{"refrec":{"BRefID":116593,"RR":"<b>Shirai, Y.; Takao, Y.; Mizumoto, H.; Tomaru, Y.; Honda, D.; Nagasaki, K.</b> (2006). Genomic and phylogenetic analysis of a single-stranded RNA virus infecting <i>Rhizosolenia setigera</i> (Stramenopiles: Bacillariophyceae). <i>J. Mar. Biol. Ass. U.K. 86(3)</i>: 475-483. <a href=\"https://dx.doi.org/10.1017/S0025315406013373\" target=\"_blank\">https://dx.doi.org/10.1017/S0025315406013373</a>","BEntID":110911,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Mar. Biol. Ass. U.K. 86(3)</i>: 475-483. <a href=\"https://dx.doi.org/10.1017/S0025315406013373\" target=\"_blank\">https://dx.doi.org/10.1017/S0025315406013373</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Shirai, Y.; Takao, Y.; Mizumoto, H.; Tomaru, Y.; Honda, D.; Nagasaki, K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Shirai, Y. <i>et al.</i>","Englishabstract":"We report the first complete genome sequence of the marine diatom-infecting, positive-sense single-stranded RNA (ssRNA) virus, <i>Rhizosolenia setigera</i> RNA virus (RsRNAV). The genome is 8877 nucleotides (nt), polyadenylated, lacking a cap structure, and has two large open reading frames (ORFs): ORF-1 (4818 nt), a polyprotein gene coding for replicases, e.g. RNA helicase, RNA-dependent RNA polymerase (RdRp); and ORF-2 (2883 nt), a polyprotein gene coding for structural proteins. The ORFs are separated by a 323 nt intergenic region (IGR), flanked by a 624 nt 5'-untranslated region (UTR) and a 229 nt 3'-UTR. The deduced amino acid sequences for ORF-1 and ORF-2 respectively show considerable similarities to the non-structural and structural proteins of a marine raphidophyte-infecting virus HaRNAV (<i>Heterosigma akashiwo</i> RNA virus). Phylogenetic analyses of concatenated amino acid sequences of RNA helicase and RdRp domains supported the monophyly of RsRNAV, HaRNAV and a marine protist-infecting virus SssRNAV (<i>Schizochytrium</i> single-stranded RNA virus) with moderate bootstrap values of 79-83%, but not at the family level, whilst their monophyly was only weakly supported (50-55%) in the phylogenetic tree based on RdRp whole domain. As a result, comparison of the genome organization and sequence suggests RsRNAV is not a member of any currently defined virus family. In the RdRp tree, the positive-sense ssRNA viruses infecting Stramenopiles (RsRNAV, HaRNAV and SssRNAV) and Alveolata (HcRNAV (<i>Heterocapsa circularisquama</i> RNA virus)) were categorized into phylogenetically distant clades, which suggests a host/virus coevolution. Our study supports the hypothesis that a diverse array of ssRNA viruses exists in marine environments.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Genomic and phylogenetic analysis of a single-stranded RNA virus infecting <i>Rhizosolenia setigera</i> (Stramenopiles: Bacillariophyceae)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-27 01:31:01.157608","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2006,"MonPub":null,"DateUpdate":"2020-10-23","DateCreate":"2007-11-08","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000237149800004","VABBcode":null,"OpenAcc":0,"DOI":"10.1017/S0025315406013373"},"refs":null,"anarec":{"AnaID":116593,"PubliDate":2006,"Pagination":"475-483","XtraPublOfAnaID":null,"ISBN":null,"Volume":"86","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43277,"SerRR":"Journal of the Marine Biological Association of the United Kingdom. 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