{"refrec":{"BRefID":113521,"RR":"<b>Sellars, M.J.; Lyons, R.E.; Grewe, P.M.; Vuocolo, T.; Leeton, L.; Coman, G.J.; Degnan, B.M.; Preston, N.P.</b> (2007). A PL10 vasa-like gene in the Kuruma shrimp, <i>Marsupenaeus japonicus</i>, expressed during development and in adult gonad. <i>Mar. Biotechnol. 9(3)</i>: 377-387. <a href=\"https://dx.doi.org/10.1007/s10126-007-6118-9\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-007-6118-9</a>","BEntID":107977,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Biotechnol. 9(3)</i>: 377-387. <a href=\"https://dx.doi.org/10.1007/s10126-007-6118-9\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-007-6118-9</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Sellars, M.J.; Lyons, R.E.; Grewe, P.M.; Vuocolo, T.; Leeton, L.; Coman, G.J.; Degnan, B.M.; Preston, N.P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Sellars, M.J. <i>et al.</i>","Englishabstract":"A PL10 vasa-like gene was isolated from the Kuruma shrimp <i>Marsupenaeus japonicus</i> and therefore called <i>Mjpl10</i>. It is differentially expressed during embryonic, larval, and postlarval development, and in female and male gonads. Using absolute real-time reverse transcriptase-polymerase chain reaction (RT-PCR), we demonstrate that <i>Mjpl10</i> transcripts are present in the two-cell embryo, suggesting it is maternally expressed, and continually at low levels throughout embryogenesis. <i>Mjpl10</i> expression increases significantly in the first 25 h after hatching (nauplii IV) and then decreases in a linear fashion by 316-fold over the next 52-day period. Its continued expression throughout embryonic and larval development is compatible with a conserved role in early germ cell specification. Transcript levels of Mjpl10 are also detected in the ovary and testes of mature adults. 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