{"refrec":{"BRefID":128658,"RR":"<b>Bossuyt, B.T.A.; Escobar, YR.; Janssen, C.R.<\/b> (2005). Multigeneration acclimation of <i>Daphnia magna Straus<\/i> to different bioavailable copper concentrations. <i>Ecotoxicol. Environ. Saf. 61(3)<\/i>: 327-336. <a href=\"https:\/\/dx.doi.org\/10.1016\/j.ecoenv.2005.03.004\" target=\"_blank\">https:\/\/dx.doi.org\/10.1016\/j.ecoenv.2005.03.004<\/a>","BEntID":122747,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Ecotoxicol. Environ. Saf. 61(3)<\/i>: 327-336. <a href=\"https:\/\/dx.doi.org\/10.1016\/j.ecoenv.2005.03.004\" target=\"_blank\">https:\/\/dx.doi.org\/10.1016\/j.ecoenv.2005.03.004<\/a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":1,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bossuyt, B.T.A.; Escobar, YR.; Janssen, C.R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bossuyt, B.T.A.; Escobar, YR.; Janssen, C.R.","Englishabstract":"A large acclimation experiment was performed with <i>Daphnia magna<\/i> in which two different copper bioavailability (as Cu<sup>2+<\/sup>) groups (N and M) were used. In the N group the cupric ion activity increased with increasing dissolved copper-acclimation concentration, while in the M group the ion activity decreased with increasing dissolved copper concentration. The activity of copper carbonates and hydroxides was up to an order of magnitude lower than the cupric ion activity. After five generations of acclimation, the acute copper sensitivity (mean\u00b1SD) of the N group ranged from 193\u00b124 to 296\u00b150\u03bcg CuL<sup>-1<\/sup> and for the M group from 198\u00b127 to 315\u00b138 \u03bcg CuL<sup>-1<\/sup> for daphnids acclimated to 1 and 100\u03bcg CuL<sup>-1<\/sup>, respectively. The internal copper concentration of the acclimated daphnids also resulted in similar results between the two groups. Acclimation of the two daphnid groups for five consecutive generations to the three dissolved copper concentrations resulted in a shift in the optimal concentration range toward 1 \u03bcg CuL<sup>-1<\/sup>, using energy reserves as an endpoint. 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