{"refrec":{"BRefID":127765,"RR":"Sun, G.; Zhang, X.; Sui, Z.; Mao, Y. (2008). Inhibition of pds gene expression via the RNA interference approach in Dunaliella salina (Chlorophyta). Mar. Biotechnol. 10(3): 219-226. https://dx.doi.org/10.1007/s10126-007-9056-7","BEntID":121847,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". Mar. Biotechnol. 10(3): 219-226. https://dx.doi.org/10.1007/s10126-007-9056-7","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Sun, G.; Zhang, X.; Sui, Z.; Mao, Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Sun, G. et al.","Englishabstract":"To investigate the potential of double-stranded RNA interferencing with gene expression in Dunaliella salina, a plasmid pBIRNAI-Dsa was constructed to express hairpin RNA (hpRNA) containing sequences homologous to phytoene desaturase gene (pds), a key gene in carotenoid biosynthesis, and transformed into D. salinaby electroporation. The relative transcription level of pds in pBIRNAI-Dsa-treated cells to nontreated cells was quantitated and the gene silencing efficiency by RNAi was evaluated via real-time polymerase chain reaction (PCR). The transcriptions of pds of the pBIRNAI-Dsa-treated group changed compared to those of the control group, and the was lowest on the 7th day, corresponding to 0.281265-fold of the relative pds control transcript; a relatively strong gene inhibition effect was therefore deduced. The transcript of pds may be modulated in a wide range, and a reduced transcription even to 28% of the normal level may be tolerated for its survival. This study shows that dsRNA-mediated genetic interference can induce sequence-specific inhibition of gene expression and pBIRNAI-Dsa can be used for transient suppression of gene expression inD. salina. The aim of this study was to exploit dsRNA-mediated gene silencing and to provide a foundation for gene function research in D. salina.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Inhibition of pds gene expression via the RNA interference approach in Dunaliella salina (Chlorophyta)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-15 01:32:08.129626","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2008,"MonPub":null,"DateUpdate":"2020-10-29","DateCreate":"2008-11-26","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000254905500001","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10126-007-9056-7"},"refs":null,"anarec":{"AnaID":127765,"PubliDate":2008,"Pagination":"219-226","XtraPublOfAnaID":null,"ISBN":null,"Volume":"10","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45426,"SerRR":"Marine Biotechnology. 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