{"refrec":{"BRefID":110014,"RR":"Yuen, B.B.H.; Wong, C.K.C.; Woo, N.Y.S.; Au, D.W.T. (2007). Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene. Aquat. Toxicol. 82(3): 181-194. https://dx.doi.org/10.1016/j.aquatox.2007.02.010","BEntID":104699,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". Aquat. Toxicol. 82(3): 181-194. https://dx.doi.org/10.1016/j.aquatox.2007.02.010","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Yuen, B.B.H.; Wong, C.K.C.; Woo, N.Y.S.; Au, D.W.T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Yuen, B.B.H. et al.","Englishabstract":"The sublethal toxicity of dietary benzo[a]pyrene, B[a]P, on fish growth and intestinal morphofunctional changes [as measured by epithelial turnover, cell proliferation, hyperplasia, de novo crypt formation and protein absorption efficiency (i.e. expression of proton/peptide co-transporter, PepT-1, on the mucosal brush border)] were studied for the carnivorous orange-spotted grouper (Epinephelus coioides). Juvenile fish were force-fed daily with pellets containing environmentally realistic concentrations of B[a]P (dissolved in corn oil) at 0.25 μg/g body weight (low-dose) and 12.5 μg/g body weight (high-dose) for 4 weeks, followed by a control diet for a further 4 weeks to assess recovery. Although growth inhibition was observed in fish treated with high-dose B[a]P during the exposure period, no mortality was observed throughout the 8-week experiment. Significant hyperplasia of basal enterocytes of mucosal folds was detected shortly after 3-day exposure to the high-dose B[a]P. Moreover, a faster epithelial turnover was measured in the high-dose B[a]P exposed fish at exposure week 1, which was followed by an increase of basal cell proliferation and a reduction of PepT-1 expression at exposure week 2. The formation of de novo crypts, resemblance to the cancer predisposition syndrome “juvenile polyposis”, was significantly higher in the intestine of high-dose treated fish as compared to the control at exposure week 2 and onwards. Abnormal cytoplasmic extrusions were frequently observed in mucosal folds of high-dose fish at exposure week 4. In the low-dose treatment group, only the expression of PepT-1 was significantly reduced at exposure week 2 and an early adaptive response was observed at exposure week 4. Despite all these intestinal disturbances were reversible in fish upon the abatement to dietary B[a]P (within 1–4 weeks), environmental realistic levels of foodborne B[a]P could induce sublethal toxicity to E. coioides, and probably impose potential risk to the marine environment. As an increase in de novo crypts was observed towards the end of the 4-week depuration period, the long-term impacts of dietary B[a]P on fish intestinal neoplasm formation worth further investigation.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-14 01:31:14.250473","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"dietary B[a]P; intestine; histopathology; immunohistochemistry; recovery","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2020-10-22","DateCreate":"2007-04-30","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000246138800004","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2007.02.010"},"refs":null,"anarec":{"AnaID":110014,"PubliDate":2007,"Pagination":"181-194","XtraPublOfAnaID":null,"ISBN":null,"Volume":"82","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":181,"Pages":14,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"Department of Biology and Chemistry","Email":"bhdwtau@city.edu.hk","Line2":"City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong","EnvName":"China"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Yuen","Firstname":"Bonny","Initials":"B.B.H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":104699,"AutID":73175,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Wong","Firstname":"Chris","Initials":"C.K.C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":104699,"AutID":73176,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Woo","Firstname":"Norman","Initials":"N.Y.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":104699,"AutID":72030,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Au","Firstname":"Doris","Initials":"D.W.T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":104699,"AutID":52759,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":353141,"LibID":36,"BRefID":110014,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"353141","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
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