{"refrec":{"BRefID":112308,"RR":"<b>Chen, S.-L.; Sha, Z.X.; Ye, H.-Q.; Liu, Y.; Tian, Y.-S.; Hong, Y.; Tang, Q.-S.</b> (2007). Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (<i>Lateolabrax japonicus</i>). <i>Mar. Biotechnol. 9(1)</i>: 82-91. <a href=\"http://dx.doi.org/10.1007/s10126-006-6050-1\" target=\"_blank\">http://dx.doi.org/10.1007/s10126-006-6050-1</a>","BEntID":106880,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biotechnol. 9(1)</i>: 82-91. <a href=\"https://dx.doi.org/10.1007/s10126-006-6050-1\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-006-6050-1</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Chen, S.-L.; Sha, Z.X.; Ye, H.-Q.; Liu, Y.; Tian, Y.-S.; Hong, Y.; Tang, Q.-S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Chen, S.-L. <i>et al.</i>","Englishabstract":"A stable GFP-expressing (GFP<sup>+</sup>LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (<i>Lateolabrax japonicus</i>,) embryos. GFP<sup>+</sup>LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP<sup>+</sup>LJES1 cells were transplanted into sea perch and zebrafish embryos at mid-blastula stage. Twenty out of 478 transplanted sea perch embryos contained GFP-expressing LJES1 cells 24 h after microinjection. Fifteen chimera embryos developed into fry. In these chimeras, the GFP<sup>+</sup>LJES1 cells contributed to a variety of tissues including the head and trunk. In zebrafish, 221 embryos were microinjected with GFP<sup>+</sup>LJES1 cells and 22 chimera embryos and fries expressing GFP were obtained. Donor GFP<sup>+</sup>LJES1 cells contributed to various tissues in head and trunk of zebrafish embryos and hatched fry. 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