{"refrec":{"BRefID":112313,"RR":"<b>Yoshizaki, A.G.; Kiron, V.; Satoh, S.; Takeuchi, T.</b> (2007). Expression of masu salmon Δ5-desaturase-like gene elevated EPA and DHA biosynthesis in zebrafish. <i>Mar. Biotechnol. 9(1)</i>: 92-100. <a href=\"http://dx.doi.org/10.1007/s10126-006-6003-y\" target=\"_blank\">http://dx.doi.org/10.1007/s10126-006-6003-y</a>","BEntID":106885,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biotechnol. 9(1)</i>: 92-100. <a href=\"https://dx.doi.org/10.1007/s10126-006-6003-y\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-006-6003-y</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Yoshizaki, A.G.; Kiron, V.; Satoh, S.; Takeuchi, T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Yoshizaki, A.G. <i>et al.</i>","Englishabstract":"Farmed fish could substitute for marine capture fish as a source of fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) beneficial for human health; however, they require these compounds in their diets. In the present study on a model fish species, we modified the EPA/DHA biosynthesis pathway by overexpression of masu salmon Δ5-desaturase-like gene in zebrafish to increase its ability to synthesize EPA and DHA. Expression of this gene in transgenic fish fed a commercial diet and <i>Artemia</i> helped to improve their EPA content by 1.21-fold and DHA by 1.24-fold. In similar fish that were fed only <i>Artemia</i> the increments were 1.14-fold for EPA and 1.13-fold for DHA, compared with nontransgenic fish. In contrast, eicosatetraenoic acid content decreased, as it is a substrate of Δ5-desaturase, while the total lipid remained constant. The results demonstrated that masu salmon Δ5-desaturase is functional in zebrafish and can modify its fatty acid metabolic pathway. The technique could be applied to farmed fish to generate a nutritionally richer product for human consumption. 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