospheres. Inhibition of TMA production can be attributed to growth inhibition of H2S-producing bacteria, inhibition of the trimethylamine oxide (TMAO)-dependent metabolism of TMAO-reducing bacteria and the stable pH during storage. 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