{"refrec":{"BRefID":115677,"RR":"<b>Geist, J.; Werner, I.; Eder, K.J.; Leutenegger, C.M.</b> (2007). Comparisons of tissue-specific transcription of stress response genes with whole animal endpoints of adverse effect in striped bass (<i>Morone saxatilis</i>) following treatment with copper and esfenvalerate. <i>Aquat. Toxicol. 85(1)</i>: 28-39. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2007.07.011\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2007.07.011</a>","BEntID":110052,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Toxicol. 85(1)</i>: 28-39. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2007.07.011\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2007.07.011</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Geist, J.; Werner, I.; Eder, K.J.; Leutenegger, C.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Geist, J. <i>et al.</i>","Englishabstract":"Changes in the gene transcription of stress response genes in resident fish can be powerful biomarkers for the identification of sublethal impacts of environmental stressors on aquatic ecosystems. In this study, we tested the effects of two reference toxicants, copper (Cu) and the pyrethroid insecticide esfenvalerate [(<i>S</i>)-α-cyano-3-phenoxybenzyl-(<i>S</i>)-2-(4-chlorophenyl)-3-methylbutyrate], on lethal (mortality) and sublethal endpoints (growth, swimming behavior, transcription levels of stress response genes) in juvenile (81–90-day-old) striped bass (<i>Morone saxatilis</i>). We established cellular stress response markers for proteotoxicity (HSP70, HSP90), phase I detoxification mechanism (CYP1A1), metal-binding (metallothionein), as well as immune-function and pathogen-defense (TGF-β, Mx-protein, nRAMP). Quantitative real-time TaqMan<sup>®</sup> PCR was used to examine tissue-specific changes in the transcriptome of liver, spleen, white muscle, anterior kidney and gills after 7-day Cu exposures and 24-h esfenvalerate exposures. On the transcriptome level, exposure to Cu showed strongest effects on the transcription of metallothionein in spleen tissue, causing a 4-fold increase of mRNA at 42 ppb total Cu and a 10-fold increase at 160 ppb Cu. Exposure to Cu also caused significant tissue-specific changes in gene transcription for immune-system related genes. Esfenvalerate exposure had tissue-specific effects on the transcription of HSP70, HSP90 and CYP1A1. The most significant effects were detected in liver tissue after exposure to 0.64 μg/L esfenvalerate.<p>Our results show that the stress response at the transcriptome level is a more sensitive indicator for Cu and esfenvalerate exposures at low concentrations than swimming behavior, growth or mortality. The accuracy of studies on quantitative changes in the transcriptome can benefit from an initial evaluation or the inclusion of several different tissues and the use of multiple housekeeping genes. ","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Comparisons of tissue-specific transcription of stress response genes with whole animal endpoints of adverse effect in striped bass (<i>Morone saxatilis</i>) following treatment with copper and esfenvalerate","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:31:34.114109","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"contaminants; cellular stress response; sublethal toxicity; biomarker;ecotoxicogenomics; transcriptome; behavior","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2020-10-23","DateCreate":"2007-10-19","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000250181300004","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2007.07.011"},"refs":null,"anarec":{"AnaID":115677,"PubliDate":2007,"Pagination":"28-39","XtraPublOfAnaID":null,"ISBN":null,"Volume":"85","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":28,"Pages":12,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Geist","Firstname":"Juergen","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":110052,"AutID":76416,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Werner","Firstname":"Inge","Initials":"I.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":110052,"AutID":54831,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Eder","Firstname":"Kai","Initials":"K.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":110052,"AutID":54830,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Leutenegger","Firstname":"Christian","Initials":"C.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":110052,"AutID":76417,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":353378,"LibID":36,"BRefID":115677,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"353378","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier Science","City":"Tokyo; New York; London; Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Behavior","ThestID":856,"Acronym":"ASFA","ThesTermPath":"Behavior"},{"ThesaurusTerm":"Biomarkers","ThestID":9475,"Acronym":"ASFA","ThesTermPath":"Biomarkers"},{"ThesaurusTerm":"Contaminants","ThestID":55147,"Acronym":"CSA","ThesTermPath":"Contaminants"},{"ThesaurusTerm":"Contaminants","ThestID":96109,"Acronym":"TRT","ThesTermPath":"Contaminants"},{"ThesaurusTerm":"Contaminants","ThestID":106723,"Acronym":"CAB","ThesTermPath":"Products > Contaminants"},{"ThesaurusTerm":"Sublethal effects","ThestID":8114,"Acronym":"ASFA","ThesTermPath":"Sublethal effects"},{"ThesaurusTerm":"Transcriptome","ThestID":166758,"Acronym":"CAB","ThesTermPath":"Transcriptome"}],"thestermsASFA":[{"ThesaurusTerm":"Biomarkers"},{"ThesaurusTerm":"Contaminants"},{"ThesaurusTerm":"Contaminants"},{"ThesaurusTerm":"Contaminants"},{"ThesaurusTerm":"Sublethal effects"},{"ThesaurusTerm":"Transcriptome"}],"taxtermsASFA":[{"TaxTerm":"Morone saxatilis (Walbaum, 1792)"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":110052,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":110052,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.aquatox.2007.07.011","externalID":"10.1016/j.aquatox.2007.07.011","URLTypeCode":"DOI","URLID":8742,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Behavior","ThestID":856,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Biomarkers","ThestID":9475,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Contaminants","ThestID":55147,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Contaminants","ThestID":96109,"Acronym":"TRT","ThesTypID":3,"ThesType":"Transportation Research Thesaurus"},{"ThesaurusTerm":"Contaminants","ThestID":106723,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Sublethal effects","ThestID":8114,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Transcriptome","ThestID":166758,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxterms":[{"TaxTerm":"Morone saxatilis (Walbaum, 1792) [Striped bass]","AphiaID":151179,"TaxtID":21898}],"geoterms":null,"othterms":[{"OtherTerm":"Cellular stress response","OthtID":17204},{"OtherTerm":"Ecotoxicogenomics","OthtID":17205},{"OtherTerm":"Sublethal toxicity","OthtID":10411}],"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Behavior","DutchTerm":"Gedrag","ThestID":856,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Biomarkers","DutchTerm":"Biomarkers","ThestID":9475,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Contaminants","DutchTerm":"Contaminanten","ThestID":55147,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Contaminants","DutchTerm":null,"ThestID":96109,"Acronym":"TRT","ThesTypID":3,"ThesType":"Transportation Research Thesaurus"},{"ThesaurusTerm":"Contaminants","DutchTerm":null,"ThestID":106723,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Sublethal effects","DutchTerm":null,"ThestID":8114,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Transcriptome","DutchTerm":null,"ThestID":166758,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxtermsFRIS":[{"TaxTerm":"Morone saxatilis (Walbaum, 1792)","DutchTerm":"gestreepte baars","AphiaID":151179,"TaxtID":21898}],"geotermsFRIS":null,"othtermsFRIS":[{"OtherTerm":"Cellular stress response","DutchTerm":null,"OthtID":17204},{"OtherTerm":"Ecotoxicogenomics","DutchTerm":null,"OthtID":17205},{"OtherTerm":"Sublethal toxicity","DutchTerm":null,"OthtID":10411}],"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2007-10-19 10:19:21.733000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2020-10-23 07:01:24.213000","timezone_type":3,"timezone":"Europe/Brussels"}}}