{"refrec":{"BRefID":112888,"RR":"Parameswaran, V.; Ahmed, V.P.I.; Shukla, R.; Bhonde, R.R.; Hameed, A.S.S. (2007). Development and characterization of two new cell lines from milkfish (Chanos chanos) and grouper (Epinephelus coioides) for virus isolation. Mar. Biotechnol. 9(2): 281-291. https://dx.doi.org/10.1007/s10126-006-6110-9","BEntID":107452,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". Mar. Biotechnol. 9(2): 281-291. https://dx.doi.org/10.1007/s10126-006-6110-9","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Parameswaran, V.; Ahmed, V.P.I.; Shukla, R.; Bhonde, R.R.; Hameed, A.S.S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Parameswaran, V. et al.","Englishabstract":"Two new cell lines, SIMH and SIGE, were derived from the heart of milkfish (Chanos chanos), a euryhaline teleost, and from the eye of grouper (Epinephelus coioides), respectively. These cell lines were maintained in Leibovitz’s L-15 supplemented with 20% fetal bovine serum (FBS). The SIMH cell line was subcultured more than 50 times over a period of 210 days and SIGE cell line has been subcultured 100 times over a period of 1 1/2 years. The SIMH cell line consists predominantly of fibroblastic-like cells. The SIGE cell line consists predominantly of epithelial cells. Both the cell lines were able to grow at temperatures between 25 and 32°C with an optimum temperature of 28°C. The growth rate of these cells increased as the proportion of FBS increased from 2% to 20% at 28°C with optimum growth at the concentrations of 15% or 20% FBS. Seven marine fish viruses were tested to determine the susceptibility of these cell lines. The SIGE cell line was found to be susceptible to nodavirus, MABV NC-1 and Y6, and the infection was confirmed by cytopathic effect (CPE) and reverse transcriptase-polymerase chain reaction. When these cells were transfected with pEGFP-N1 vector DNA, significant fluorescent signals were observed, suggesting that these cell lines can be a useful tool for transgenic and genetic manipulation studies. Further, these cell lines are characterized by immunocytochemistry usingconfocal laser scanning microscopy (CFLSM).","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Development and characterization of two new cell lines from milkfish (Chanos chanos) and grouper (Epinephelus coioides) for virus isolation","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-14 01:31:14.625506","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"cell line; Chanos chanos; Epinephelus coioides; eye; heart; virus","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2020-10-22","DateCreate":"2007-08-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000245949800014","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10126-006-6110-9"},"refs":null,"anarec":{"AnaID":112888,"PubliDate":2007,"Pagination":"281-291","XtraPublOfAnaID":null,"ISBN":null,"Volume":"9","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45426,"SerRR":"Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236","StandardTitleSer":"Marine Biotechnology","ISSN":"1436-2228","AbbrevSer":"Mar. Biotechnol.","StandardTitleMon":null,"StartPage":281,"Pages":11,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Parameswaran","Firstname":null,"Initials":"V.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":107452,"AutID":75236,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Ahmed","Firstname":"V. P. Ishaq","Initials":"V.P.I.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":107452,"AutID":75237,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Shukla","Firstname":"Ravi","Initials":"R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":107452,"AutID":75238,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bhonde","Firstname":null,"Initials":"R.R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":107452,"AutID":75239,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Hameed","Firstname":"A.S. 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