{"refrec":{"BRefID":113519,"RR":"<b>Zhou, G.-Z.; Li, Z.-Q.; Yuan, X.-P.; Zhang, Q.-Y.</b> (2007). Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (<i>Epinephelus akaara</i>). <i>Mar. Biotechnol. 9(3)</i>: 370-376. <a href=\"https://dx.doi.org/10.1007/s10126-006-7165-3\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-006-7165-3</a>","BEntID":107975,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Biotechnol. 9(3)</i>: 370-376. <a href=\"https://dx.doi.org/10.1007/s10126-006-7165-3\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-006-7165-3</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Zhou, G.-Z.; Li, Z.-Q.; Yuan, X.-P.; Zhang, Q.-Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Zhou, G.-Z. <i>et al.</i>","Englishabstract":"A marine fish cell line from the snout of red spotted grouper <i>Epinephelus akaara</i>, a protogynous hermaphrodite, was established, characterized, and subcultured with more than 60 passages. The grouper snout cell line (GSC) cells multiplied well in Dulbecco’s modified Eagle’s medium (DMEM) medium supplemented with 10% fetal bovine serum. The optimal growth temperature was 25°C, and morphologically the cells were fibroblastic. Chromosome analysis revealed that the GSC cell line has a normal diploid karyotype with <a name=\"IEq1\"></a> <img src=\"/content/h8196k92319q6006/10126_2006_Article_7165_TeX2GIFIEq1.gif\" alt=\"$$ 2n = 8st + 40t $$\" align=\"middle\" border=\"0\">. A virus titration study indicated that the cells were susceptible to turbot <i>Scophthalmus Maximus</i> rhabdovirus (SMRV) (10<sup>8.5</sup> TCID<sub>50</sub> ml<sup>−1</sup>), while the viral titer of frog <i>Rana grylio</i> virus 9807 (RGV<sub>9807</sub>) reached 10<sup>3.5</sup> TCID<sub>50</sub> ml<sup>−1</sup>. The infection was confirmed by cytopathic effect (CPE), immunofluorescence, and electron microscopy experiments, which detected the viral particles in the cytoplasm of virus-infected cells, respectively. Further, significant fluorescent signals were observed when the GSC cells were transfected with pEGFP vector DNA, indicating their potential utility for transgenic and genetic manipulation studies.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (<i>Epinephelus akaara</i>)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-16 01:32:02.353374","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"fish cell line; iridovirus; red spotted grouper Epinephelus akaara;rhabdovirus; transfection","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2020-10-23","DateCreate":"2007-08-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000247207600008","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10126-006-7165-3"},"refs":null,"anarec":{"AnaID":113519,"PubliDate":2007,"Pagination":"370-376","XtraPublOfAnaID":null,"ISBN":null,"Volume":"9","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45426,"SerRR":"Marine Biotechnology. 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