Document of bibliographic reference 110661

BibliographicReference record

Type

Bibliographic resource

Type of document

Book chapters

BibLvlCode

AM

Title

Purification and characterization of vitellin from the estuarine mysid Neomysis integer (Crustacea: Mysidacea)

Abstract

Invertebrates account for roughly 95% of all animals, yet surprisingly, little effort has been invested to understand their value in signaling potential environmental endocrine disruption. There has been, however, much recent attention on vitellogenin induction in egg-laying invertebrates and vertebrates as indicators of exposure to estrogenic xenobiotics. Mysid shrimp (Crustacea: Mysidacea) have been put forward by several researchers and regulatory bodies (e.g., US-EPA) as suitable test organisms for the evaluation of environmental endocrine disruption. In view of developing sensitive assays to study endocrine disruption in the estuarine mysid Neomysis integer, we isolated and characterized vitellin, the major yolk protein in eggs. Vitellin was purified using gel filtration and characterized by electrophoresis using different staining procedures. Specific (as shown by Western blotting) polyclonal antibodies were produced in rabbit against the purified vitellin of N. integer. These antisera will be used to develop immunoassays to study vitellogenesis in mysids and to detect potential stimulatory or inhibitory effects of endocrine disruptors on the production of vitellin.

Bibliographic citation

Ghekiere, A.; Verslycke, T.; De Smet, L.; Van Beeumen, J.; Janssen, C.R. (2006). Purification and characterization of vitellin from the estuarine mysid Neomysis integer (Crustacea: Mysidacea), in: Ghekiere, A. Studie van invertebraat-specifieke effecten van endocrien-verstorende stoffen in de estuariene aasgarnaal Neomysis integer (Leach, 1814) = Study of invertebrate-specific effects of endocrine disrupting chemicals in the estuarine mysid Neomysis integer (Leach, 1814). pp. 24-35

Topic

Brakish water