{"refrec":{"BRefID":117341,"RR":"<b>Ip, D.T.-M.; Wong, K.-B.; Wan, D.C.-C.</b> (2007). Characterization of novel orange fluorescent protein cloned from cnidarian tube anemone <i>Cerianthus</i> sp. <i>Mar. Biotechnol. 9(4)</i>: 469-478. <a href=\"https://dx.doi.org/10.1007/s10126-007-9005-5\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-007-9005-5</a>","BEntID":111653,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Biotechnol. 9(4)</i>: 469-478. <a href=\"https://dx.doi.org/10.1007/s10126-007-9005-5\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-007-9005-5</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ip, D.T.-M.; Wong, K.-B.; Wan, D.C.-C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ip, D.T.-M.; Wong, K.-B.; Wan, D.C.-C.","Englishabstract":"A novel orange fluorescent protein (OFP) was cloned from the tentacles of Cnidarian tube anemone Cerianthus sp. It consists of 222 amino acid residues with a calculated molecular mass of 25.1 kDa. A BLAST protein sequence homology search revealed that native OFP has 81% sequence identity to <i>Cerianthus</i> membranaceus green fluorescent protein (cmFP512), 38% identity to <i>Entacmaea quadricolor</i> red fluorescent protein (eqFP611), 37% identity to <i>Discosoma</i> red fluorescent protein (DsRed), 36% identity to <i>Fungia concinna</i> Kusabira-orange fluorescent protein (KO), and a mere 21% identity to green fluorescent protein (GFP). It is most likely that OFP also adopts the 11-strand β-barrel structure of fluorescent proteins. Spectroscopic analysis indicated that it has a wide absorption spectrum peak at 548 nm with two shoulders at 487 and 513 nm. A bright orange fluorescence maximum at 573 nm was observed when OFP was excited at 515 nm or above. When OFP was excited well below 515 nm, a considerable amount of green emission maximum at 513 nm was also observed. It has a fluorescence quantum yield (Φ) of 0.64 at 25°C. The molar absorption coefficients (ε) of folded OFP at 278 and 548 nm are 47,000 and 60,000 M<sup>-1</sup> ·cm<sup>-1</sup>, respectively. Its fluorescent brightness (ε Φ) at 25°C is 38,400 M<sup>-1</sup> cm<sup>-1</sup>. Like other orange-red fluorescent proteins, OFP is also tetrameric. It was readily expressed as soluble protein in <i>Escherichia coli</i> at 37°C, and no aggregate was observed in transfected HeLa cells under our experimental conditions. Fluorescent intensity of OFP is detectable over a pH range of 3 to 12.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Characterization of novel orange fluorescent protein cloned from cnidarian tube anemone <i>Cerianthus</i> sp","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:31:34.457891","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Cerianthus; cnidarian; green fluorescent protein; orange fluorescentprotein","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2020-10-26","DateCreate":"2007-11-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000249731400008","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10126-007-9005-5"},"refs":null,"anarec":{"AnaID":117341,"PubliDate":2007,"Pagination":"469-478","XtraPublOfAnaID":null,"ISBN":null,"Volume":"9","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45426,"SerRR":"Marine Biotechnology. Springer-Verlag: New York.  ISSN 1436-2228; e-ISSN 1436-2236","StandardTitleSer":"Marine Biotechnology","ISSN":"1436-2228","AbbrevSer":"Mar. Biotechnol.","StandardTitleMon":null,"StartPage":469,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Ip","Firstname":"Denis Tsz-Ming","Initials":"D.T.-M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":111653,"AutID":77273,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Wong","Firstname":"Kam-Bo","Initials":"K.-B.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":111653,"AutID":77274,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Wan","Firstname":"David Chi-Cheong","Initials":"D.C.-C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":111653,"AutID":77275,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":353567,"LibID":36,"BRefID":117341,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"353567","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Springer-Verlag","City":"New York"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Green fluorescent protein","ThestID":152519,"Acronym":"CAB","ThesTermPath":"Peptides > Proteins > Marker proteins > Reporter proteins > Green fluorescent protein"}],"thestermsASFA":[{"ThesaurusTerm":"Green fluorescent protein"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":111653,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":111653,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/s10126-007-9005-5","externalID":"10.1007/s10126-007-9005-5","URLTypeCode":"DOI","URLID":9090,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Green fluorescent protein","ThestID":152519,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxterms":null,"geoterms":null,"othterms":[{"OtherTerm":"Cerianthus","OthtID":17504},{"OtherTerm":"Cnidarian","OthtID":16632},{"OtherTerm":"Orange fluorescent protein","OthtID":17505}],"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Green fluorescent protein","DutchTerm":null,"ThestID":152519,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":[{"OtherTerm":"Cerianthus","DutchTerm":null,"OthtID":17504},{"OtherTerm":"Cnidarian","DutchTerm":null,"OthtID":16632},{"OtherTerm":"Orange fluorescent protein","DutchTerm":null,"OthtID":17505}],"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2007-11-29 11:01:00.430000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2020-10-26 06:47:16.007000","timezone_type":3,"timezone":"Europe/Brussels"}}}