{"refrec":{"BRefID":112117,"RR":"<b>Cozza, R.; Pangaro, T.; Maestrini, P.; Giordani, T.; Natali, L.; Cavallini, A.</b> (2006). Isolation of putative type 2 metallothionein encoding sequences and spatial expression pattern in the seagrass <i>Posidonia oceanica</i>. <i>Aquat. Bot. 85(4)</i>: 317-323. <a href=\"https://dx.doi.org/10.1016/j.aquabot.2006.06.010\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquabot.2006.06.010</a>","BEntID":106708,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Bot. 85(4)</i>: 317-323. <a href=\"https://dx.doi.org/10.1016/j.aquabot.2006.06.010\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquabot.2006.06.010</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Cozza, R.; Pangaro, T.; Maestrini, P.; Giordani, T.; Natali, L.; Cavallini, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Cozza, R. <i>et al.</i>","Englishabstract":"Metallothioneins (MTs) are a group of proteins with low molecular mass and high cysteine content that bind to heavy metals and are thought to play a role in their metabolism and detoxification. Genes encoding MT-like proteins have been isolated in a number of plants. In this work we isolated nine MT-like sequences from copper- or cadmium-exposed plants of the seagrass <i>Posidonia oceanica</i>, a marine Angiosperm playing a major role in maintaining infralittoral ecosystems in the Mediterranean sea. These sequences, together with two other MT genes previously isolated from this species, show high similarities with genes encoding type 2 MTs. Neighbour-joining analysis, at both deduced protein and 3'-UTR sequence level, indicates that at least two subgroups occur within <i>Posidonia</i> type 2 MTs, showing, however, a strong sequence uniformity. Southern analysis of two type 2 MT-encoding sequences (<i>Pomt2b </i>and <i>Pomt2f</i>) belonging to the two different subgroups showed distinct hybridisation patterns. For both type 2 MTs, we have determined, by in situ technique, the expression domain in <i>Posidonia</i> plants. The members of these two MT subgroups show differences in their histological expression, with <i>Pomt2b</i> associated with proliferative tissues whereas <i>Pomt2f</i> is associated with lignified or suberized cell wall. 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