{"refrec":{"BRefID":112318,"RR":"<b>Yoshino, F.; Ikeda, H.; Masukawa, H.; Sakurai, H.</b> (2007). High photobiological hydrogen production activity of a <i>Nostoc</i> sp. PCC 7422 uptake hydrogenase-deficient mutant with high nitrogenase activity. <i>Mar. Biotechnol. 9(1)</i>: 101-112. <a href=\"http://dx.doi.org/10.1007/s10126-006-6035-3\" target=\"_blank\">http://dx.doi.org/10.1007/s10126-006-6035-3</a>","BEntID":106891,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biotechnol. 9(1)</i>: 101-112. <a href=\"https://dx.doi.org/10.1007/s10126-006-6035-3\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-006-6035-3</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Yoshino, F.; Ikeda, H.; Masukawa, H.; Sakurai, H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Yoshino, F. <i>et al.</i>","Englishabstract":"We describe a strategy to establish cyanobacterial strains with high levels of H2 production that involves the identification of promising wild-type strains followed by optimization of the selected strains using genetic engineering. <i>Nostoc</i> sp. PCC 7422 was chosen from 12 other heterocystous strains, because it has the highest nitrogenase activity. We sequenced the uptake hydrogenase (Hup) gene cluster as well as the bidirectional hydrogenase gene cluster from the strain, and constructed a mutant (Δ<i>hupL</i>) by insertional disruption of the hupL gene. The Δ<i>hupL</i> mutant produced H<sub>2</sub> at 100 μmoles mg chlorophyll a<sup>-1 </sup>h<sup>-1</sup>, a rate three times that of the wild-type. The Δ<i>hupL</i> cells could accumulate H2 to about 29% (v/v) accompanied by O<sub>2</sub> evolution in 6 days, under a starting gas phase of Ar + 5% CO<sub>2</sub>. The presence of 20% O<sub>2</sub> in the initial gas phase inhibited H<sub>2</sub> accumulation of the Δ<i>hupL</i> cells by less than 20% until day 7. ","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"High photobiological hydrogen production activity of a <i>Nostoc</i> sp. 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