{"refrec":{"BRefID":127371,"RR":"<b>Fan, C.X.; Chen, X.K.; Zhang, C.; Wang, L.X.; Duan, K.L.; He, L.L.; Cao, Y.; Liu, S.Y.; Zhong, M.N.; Ulens, C.; Tytgat, J.; Chen, J.S.; Chi, C.W.; Zhou, Z.</b> (2003). A novel conotoxin from <i>Conus betulinus</i>, kappa-BtX, unique in cysteine pattern and in function as a specific BK channel modulator. <i>J. Biol. Chem. 278(15)</i>: 12624-12633. <a href=\"https://dx.doi.org/10.1074/jbc.M210200200\" target=\"_blank\">https://dx.doi.org/10.1074/jbc.M210200200</a>","BEntID":121440,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Biol. Chem. 278(15)</i>: 12624-12633. <a href=\"https://dx.doi.org/10.1074/jbc.M210200200\" target=\"_blank\">https://dx.doi.org/10.1074/jbc.M210200200</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Fan, C.X.; Chen, X.K.; Zhang, C.; Wang, L.X.; Duan, K.L.; He, L.L.; Cao, Y.; Liu, S.Y.; Zhong, M.N.; Ulens, C.; Tytgat, J.; Chen, J.S.; Chi, C.W.; Zhou, Z.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Fan, C.X. <i>et al.</i>","Englishabstract":"A novel conotoxin, kappa-conotoxin (kappa-BtX), has been purified and characterized from the venom of a worm-hunting cone snail, <i>Conus betulinus</i>. The toxin, with four disulfide bonds, shares no sequence homology with any other conotoxins. Based on a partial amino acid sequence, its cDNA was cloned and sequenced. The deduced sequence consists of a 26-residue putative signal peptide, a 31-residue mature toxin, and a 13-residue extra peptide at the C terminus. The extra peptide is cleaved off by proteinase post-processing. All three Glu residues are gamma-carboxylated, one of the two Pro residues is hydroxylated at position 27, and its C-terminal residue is Pro-amidated. The monoisotopic mass of the toxin is 3569.0 Da. Electrophysiological experiments show that: 1) among voltage-gated channels;, K-BtX is a specific modulator of K+ channels; 2) among the K channels, kappa-BtX specifically up-modulates the Ca2+- and voltage-sensitive BK channels (252 47%); 3) its EC50 is 0.7 nm with a single binding site (Hill = 0.88); 4) the time constant of wash-out is 8.3 s; and 5) kappa-BtX has no effect on single channel conductance, but increases the open probability of BK channels. It is concluded that kappa-BtX is a novel specific biotoxin against BK channels.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A novel conotoxin from <i>Conus betulinus</i>, kappa-BtX, unique in cysteine pattern and in function as a specific BK channel modulator","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-17 01:31:43.067358","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2003,"MonPub":null,"DateUpdate":"2012-12-07","DateCreate":"2008-11-04","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000182189500007","VABBcode":null,"OpenAcc":0,"DOI":"10.1074/jbc.M210200200"},"refs":null,"anarec":{"AnaID":127371,"PubliDate":2003,"Pagination":"12624-12633","XtraPublOfAnaID":null,"ISBN":null,"Volume":"278","Issue":"15","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43206,"SerRR":"Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology: Baltimore, etc..  ISSN 0021-9258; e-ISSN 1083-351X","StandardTitleSer":"Journal of Biological Chemistry","ISSN":"0021-9258","AbbrevSer":"J. Biol. Chem.","StandardTitleMon":null,"StartPage":12624,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Fan","Firstname":null,"Initials":"C.X.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83622,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Chen","Firstname":null,"Initials":"X.K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83623,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Zhang","Firstname":null,"Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83624,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Wang","Firstname":null,"Initials":"L.X.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83625,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Duan","Firstname":null,"Initials":"K.L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83626,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"He","Firstname":null,"Initials":"L.L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83627,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Cao","Firstname":null,"Initials":"Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83628,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Liu","Firstname":null,"Initials":"S.Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83629,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Zhong","Firstname":null,"Initials":"M.N.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83630,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Ulens","Firstname":"Chris","Initials":"C.","Affiliation":"Laboratory of Toxicology, University of Leuven","Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83631,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"KU Leuven; Departement Farmaceutische en Farmacologische Wetenschappen; Laboratorium voor Toxicologie en Farmacologie","ORCID":null,"PersID":35778,"InsID":492},{"AutName":"Tytgat","Firstname":"Jan","Initials":"J.","Affiliation":"Laboratory of Toxicology, University of Leuven","Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":155270,"OrderNr":11,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"KU Leuven; Departement Farmaceutische en Farmacologische Wetenschappen; Laboratorium voor Toxicologie en Farmacologie","ORCID":null,"PersID":717,"InsID":492},{"AutName":"Chen","Firstname":null,"Initials":"J.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83632,"OrderNr":12,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Chi","Firstname":null,"Initials":"C.W.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83633,"OrderNr":13,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Zhou","Firstname":null,"Initials":"Z.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":121440,"AutID":83634,"OrderNr":14,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":140433,"LibID":36,"BRefID":127371,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"140433","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"American Society for Biochemistry and Molecular Biology","City":"Baltimore, etc."}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Biological poisons","ThestID":976,"Acronym":"ASFA","ThesTermPath":"Materials > Hazardous materials > Biological poisons"}],"thestermsASFA":[{"ThesaurusTerm":"Biological poisons"}],"taxtermsASFA":[{"TaxTerm":"Conidae"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":121440,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":121440,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1074/jbc.M210200200","externalID":"10.1074/jbc.M210200200","URLTypeCode":"DOI","URLID":111458,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Biological poisons","ThestID":976,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Conidae [turrids]","AphiaID":14107,"TaxtID":22534}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Biological poisons","DutchTerm":null,"ThestID":976,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Conidae","DutchTerm":null,"AphiaID":14107,"TaxtID":22534}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Haspeslagh, Jan, J.","newSesDate":{"date":"2008-11-04 09:34:51.020000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2012-12-07 13:34:29.183000","timezone_type":3,"timezone":"Europe/Brussels"}}}