{"refrec":{"BRefID":111764,"RR":"<b>Middelboe, A.L.; Hansen, P.J.</b> (2007). Direct effects of pH and inorganic carbon on macroalgal photosynthesis and growth. <i>Mar. Biol. Res. 3(3)</i>: 134-144. <a href=\"http://dx.doi.org/10.1080/17451000701320556\" target=\"_blank\">http://dx.doi.org/10.1080/17451000701320556</a>","BEntID":106381,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biol. Res. 3(3)</i>: 134-144. <a href=\"https://dx.doi.org/10.1080/17451000701320556\" target=\"_blank\">https://dx.doi.org/10.1080/17451000701320556</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Middelboe, A.L.; Hansen, P.J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Middelboe, A.L.; Hansen, P.J.","Englishabstract":"The maximum final pHs reached during extended photosynthesis were 8.83 for the red algae <i>Delesseria sanguinea </i>and 10.19 for the brown algae <i>Fucus vesiculosus</i>, as long as the concentrations of [CO<sub>2</sub>+HCO<sub>3-</sub>] exceeded 1 mmol l-1, suggesting the existence of an upper pH limit for macroalgal photosynthesis. In experiments initiated with low concentrations of inorganic carbon, the final pH decreased with reduced initial pH, indicating that inorganic carbon became limiting. Effects of high O<sub>2 </sub>concentrations could be ruled out as a contributing factor in pH drift experiments initiated at or above pH 7.5. The growth rates of both species decreased when pH exceeded 8.5. However, although the growth of <i>D. sanguinea </i>stopped at pH 9.1-9.3, depending on acclimation, <i>F. vesiculosus </i>could maintain its growth up to a pH of 9.8 in these experiments. Additional experiments carried out on <i>F. vesiculosus </i>at two pH levels, 8 and 9.3, and a range of dissolved inorganic carbon concentrations revealed that the growth of <i>F. vesiculosus </i>was reduced at pH 9.3 compared with pH 8, irrespectively of inorganic carbon concentration. 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