Document of bibliographic reference 128708

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Long-term acclimation of Pseudokirchneriella subcapitata (Korshikov) Hindak to different copper concentrations: changes in tolerance and physiology

Abstract

The effect of long-term copper acclimation of the freshwater green algae Pseudokirchneriella subcapitata to copper was investigated using different physiological and toxicological endpoints. The algae were exposed to seven-five of which are ecologically relevant for European surface waters-copper concentration ranging from 0.5 to 100 µg Cu l^-1 during a 3-month period. A standard medium was used as culture and test medium with an addition of 2 mg DOC l^-1(replacing EDTA). At certain intervals, experiments were performed to assess algal biomass, growth rate, chlorophyll and carotenoid content, pigment diversity, autotrophic index, intracellular and adsorbed copper, and the sensitivity of the algae to copper. Chronic copper tolerance (mean ± standard deviation) increased significantly from 88 ± 15 to 124 ± 25 µg Cu l^-1 for P. subcapitata acclimated to 0.5 and 100 µg Cu l^-1, respectively. Based on the algal biomass, the growth rate, the pigment diversity and the autotrophic index, an optimal concentration range was observed between 1 and 35 µg Cu l^-1. Significant decreases in algal biomass, pigment diversity and autotrophic index were observed in algal cultures acclimated to 0.5 tag Cu l^-1 and 100 tag Cu l^-1. Chlorophyll a content (mean ± standard deviation) increased from 8.4 ± 3.1 to 28.6 ± 7.5 x 10^-14 g per cell and carotenoid content (mean ± standard deviation) increased from 3.7 ± 0.8 to 7.1 ± 1.2 x 10^-14 g per cell for algae exposed to 1 and 100 µg Cu l^-1, respectively. Intracellular copper increased from 0.099 to 20.6 x 10^-15 g Cu per cell and adsorbed copper increased from 0.026 to 1.8 x 10^-1 g Cu per cell for algae acclimated for 12 weeks to 0.5 and 100 tag Cu l^-1, respectively. This research demonstrates that the use of standard culture media, some of which may be deficient in copper, can result in sub-optimal performance of the organisms, which in turn may affect toxicity test results. Additionally, this work also established an optimal concentration range for copper for this algal species. This phenomenon should be taken in consideration when performing environmental risk assessments of essential elements.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000221549500006

Bibliographic citation

Bossuyt, B.T.A.; Janssen, C.R. (2004). Long-term acclimation of Pseudokirchneriella subcapitata (Korshikov) Hindak to different copper concentrations: changes in tolerance and physiology. Aquat. Toxicol. 68(1): 61-74. dx.doi.org/10.1016/j.aquatox.2004.02.005

Topic

Fresh water

Is peer reviewed

true