{"refrec":{"BRefID":111734,"RR":"<b>Rhee, J.-S.; Lee, Y.-M.; Hwang, D.-S.; Won, E.-J.; Raisuddin, S.; Shin, K.-H.; Lee, J.-S.</b> (2007). Molecular cloning, expression, biochemical characteristics, and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete <i>Neanthes succinea</i>. <i>Aquat. Toxicol. 83(2)</i>: 104-115. <a href=\"http://dx.doi.org/10.1016/j.aquatox.2007.03.015\" target=\"_blank\">http://dx.doi.org/10.1016/j.aquatox.2007.03.015</a>","BEntID":106351,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 83(2)</i>: 104-115. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2007.03.015\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2007.03.015</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Rhee, J.-S.; Lee, Y.-M.; Hwang, D.-S.; Won, E.-J.; Raisuddin, S.; Shin, K.-H.; Lee, J.-S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Rhee, J.-S. <i>et al.</i>","Englishabstract":"We cloned and sequenced the full-length cDNA of a theta class glutathione S-transferase (GST-T) from the polychaete <i>Neanthes succinea</i>. The open reading frame of N. succinea GST-T cDNA was 678 bp and encoded 226 amino acid residues. We generated recombinant N. succinea GST-T by expression in transformed <i>Escherichia coli </i>and studied the kinetic properties as well as the effects of inhibitors, pH, and temperature on <i>N. succinea </i>GST-T. GST-T expression was studied using real-time RT-PCR in response to exposure to the model oxidative stress-inducing agent, CuCl2. Copper induced a concentration-dependant increase in the expression of GST-T. Moreover, polychaetes collected from a heavily contaminated lake near an industrial complex showed significantly higher levels of GST-T expression. Interestingly, the site-collected polychaetes with the highest GST-T mRNA expression levels also showed the highest metallothioneins levels. These results suggest that GST-T in polychaetes may have an antioxidant role and that <i>N. succinea </i>GST-T expression may be a useful biomarker for exposure to environmental contaminants such as copper. Our findings provide a better understanding of the biochemical characteristics of <i>N. succinea </i>GST-T, and elucidate the potential role of GST-T in heavy metal-induced oxidative stress and as a biomarker for environmental contamination.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Molecular cloning, expression, biochemical characteristics, and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete <i>Neanthes succinea</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"polychaete; neanthes succinea; glutathione S-transferases; heavy metals;metallothioneins; oxidative stress; biomarker","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2007-07-20","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000247556100003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2007.03.015"},"refs":null,"anarec":{"AnaID":111734,"PubliDate":2007,"Pagination":"104-115","XtraPublOfAnaID":null,"ISBN":null,"Volume":"83","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. 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