{"refrec":{"BRefID":110776,"RR":"<b>Andersson, C.; Katsiadaki, I.; Lundstedt-Enkel, K.; Örberg, J.</b> (2007). Effects of 17α-ethynylestradiol on EROD activity, spiggin and vitellogenin in three-spined stickleback (<i>Gasterosteus aculeatus</i>). <i>Aquat. Toxicol. 83(1)</i>: 33-42. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2007.03.008\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2007.03.008</a>","BEntID":105421,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Toxicol. 83(1)</i>: 33-42. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2007.03.008\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2007.03.008</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Andersson, C.; Katsiadaki, I.; Lundstedt-Enkel, K.; Örberg, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Andersson, C. <i>et al.</i>","Englishabstract":"The three-spined stickleback (<i>Gasterosteus aculeatus</i>) has quantifiable biomarkers of exposure to estrogens (vitellogenin), androgens (spiggin) and aryl hydrocarbon receptor (AhR) agonists (EROD activity) and is therefore a promising test species for biomonitoring of reprotoxic chemicals in aquatic environments. In this study we evaluated the effects of 17α-ethynylestradiol (EE<sub>2</sub>) on EROD activity, induction of vitellogenin and spiggin, hepatosomatic index (HSI), ovarian somatic index (OSI) and nephrosomatic index (NSI). Adult male and female three-spined sticklebacks were exposed to concentrations of 0–170 ng EE<sub>2</sub>/l (measured concentrations) in a flow-through system for 21 days. Exposure to 170 ng EE<sub>2</sub>/l resulted in a significant 8- and 9-fold induction of gill EROD activity in males and females, respectively. In livers, EROD activity expressed in relation to microsomal protein content was suppressed due to a significant increase in microsomal protein content. Hepatic EROD activity <i>per se</i> expressed as picomol/min was not affected by exposure to EE<sub>2</sub>. The lowest observed effect concentration for induction of vitellogenin in males was 53.7 ng EE<sub>2</sub>/l. In females, vitellogenin levels were significantly higher in those exposed to170 ng EE<sub>2</sub>/l compared to controls. Spiggin production was significantly inhibited and NSI lower in males exposed to 170 ng EE<sub>2</sub>/l. In both females and males LSI was significantly higher in fish exposed to 170 ng EE<sub>2</sub>/l than in controls. In females exposed to 170 ng EE<sub>2</sub>/l, OSI was significantly lower and NSI higher than controls. The observed results from this study show that a synthetic estrogen can affect the well-known biomarker of exposure for dioxin-like compounds, EROD activity, and further that this response can differ between tissues. These findings are important for interpretation of biomonitoring data.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Effects of 17α-ethynylestradiol on EROD activity, spiggin and vitellogenin in three-spined stickleback (<i>Gasterosteus aculeatus</i>)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-14 01:31:14.375487","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"EROD activity; spiggin; vitellogenin; three-spined stickleback; 17alpha-ethynylestradiol","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2020-10-22","DateCreate":"2007-05-31","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000246952400004","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2007.03.008"},"refs":null,"anarec":{"AnaID":110776,"PubliDate":2007,"Pagination":"33-42","XtraPublOfAnaID":null,"ISBN":null,"Volume":"83","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. 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